Amperometric immunosensor for diagnosis of albuminuria

a 2018

Amperometric immunosensor for diagnosis of albuminuria

MIKUŠOVÁ, Zuzana, Matěj PASTUCHA, Zdeněk FARKA, Veronika ČUNDERLOVÁ, Petr SKLÁDAL et. al.

Základní údaje

Originální název

Amperometric immunosensor for diagnosis of albuminuria

Autoři

MIKUŠOVÁ, Zuzana (703 Slovensko, domácí), Matěj PASTUCHA (203 Česká republika, domácí), Zdeněk FARKA (203 Česká republika, domácí), Veronika ČUNDERLOVÁ (203 Česká republika, domácí) a Petr SKLÁDAL (203 Česká republika, garant, domácí)

Vydání

CEITEC PhD and Postdoc Retreat, 2018

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14740/18:00102777

Organizační jednotka

Středoevropský technologický institut

ISBN

978-80-210-8941-9

Klíčová slova anglicky

Biosensor; Amperometry; Sandwich assay; Human serum albumin; Albuminuria

Štítky

rivok

Příznaky

Mezinárodní význam

Změněno: 11. 3. 2019 10:18, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Human serum albumin (HSA) is a plasma protein involved in transportation of ligands to specific targets. Its increased presence in urine (microalbuminuria) is considered as an indication of diabetic nephropathy. Currently, HSA is being typically detected by immunoassays such as ELISA. These methods provide high sensitivity; however, they are time consuming, complicated and expensive. One of the possibilities to over-come these disadvantages is to use electrochemical biosensors that offer operational simplicity, short analysis times and miniaturization possibilities for point-of-care diagnosis. The electrochemical biosensors are typically based on screen-printed electrodes (SPEs) due to their low cost, portability and mass production capabilities. We have developed an amperometric immunosensor for rapid diagnostics of albumi-nuria. Anti-HSA antibody was immobilized to the surface of SPEs that allowed specific capture of HSA. The detection was based on binding of secondary antibody conjugated with horseradish peroxidase (HRP), that provides oxidation of 3,3´,5,5´-tetramethylbenzidine (TMB) in presence of H2O2. Alternatively, Prussian blue catalytic nanoparticles were employed as convenient enzyme replacement. SPEs based on gold and carbon (including carbon nanotubes) working electrode were compared. This method has proved to be suitable for determination of clinically relevant concentrations of HSA in urine, optimization focused on better detection limit is in progress.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

MIKUŠOVÁ, Zuzana, Matěj PASTUCHA, Zdeněk FARKA, Veronika ČUNDERLOVÁ a Petr SKLÁDAL. Amperometric immunosensor for diagnosis of albuminuria. In CEITEC PhD and Postdoc Retreat. 2018. ISBN 978-80-210-8941-9.

@proceedings{1418321,
   author = {Mikušová, Zuzana and Pastucha, Matěj and Farka, Zdeněk and Čunderlová, Veronika and Skládal, Petr},
   booktitle = {CEITEC PhD and Postdoc Retreat},
   keywords = {Biosensor; Amperometry; Sandwich assay; Human serum albumin; Albuminuria},
   language = {eng},
   isbn = {978-80-210-8941-9},
   title = {Amperometric immunosensor for diagnosis of albuminuria},
   url = {http://phdretreat.ceitec.cz/download/174},
   year = {2018}
}

TY  - CONF
ID  - 1418321
AU  - Mikušová, Zuzana - Pastucha, Matěj - Farka, Zdeněk - Čunderlová, Veronika - Skládal, Petr
PY  - 2018
TI  - Amperometric immunosensor for diagnosis of albuminuria
SN  - 9788021089419
KW  - Biosensor
KW  - Amperometry
KW  - Sandwich assay
KW  - Human serum albumin
KW  - Albuminuria
UR  - http://phdretreat.ceitec.cz/download/174
N2  - Human serum albumin (HSA) is a plasma protein involved in transportation of ligands to specific targets. Its increased presence in urine (microalbuminuria) is considered as an indication of diabetic nephropathy. Currently, HSA is being typically detected by immunoassays such as ELISA. These methods provide high sensitivity; however, they are time consuming, complicated and expensive. One of the possibilities to over-come these disadvantages is to use electrochemical biosensors that offer operational simplicity, short analysis times and miniaturization possibilities for point-of-care diagnosis. The electrochemical biosensors are typically based on screen-printed electrodes (SPEs) due to their low cost, portability and mass production capabilities. We have developed an amperometric immunosensor for rapid diagnostics of albumi-nuria. Anti-HSA antibody was immobilized to the surface of SPEs that allowed specific capture of HSA. The detection was based on binding of secondary antibody conjugated with horseradish peroxidase (HRP), that provides oxidation of 3,3´,5,5´-tetramethylbenzidine (TMB) in presence of H2O2. Alternatively, Prussian blue catalytic nanoparticles were employed as convenient enzyme replacement. SPEs based on gold and carbon (including carbon nanotubes) working electrode were compared. This method has proved to be suitable for determination of clinically relevant concentrations of HSA in urine, optimization focused on better detection limit is in progress.
ER  -

MIKUŠOVÁ, Zuzana, Matěj PASTUCHA, Zdeněk FARKA, Veronika ČUNDERLOVÁ a Petr SKLÁDAL. Amperometric immunosensor for diagnosis of albuminuria. In \textit{CEITEC PhD and Postdoc Retreat}. 2018. ISBN~978-80-210-8941-9.

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