Genome-Wide miRNA Analysis Identifies miR-188-3p as a Novel
Prognostic Marker and Molecular Factor Involved in Colorectal
Carcinogenesis

J 2017

Genome-Wide miRNA Analysis Identifies miR-188-3p as a Novel Prognostic Marker and Molecular Factor Involved in Colorectal Carcinogenesis

PICHLER, M.; V. STIEGELBAUER; Petra VYCHYTILOVÁ; C. IVAN; H. LING et. al.

Základní údaje

Originální název

Genome-Wide miRNA Analysis Identifies miR-188-3p as a Novel Prognostic Marker and Molecular Factor Involved in Colorectal Carcinogenesis

Autoři

Vydání

Clinical cancer research, Philadelphia, AMER ASSOC CANCER RESEARCH, 2017, 1078-0432

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30204 Oncology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 10.199

Kód RIV

RIV/00216224:14740/17:00100443

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1158/1078-0432.CCR-16-0497

UT WoS

000396015600023

EID Scopus

2-s2.0-85014729382

Klíčová slova anglicky

ANTI-EGFR THERAPY; TUMOR-GROWTH; COLON-CANCER; MICRORNA SIGNATURES; PREDICTIVE-VALUE; STAGE-II; METASTASIS; EXPRESSION; PROGRESSION; BIOMARKER

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 4. 2021 13:41, Mgr. Tereza Miškechová

Anotace

V originále

Purpose: Characterization of colorectal cancer transcriptome by high-throughput techniques has enabled the discovery of several differentially expressed genes involving previously unreported miRNA abnormalities. Here, we followed a systematic approach on a global scale to identify miRNAs as clinical outcome predictors and further validated them in the clinical and experimental setting. Experimental Design: Genome-wide miRNA sequencing data of 228 colorectal cancer patients from The Cancer Genome Atlas dataset were analyzed as a screening cohort to identify miRNAs significantly associated with survival according to stringent pre-specified criteria. A panel of six miRNAs was further validated for their prognostic utility in a large independent validation cohort (n = 332). In situ hybridization and functional experiments in a panel of colorectal cancer cell lines and xenografts further clarified the role of clinical relevant miRNAs. Results: SixmiRNAs (miR-92b-3p, miR-188-3p, miR-221-5p, miR-331-3p, miR-425-3p, and miR-497-5p) were identified as strong predictors of survival in the screening cohort. High miR-188-3p expression proves to be an independent prognostic factor [screening cohort: HR = 4.137; 95% confidence interval (CI), 1.568-10.917; P = 0.004; validation cohort: HR = 1.538; 95% CI, 1.107-2.137; P = 0.010, respectively]. Forced miR-188-3p expression increased migratory behavior of colorectal cancer cells in vitro and metastases formation in vivo (P < 0.05). The promigratory role of miR-188-3p is mediated by direct interaction with MLLT4, a novel identified player involved in colorectal cancer cell migration. Conclusions: miR-188-3p is a novel independent prognostic factor in colorectal cancer patients, which can be partly explained by its effect on MLLT4 expression and migration of cancer cells. (C) 2016 AACR.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

90004, velká výzkumná infrastruktura

Název: BBMRI-CZ

Citovat

PICHLER, M.; V. STIEGELBAUER; Petra VYCHYTILOVÁ; C. IVAN; H. LING; E. WINTER; X.N. ZHANG; M. GOBLIRSCH; A. WULF-GOLDENBERG; M. OHTSUKA; J. HAYBAECK; M. SVOBODA; Y. OKUGAWA; A. GERGER; G. HOEFLER; A. GOEL; Ondřej SLABÝ a G.A. CALIN. Genome-Wide miRNA Analysis Identifies miR-188-3p as a Novel Prognostic Marker and Molecular Factor Involved in Colorectal Carcinogenesis. Clinical cancer research. Philadelphia: AMER ASSOC CANCER RESEARCH, 2017, roč. 23, č. 5, s. 1323-1333. ISSN 1078-0432. Dostupné z: https://doi.org/10.1158/1078-0432.CCR-16-0497.

