Absence of differences among low, middle, and high molecular
weight hyaluronan in activating murine immune cells in vitro

J 2018

Absence of differences among low, middle, and high molecular weight hyaluronan in activating murine immune cells in vitro

ŠAFRÁNKOVÁ, Barbora, M. HERMANNOVA, Kristina NEŠPOROVÁ, Vladimír VELEBNÝ, Lukáš KUBALA et. al.

Základní údaje

Originální název

Absence of differences among low, middle, and high molecular weight hyaluronan in activating murine immune cells in vitro

Autoři

ŠAFRÁNKOVÁ, Barbora (203 Česká republika), M. HERMANNOVA (203 Česká republika), Kristina NEŠPOROVÁ (203 Česká republika, domácí), Vladimír VELEBNÝ (203 Česká republika, domácí) a Lukáš KUBALA (203 Česká republika, garant, domácí)

Vydání

International journal of biological macromolecules, AMSTERDAM, Elsevier Science BV, Netherlands, 2018, 0141-8130

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.784

Kód RIV

RIV/00216224:14310/18:00102435

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.ijbiomac.2017.08.131

UT WoS

000423892200001

Klíčová slova anglicky

Inflammation; Phagocytes; Hyaluronan; Molecular weight

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 4. 2024 11:08, Mgr. Michal Petr

Anotace

V originále

Hyaluronan (HA) effects on immune response are suggested to be dependent on HA molecular weight (MW), as low MW HA should activate immune cells in contrast to high MW HA. However, some current studies do not support this conception and emphasize the importance of the form of preparation of HA, particularly with respect to its purity and origin. We compared the activation of mouse immune cells by HA samples (100 kDa, 500 kDa, and 997 kDa) prepared from HA originating from rooster comb, and HA samples (71 kDa, 500 kDa, and 1000 kDa) prepared from pharmacological grade HA originating from Streptococcus equi. Interestingly, in contrast to established theory, only middle and high MW HA originating from rooster comb induced the production of tumor necrosis factor-alpha by macrophages and in whole blood. Further, all tested preparations of HA failed to induce the expression of inducible nitric oxide synthase, the production of nitric oxide, or the expression of cyclooxygenase 2 in macrophages and splenocytes. Importantly, all HA samples originating from rooster comb were found to be contaminated by endotoxin (up to 1.23 EU/ml). Hence, low MW HA did not reveal itself to have significantly higher immunostimulatory activity compared to HA of higher MW. (C) 2017 Elsevier B.V. All rights reserved.

Citovat

ŠAFRÁNKOVÁ, Barbora, M. HERMANNOVA, Kristina NEŠPOROVÁ, Vladimír VELEBNÝ a Lukáš KUBALA. Absence of differences among low, middle, and high molecular weight hyaluronan in activating murine immune cells in vitro. International journal of biological macromolecules. AMSTERDAM: Elsevier Science BV, Netherlands, 2018, roč. 107, A, s. 1-8. ISSN 0141-8130. Dostupné z: https://dx.doi.org/10.1016/j.ijbiomac.2017.08.131.

@article{1411883,
   author = {Šafránková, Barbora and Hermannova, M. and Nešporová, Kristina and Velebný, Vladimír and Kubala, Lukáš},
   article_location = {AMSTERDAM},
   article_number = {A},
   doi = {http://dx.doi.org/10.1016/j.ijbiomac.2017.08.131},
   keywords = {Inflammation; Phagocytes; Hyaluronan; Molecular weight},
   language = {eng},
   issn = {0141-8130},
   journal = {International journal of biological macromolecules},
   title = {Absence of differences among low, middle, and high molecular weight hyaluronan in activating murine immune cells in vitro},
   url = {http://dx.doi.org/10.1016/j.ijbiomac.2017.08.131},
   volume = {107},
   year = {2018}
}

TY  - JOUR
ID  - 1411883
AU  - Šafránková, Barbora - Hermannova, M. - Nešporová, Kristina - Velebný, Vladimír - Kubala, Lukáš
PY  - 2018
TI  - Absence of differences among low, middle, and high molecular weight hyaluronan in activating murine immune cells in vitro
JF  - International journal of biological macromolecules
VL  - 107
IS  - A
SP  - 1-8
EP  - 1-8
PB  - Elsevier Science BV, Netherlands
SN  - 01418130
KW  - Inflammation
KW  - Phagocytes
KW  - Hyaluronan
KW  - Molecular weight
UR  - http://dx.doi.org/10.1016/j.ijbiomac.2017.08.131
N2  - Hyaluronan (HA) effects on immune response are suggested to be dependent on HA molecular weight (MW), as low MW HA should activate immune cells in contrast to high MW HA. However, some current studies do not support this conception and emphasize the importance of the form of preparation of HA, particularly with respect to its purity and origin. We compared the activation of mouse immune cells by HA samples (100 kDa, 500 kDa, and 997 kDa) prepared from HA originating from rooster comb, and HA samples (71 kDa, 500 kDa, and 1000 kDa) prepared from pharmacological grade HA originating from Streptococcus equi. Interestingly, in contrast to established theory, only middle and high MW HA originating from rooster comb induced the production of tumor necrosis factor-alpha by macrophages and in whole blood. Further, all tested preparations of HA failed to induce the expression of inducible nitric oxide synthase, the production of nitric oxide, or the expression of cyclooxygenase 2 in macrophages and splenocytes. Importantly, all HA samples originating from rooster comb were found to be contaminated by endotoxin (up to 1.23 EU/ml). Hence, low MW HA did not reveal itself to have significantly higher immunostimulatory activity compared to HA of higher MW. (C) 2017 Elsevier B.V. All rights reserved.
ER  -

ŠAFRÁNKOVÁ, Barbora, M. HERMANNOVA, Kristina NEŠPOROVÁ, Vladimír VELEBNÝ a Lukáš KUBALA. Absence of differences among low, middle, and high molecular weight hyaluronan in activating murine immune cells in vitro. \textit{International journal of biological macromolecules}. AMSTERDAM: Elsevier Science BV, Netherlands, 2018, roč.~107, A, s.~1-8. ISSN~0141-8130. Dostupné z: https://dx.doi.org/10.1016/j.ijbiomac.2017.08.131.

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