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@article{1410440,
author = {Večeřa, Josef and Kudová, Jana and Kučera, Jan and Kubala, Lukáš and Pacherník, Jiří},
article_location = {London},
article_number = {prosinec},
doi = {http://dx.doi.org/10.1155/2017/8715798},
keywords = {STEM-CELL FATE; HYPOXIA; OXYGEN; HIF; CATENIN},
language = {eng},
issn = {1687-966X},
journal = {Stem Cells International},
title = {Neural Differentiation Is Inhibited through HIF1 alpha/ beta-Catenin Signaling in Embryoid Bodies},
url = {http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=E5gWduQPo6sNAt8bJHA&page=1&doc=4},
volume = {Neuveden},
year = {2017}
}
TY - JOUR
ID - 1410440
AU - Večeřa, Josef - Kudová, Jana - Kučera, Jan - Kubala, Lukáš - Pacherník, Jiří
PY - 2017
TI - Neural Differentiation Is Inhibited through HIF1 alpha/ beta-Catenin Signaling in Embryoid Bodies
JF - Stem Cells International
VL - Neuveden
IS - prosinec
SP - nestránkováno
EP - nestránkováno
PB - HINDAWI LTD
SN - 1687966X
KW - STEM-CELL FATE
KW - HYPOXIA
KW - OXYGEN
KW - HIF
KW - CATENIN
UR - http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=E5gWduQPo6sNAt8bJHA&page=1&doc=4
N2 - Extensive research in the field of stem cells and developmental biology has revealed evidence of the role of hypoxia as an important factor regulating self-renewal and differentiation. However, comprehensive information about the exact hypoxia-mediated regulatory mechanism of stem cell fate during early embryonic development is still missing. Using a model of embryoid bodies (EBs) derived from murine embryonic stem cells (ESC), we here tried to encrypt the role of hypoxia-inducible factor 1 alpha (HIF1 alpha) in neural fate during spontaneous differentiation. EBs derived from ESC with the ablated gene for HIF1a had abnormally increased neuronal characteristics during differentiation. An increased neural phenotype in Hif1 alpha(-/-) EBs was accompanied by the disruption of beta-catenin signaling together with the increased cytoplasmic degradation of beta-catenin. The knock-in of Hif1 alpha, as well as beta-catenin ectopic overexpression in Hif1 alpha(-/-) EBs, induced a reduction in neural markers to the levels observed in wild-type EBs. Interestingly, direct interaction between HIF1 alpha and beta-catenin was demonstrated by immunoprecipitation analysis of the nuclear fraction of wild-type EBs. Together, these results emphasize the regulatory role of HIF1 alpha in beta-catenin stabilization during spontaneous differentiation, which seems to be a crucial mechanism for the natural inhibition of premature neural differentiation.
ER -
VEČEŘA, Josef, Jana KUDOVÁ, Jan KUČERA, Lukáš KUBALA a Jiří PACHERNÍK. Neural Differentiation Is Inhibited through HIF1 alpha/ beta-Catenin Signaling in Embryoid Bodies. \textit{Stem Cells International}. London: HINDAWI LTD, Neuveden, prosinec, s.~nestránkováno, 12 s. ISSN~1687-966X. doi:10.1155/2017/8715798. 2017.
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