@article{1411964,
   author = {Pichler, M. and Stiegelbauer, V. and Vychytilová, Petra and Ivan, C. and Ling, H. and Winter, E. and Zhang, X.N. and Goblirsch, M. and WulfandGoldenberg, A. and Ohtsuka, M. and Haybaeck, J. and Svoboda, M. and Okugawa, Y. and Gerger, A. and Hoefler, G. and Goel, A. and Slabý, Ondřej and Calin, G.A.},
   article_location = {Philadelphia},
   article_number = {5},
   doi = {https://doi.org/10.1158/1078-0432.CCR-16-0497},
   keywords = {ANTI-EGFR THERAPY; TUMOR-GROWTH; COLON-CANCER; MICRORNA SIGNATURES; PREDICTIVE-VALUE; STAGE-II; METASTASIS; EXPRESSION; PROGRESSION; BIOMARKER},
   language = {eng},
   issn = {1078-0432},
   journal = {Clinical cancer research},
   title = {Genome-Wide miRNA Analysis Identifies miR-188-3p as a Novel Prognostic Marker and Molecular Factor Involved in Colorectal Carcinogenesis},
   url = {http://clincancerres.aacrjournals.org/content/23/5/1323},
   volume = {23},
   year = {2017}
}

TY  - JOUR
ID  - 1411964
AU  - Pichler, M. - Stiegelbauer, V. - Vychytilová, Petra - Ivan, C. - Ling, H. - Winter, E. - Zhang, X.N. - Goblirsch, M. - Wulf-Goldenberg, A. - Ohtsuka, M. - Haybaeck, J. - Svoboda, M. - Okugawa, Y. - Gerger, A. - Hoefler, G. - Goel, A. - Slabý, Ondřej - Calin, G.A.
PY  - 2017
TI  - Genome-Wide miRNA Analysis Identifies miR-188-3p as a Novel Prognostic Marker and Molecular Factor Involved in Colorectal Carcinogenesis
JF  - Clinical cancer research
VL  - 23
IS  - 5
SP  - 1323-1333
EP  - 1323-1333
PB  - AMER ASSOC CANCER RESEARCH
SN  - 10780432
KW  - ANTI-EGFR THERAPY
KW  - TUMOR-GROWTH
KW  - COLON-CANCER
KW  - MICRORNA SIGNATURES
KW  - PREDICTIVE-VALUE
KW  - STAGE-II
KW  - METASTASIS
KW  - EXPRESSION
KW  - PROGRESSION
KW  - BIOMARKER
UR  - http://clincancerres.aacrjournals.org/content/23/5/1323
L2  - http://clincancerres.aacrjournals.org/content/23/5/1323
N2  - Purpose: Characterization of colorectal cancer transcriptome by high-throughput techniques has enabled the discovery of several differentially expressed genes involving previously unreported miRNA abnormalities. Here, we followed a systematic approach on a global scale to identify miRNAs as clinical outcome predictors and further validated them in the clinical and experimental setting. Experimental Design: Genome-wide miRNA sequencing data of 228 colorectal cancer patients from The Cancer Genome Atlas dataset were analyzed as a screening cohort to identify miRNAs significantly associated with survival according to stringent pre-specified criteria. A panel of six miRNAs was further validated for their prognostic utility in a large independent validation cohort (n = 332). In situ hybridization and functional experiments in a panel of colorectal cancer cell lines and xenografts further clarified the role of clinical relevant miRNAs. Results: SixmiRNAs (miR-92b-3p, miR-188-3p, miR-221-5p, miR-331-3p, miR-425-3p, and miR-497-5p) were identified as strong predictors of survival in the screening cohort. High miR-188-3p expression proves to be an independent prognostic factor [screening cohort: HR = 4.137; 95% confidence interval (CI), 1.568-10.917; P = 0.004; validation cohort: HR = 1.538; 95% CI, 1.107-2.137; P = 0.010, respectively]. Forced miR-188-3p expression increased migratory behavior of colorectal cancer cells in vitro and metastases formation in vivo (P < 0.05). The promigratory role of miR-188-3p is mediated by direct interaction with MLLT4, a novel identified player involved in colorectal cancer cell migration. Conclusions: miR-188-3p is a novel independent prognostic factor in colorectal cancer patients, which can be partly explained by its effect on MLLT4 expression and migration of cancer cells. (C) 2016 AACR.
ER  -

PICHLER, M.; V. STIEGELBAUER; Petra VYCHYTILOVÁ; C. IVAN; H. LING; E. WINTER; X.N. ZHANG; M. GOBLIRSCH; A. WULF-GOLDENBERG; M. OHTSUKA; J. HAYBAECK; M. SVOBODA; Y. OKUGAWA; A. GERGER; G. HOEFLER; A. GOEL; Ondřej SLABÝ a G.A. CALIN. Genome-Wide miRNA Analysis Identifies miR-188-3p as a Novel Prognostic Marker and Molecular Factor Involved in Colorectal Carcinogenesis. \textit{Clinical cancer research}. Philadelphia: AMER ASSOC CANCER RESEARCH, 2017, roč.~23, č.~5, s.~1323-1333. ISSN~1078-0432. Dostupné z: https://doi.org/10.1158/1078-0432.CCR-16-0497.

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