Entomopathogenic nematodes and insect immunity

HYRŠL, Pavel. Entomopathogenic nematodes and insect immunity. In 24th Helmintological days. 2018. ISBN 978-80-7444-057-1.

Základní údaje

• Originální název: Entomopathogenic nematodes and insect immunity
• Název česky: Entomopathogenic nematodes and insect immunity
• Autoři: HYRŠL, Pavel (203 Česká republika, garant, domácí).
• Vydání: 24th Helmintological days, 2018.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Konferenční abstrakt
• Obor: 10600 1.6 Biological sciences
• Stát vydavatele: Česká republika
• Utajení: není předmětem státního či obchodního tajemství
• Kód RIV: RIV/00216224:14310/18:00100939
• Organizační jednotka: Přírodovědecká fakulta
• ISBN: 978-80-7444-057-1
• Klíčová slova česky: entomopatogenní hlístice; imunita hmyzu; Heterorhabditis; Steinernema; Galleria; Drosophila
• Klíčová slova anglicky: entomopathogenic nematodes; insect immunity; Heterorhabditis; Steinernema; Galleria; Drosophila

Anotace

Entomopathogenic nematodes and their associated bacteria comprise together a highly pathogenic complex which is able to invade and kill insect host within two days. Both bacteria and nematodes produce a variety of factors interacting with the insect immune system and help to overcome host defences. The tripartite model (Drosophila, nematodes, bacteria) was established and used to show an immune function for candidate genes using different Drosophila mutants or RNAi lines with defects in clotting or other branches of the immune system. Microarray analysis was used to compare gene expression of Drosophila larvae infected by the entomopathogenic nematode Heterorhabditis bacteriophora and its symbiotic bacterium Photorhabdus luminescens with non-infected larvae. The role of candidate genes, selected based on genomic comparison, in response towards nematobacterial complex was further evaluated by in vivo infection assays. We demonstrated an immune function during nematode infection for known clotting enzymes and substrates, recognition molecules and eicosanoids. In conclusion, we show that the Heterorhabditis/Photorhabdus infection model is suitable to identify regulators of innate immunity in insects.

Anotace česky

Entomopathogenic nematodes and their associated bacteria comprise together a highly pathogenic complex which is able to invade and kill insect host within two days. Both bacteria and nematodes produce a variety of factors interacting with the insect immune system and help to overcome host defences. The tripartite model (Drosophila, nematodes, bacteria) was established and used to show an immune function for candidate genes using different Drosophila mutants or RNAi lines with defects in clotting or other branches of the immune system. Microarray analysis was used to compare gene expression of Drosophila larvae infected by the entomopathogenic nematode Heterorhabditis bacteriophora and its symbiotic bacterium Photorhabdus luminescens with non-infected larvae. The role of candidate genes, selected based on genomic comparison, in response towards nematobacterial complex was further evaluated by in vivo infection assays. We demonstrated an immune function during nematode infection for known clotting enzymes and substrates, recognition molecules and eicosanoids. In conclusion, we show that the Heterorhabditis/Photorhabdus infection model is suitable to identify regulators of innate immunity in insects.

Návaznosti

• GA17-03253S, projekt VaV: Název: Hormonální kontrola hmyzího obranného systému

Investor: Grantová agentura ČR, Hormonální kontrola hmyzího obranného systému