2018
Adipokinetic hormone and adenosine interfere with nematobacterial infection and locomotion in Drosophila melanogaster
IBRAHIM, Emad, Pavel DOBEŠ, Martin KUNC, Pavel HYRŠL, Dalibor KODRÍK et. al.Základní údaje
Originální název
Adipokinetic hormone and adenosine interfere with nematobacterial infection and locomotion in Drosophila melanogaster
Název česky
Adipokinetic hormone and adenosine interfere with nematobacterial infection and locomotion in Drosophila melanogaster
Autoři
IBRAHIM, Emad (818 Egypt), Pavel DOBEŠ (203 Česká republika, domácí), Martin KUNC (203 Česká republika, domácí), Pavel HYRŠL (203 Česká republika, garant, domácí) a Dalibor KODRÍK (203 Česká republika)
Vydání
Journal of Insect Physiology, Kidlington, England, Elsevier, 2018, 0022-1910
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30105 Physiology
Stát vydavatele
Velká Británie a Severní Irsko
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 2.862
Kód RIV
RIV/00216224:14310/18:00100913
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000434751100021
Klíčová slova česky
Adipokinetický hormon; adenosin; Drosophila; hlístice; oxidativní stres; lokomoce
Klíčová slova anglicky
Adipokinetic hormone; adenosine; Drosophila; nematode; oxidative stress; locomotion
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 23. 4. 2024 11:15, Mgr. Michal Petr
V originále
This study examined how adipokinetic hormone (AKH) and adenosine affect defense responses in Drosophila melanogaster larvae infected with entomopathogenic nematodes (EPN, Steinernema carpocapsae and Heterorhabditis bacteriophora). Three loss-of-function mutant larvae were tested: Akh1, AdoR1 (adenosine receptor), and Akh1 AdoR1. Mortality decreased in all mutants post-EPN infection compared with the control (w1118). Additionally, co-application of external AKH with EPN significantly increased mortality beyond rates observed in EPN-only treatment, while also elevating carbon dioxide production, a measure of metabolism. Furthermore trehalose levels increased in both w1118 and Akh1 larvae post-EPN infection, but the latter group exhibited a lower increase and total trehalose levels. Interestingly, baseline trehalose was relatively high in untreated AdoR1 and Akh1 AdoR1 mutants, with levels remaining unaffected by infection. Infection also elevated haemolymph lipid content overall, but the different mutations did not substantially influence this change. In contrast, haemolymph protein content dropped after EPN infection in all tested groups, but this decline was more intense among Akh1. In uninfected larvae mutations decreased antioxidative capacity in Akh1 and increased in AdoR1, however, its post-infection increases were similar in all mutants, suggesting that antioxidant response in Drosophila involves mechanisms also beyond AKH and adenosine. Furthermore, AKH application in w1118 larvae significantly increased movement distance and percentage of larval activity, but reduced velocity. Mutations of Akh and AdoR did not strongly affect locomotion.
Česky
This study examined how adipokinetic hormone (AKH) and adenosine affect defense responses in Drosophila melanogaster larvae infected with entomopathogenic nematodes (EPN, Steinernema carpocapsae and Heterorhabditis bacteriophora). Three loss-of-function mutant larvae were tested: Akh1, AdoR1 (adenosine receptor), and Akh1 AdoR1. Mortality decreased in all mutants post-EPN infection compared with the control (w1118). Additionally, co-application of external AKH with EPN significantly increased mortality beyond rates observed in EPN-only treatment, while also elevating carbon dioxide production, a measure of metabolism. Furthermore trehalose levels increased in both w1118 and Akh1 larvae post-EPN infection, but the latter group exhibited a lower increase and total trehalose levels. Interestingly, baseline trehalose was relatively high in untreated AdoR1 and Akh1 AdoR1 mutants, with levels remaining unaffected by infection. Infection also elevated haemolymph lipid content overall, but the different mutations did not substantially influence this change. In contrast, haemolymph protein content dropped after EPN infection in all tested groups, but this decline was more intense among Akh1. In uninfected larvae mutations decreased antioxidative capacity in Akh1 and increased in AdoR1, however, its post-infection increases were similar in all mutants, suggesting that antioxidant response in Drosophila involves mechanisms also beyond AKH and adenosine. Furthermore, AKH application in w1118 larvae significantly increased movement distance and percentage of larval activity, but reduced velocity. Mutations of Akh and AdoR did not strongly affect locomotion.
Návaznosti
| GA17-03253S, projekt VaV |
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