Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by
a Physical Association with the Ku Heterodimer

J 2017

Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer

VALUCHOVÁ, Soňa, Jaroslav FULNEČEK, Zbyněk PROKOP, Peggy STOLT-BERGNER, Eliška JANOUŠKOVÁ et. al.

Basic information

Original name

Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer

Authors

VALUCHOVÁ, Soňa (703 Slovakia, belonging to the institution), Jaroslav FULNEČEK (203 Czech Republic, belonging to the institution), Zbyněk PROKOP (203 Czech Republic, belonging to the institution), Peggy STOLT-BERGNER (40 Austria), Eliška JANOUŠKOVÁ (203 Czech Republic, belonging to the institution), Ctirad HOFR (203 Czech Republic, belonging to the institution) and Karel ŘÍHA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plant Cell, ROCKVILLE, AMER SOC PLANT BIOLOGISTS, 2017, 1040-4651

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 8.228

RIV identification code

RIV/00216224:14740/17:00095562

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1105/tpc.17.00064

UT WoS

000404989600026

Keywords in English

STRAND BREAK REPAIR; INDUCED FLUORESCENCE ENHANCEMENT; SINGLE DNA END; MAMMALIAN TELOMERES; PROTEIN; LENGTH; COMPLEX; DAMAGE; FUSIONS; YEAST

Abstract

V originále

Telomeres form specialized chromatin that protects natural chromosome termini from being recognized as DNA double-strand breaks. Plants possess unusual blunt-ended telomeres that are unable to form t-loops or complex with single-strand DNA binding proteins, raising the question of the mechanism behind their protection. We have previously suggested that blunt-ended telomeres in Arabidopsis thaliana are protected by Ku, a DNA repair factor with a high affinity for DNA ends. In nonhomologous end joining, Ku loads onto broken DNA via a channel consisting of positively charged amino acids. Here, we demonstrate that while association of Ku with plant telomeres also depends on this channel, Ku's requirements for DNA binding differ between DNA repair and telomere protection. We show that a Ku complex proficient in DNA loading but impaired in translocation along DNA is able to protect blunt-ended telomeres but is deficient in DNA repair. This suggests that Ku physically sequesters blunt-ended telomeres within its DNA binding channel, shielding them from other DNA repair machineries.

Links

GAP205/12/0550, research and development project

Name: Dynamika vzniku shelterinového komplexu

Investor: Czech Science Foundation

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

1304130933, interní kód MU

Name: Blunt-ended telomeres: dissecting the novel mechanism of chromosome end protection

Investor: EMBO (European Molecular Biology Organization)

Citovat

VALUCHOVÁ, Soňa, Jaroslav FULNEČEK, Zbyněk PROKOP, Peggy STOLT-BERGNER, Eliška JANOUŠKOVÁ, Ctirad HOFR and Karel ŘÍHA. Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer. Plant Cell. ROCKVILLE: AMER SOC PLANT BIOLOGISTS, 2017, vol. 29, No 6, p. 1533-1545. ISSN 1040-4651. Available from: https://dx.doi.org/10.1105/tpc.17.00064.

@article{1410576,
   author = {Valuchová, Soňa and Fulneček, Jaroslav and Prokop, Zbyněk and StoltandBergner, Peggy and Janoušková, Eliška and Hofr, Ctirad and Říha, Karel},
   article_location = {ROCKVILLE},
   article_number = {6},
   doi = {http://dx.doi.org/10.1105/tpc.17.00064},
   keywords = {STRAND BREAK REPAIR; INDUCED FLUORESCENCE ENHANCEMENT; SINGLE DNA END; MAMMALIAN TELOMERES; PROTEIN; LENGTH; COMPLEX; DAMAGE; FUSIONS; YEAST},
   language = {eng},
   issn = {1040-4651},
   journal = {Plant Cell},
   title = {Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer},
   url = {http://www.plantcell.org/content/29/6/1533},
   volume = {29},
   year = {2017}
}

TY  - JOUR
ID  - 1410576
AU  - Valuchová, Soňa - Fulneček, Jaroslav - Prokop, Zbyněk - Stolt-Bergner, Peggy - Janoušková, Eliška - Hofr, Ctirad - Říha, Karel
PY  - 2017
TI  - Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer
JF  - Plant Cell
VL  - 29
IS  - 6
SP  - 1533-1545
EP  - 1533-1545
PB  - AMER SOC PLANT BIOLOGISTS
SN  - 10404651
KW  - STRAND BREAK REPAIR
KW  - INDUCED FLUORESCENCE ENHANCEMENT
KW  - SINGLE DNA END
KW  - MAMMALIAN TELOMERES
KW  - PROTEIN
KW  - LENGTH
KW  - COMPLEX
KW  - DAMAGE
KW  - FUSIONS
KW  - YEAST
UR  - http://www.plantcell.org/content/29/6/1533
L2  - http://www.plantcell.org/content/29/6/1533
N2  - Telomeres form specialized chromatin that protects natural chromosome termini from being recognized as DNA double-strand breaks. Plants possess unusual blunt-ended telomeres that are unable to form t-loops or complex with single-strand DNA binding proteins, raising the question of the mechanism behind their protection. We have previously suggested that blunt-ended telomeres in Arabidopsis thaliana are protected by Ku, a DNA repair factor with a high affinity for DNA ends. In nonhomologous end joining, Ku loads onto broken DNA via a channel consisting of positively charged amino acids. Here, we demonstrate that while association of Ku with plant telomeres also depends on this channel, Ku's requirements for DNA binding differ between DNA repair and telomere protection. We show that a Ku complex proficient in DNA loading but impaired in translocation along DNA is able to protect blunt-ended telomeres but is deficient in DNA repair. This suggests that Ku physically sequesters blunt-ended telomeres within its DNA binding channel, shielding them from other DNA repair machineries.
ER  -

VALUCHOVÁ, Soňa, Jaroslav FULNEČEK, Zbyněk PROKOP, Peggy STOLT-BERGNER, Eliška JANOUŠKOVÁ, Ctirad HOFR and Karel ŘÍHA. Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer. \textit{Plant Cell}. ROCKVILLE: AMER SOC PLANT BIOLOGISTS, 2017, vol.~29, No~6, p.~1533-1545. ISSN~1040-4651. Available from: https://dx.doi.org/10.1105/tpc.17.00064.

Detailed Information on Publication Record