Establishment of Myotis myotis Cell Lines - Model for
Investigation of Host-Pathogen Interaction in a Natural Host
for Emerging Viruses

J 2014

Establishment of Myotis myotis Cell Lines - Model for Investigation of Host-Pathogen Interaction in a Natural Host for Emerging Viruses

HE, Xiaocui; Tomas KORYTAR; Yaqing ZHU; Jiri PIKULA; Hana BANDOUCHOVA et al.

Základní údaje

Originální název

Establishment of Myotis myotis Cell Lines - Model for Investigation of Host-Pathogen Interaction in a Natural Host for Emerging Viruses

Autoři

HE, Xiaocui; Tomas KORYTAR; Yaqing ZHU; Jiri PIKULA; Hana BANDOUCHOVA; Jan ZUKAL a Bernd KOELLNER

Vydání

PLOS ONE, SAN FRANCISCO, Public Library of Science, 2014, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.234

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/14:00100366

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

BATS PTEROPUS-POLIOCEPHALUS; HUMAN RABIES; FRUIT BATS; INFECTION; LYSSAVIRUS; CORONAVIRUS; INTERFERON; EXPRESSION; RESERVOIRS; MICROGLIA

Štítky

NZ, rivok

Změněno: 13. 4. 2018 16:26, Ing. Nicole Zrilić

Anotace

V originále

Bats are found to be the natural reservoirs for many emerging viruses. In most cases, severe clinical signs caused by such virus infections are normally not seen in bats. This indicates differences in the virus-host interactions and underlines the necessity to develop natural host related models to study these phenomena. Due to the strict protection of European bat species, immortalized cell lines are the only alternative to investigate the innate anti-virus immune mechanisms. Here, we report about the establishment and functional characterization of Myotis myotis derived cell lines from different tissues: brain (MmBr), tonsil (MmTo), peritoneal cavity (MmPca), nasal epithelium (MmNep) and nervus olfactorius (MmNol) after immortalization by SV 40 large T antigen. The usefulness of these cell lines to study antiviral responses has been confirmed by analysis of their susceptibility to lyssavirus infection and the mRNA patterns of immune-relevant genes after poly I: C stimulation. Performed experiments indicated varying susceptibility to lyssavirus infection with MmBr being considerably less susceptible than the other cell lines. Further investigation demonstrated a strong activation of interferon mediated antiviral response in MmBr contributing to its resistance. The pattern recognition receptors: RIG-I and MDA5 were highly upregulated during rabies virus infection in MmBr, suggesting their involvement in promotion of antiviral responses. The presence of CD14 and CD68 in MmBr suggested MmBr cells are microglia-like cells which play a key role in host defense against infections in the central nervous system (CNS). Thus the expression pattern of MmBr combined with the observed limitation of lyssavirus replication underpin a protective mechanism of the CNS controlling the lyssavirus infection. Overall, the established cell lines are important tools to analyze antiviral innate immunity in M. myotis against neurotropic virus infections and present a valuable tool for a broad spectrum of future investigations in cellular biology of M. myotis.

Citovat

HE, Xiaocui; Tomas KORYTAR; Yaqing ZHU; Jiri PIKULA; Hana BANDOUCHOVA; Jan ZUKAL a Bernd KOELLNER. Establishment of Myotis myotis Cell Lines - Model for Investigation of Host-Pathogen Interaction in a Natural Host for Emerging Viruses. PLOS ONE. SAN FRANCISCO: Public Library of Science, 2014, roč. 9, č. 10, s. nestránkováno, 10 s. ISSN 1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0109795.

@article{1411149,
   author = {He, Xiaocui and Korytar, Tomas and Zhu, Yaqing and Pikula, Jiri and Bandouchova, Hana and Zukal, Jan and Koellner, Bernd},
   article_location = {SAN FRANCISCO},
   article_number = {10},
   doi = {https://doi.org/10.1371/journal.pone.0109795},
   keywords = {BATS PTEROPUS-POLIOCEPHALUS; HUMAN RABIES; FRUIT BATS; INFECTION; LYSSAVIRUS; CORONAVIRUS; INTERFERON; EXPRESSION; RESERVOIRS; MICROGLIA},
   language = {eng},
   issn = {1932-6203},
   journal = {PLOS ONE},
   title = {Establishment of Myotis myotis Cell Lines - Model for Investigation of Host-Pathogen Interaction in a Natural Host for Emerging Viruses},
   volume = {9},
   year = {2014}
}

TY  - JOUR
ID  - 1411149
AU  - He, Xiaocui - Korytar, Tomas - Zhu, Yaqing - Pikula, Jiri - Bandouchova, Hana - Zukal, Jan - Koellner, Bernd
PY  - 2014
TI  - Establishment of Myotis myotis Cell Lines - Model for Investigation of Host-Pathogen Interaction in a Natural Host for Emerging Viruses
JF  - PLOS ONE
VL  - 9
IS  - 10
SP  - nestránkováno
EP  - nestránkováno
PB  - Public Library of Science
SN  - 19326203
KW  - BATS PTEROPUS-POLIOCEPHALUS
KW  - HUMAN RABIES
KW  - FRUIT BATS
KW  - INFECTION
KW  - LYSSAVIRUS
KW  - CORONAVIRUS
KW  - INTERFERON
KW  - EXPRESSION
KW  - RESERVOIRS
KW  - MICROGLIA
N2  - Bats are found to be the natural reservoirs for many emerging viruses. In most cases, severe clinical signs caused by such virus infections are normally not seen in bats. This indicates differences in the virus-host interactions and underlines the necessity to develop natural host related models to study these phenomena. Due to the strict protection of European bat species, immortalized cell lines are the only alternative to investigate the innate anti-virus immune mechanisms. Here, we report about the establishment and functional characterization of Myotis myotis derived cell lines from different tissues: brain (MmBr), tonsil (MmTo), peritoneal cavity (MmPca), nasal epithelium (MmNep) and nervus olfactorius (MmNol) after immortalization by SV 40 large T antigen. The usefulness of these cell lines to study antiviral responses has been confirmed by analysis of their susceptibility to lyssavirus infection and the mRNA patterns of immune-relevant genes after poly I: C stimulation. Performed experiments indicated varying susceptibility to lyssavirus infection with MmBr being considerably less susceptible than the other cell lines. Further investigation demonstrated a strong activation of interferon mediated antiviral response in MmBr contributing to its resistance. The pattern recognition receptors: RIG-I and MDA5 were highly upregulated during rabies virus infection in MmBr, suggesting their involvement in promotion of antiviral responses. The presence of CD14 and CD68 in MmBr suggested MmBr cells are microglia-like cells which play a key role in host defense against infections in the central nervous system (CNS). Thus the expression pattern of MmBr combined with the observed limitation of lyssavirus replication underpin a protective mechanism of the CNS controlling the lyssavirus infection. Overall, the established cell lines are important tools to analyze antiviral innate immunity in M. myotis against neurotropic virus infections and present a valuable tool for a broad spectrum of future investigations in cellular biology of M. myotis.
ER  -

HE, Xiaocui; Tomas KORYTAR; Yaqing ZHU; Jiri PIKULA; Hana BANDOUCHOVA; Jan ZUKAL a Bernd KOELLNER. Establishment of Myotis myotis Cell Lines - Model for Investigation of Host-Pathogen Interaction in a Natural Host for Emerging Viruses. \textit{PLOS ONE}. SAN FRANCISCO: Public Library of Science, 2014, roč.~9, č.~10, s.~nestránkováno, 10 s. ISSN~1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0109795.

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