Další formáty:
BibTeX
LaTeX
RIS
@article{1419178,
author = {Priebojová, Jana and Hilscherová, Klára and Procházková, Tereza and Sychrová, Eliška and Smutná, Marie},
article_location = {San Diego (CA) USA},
article_number = {April},
doi = {http://dx.doi.org/10.1016/j.ecoenv.2017.12.048},
keywords = {Cyanobacteria; Retinoids; Retinoid-like activity; Extract; Exudates; ATRA},
language = {eng},
issn = {0147-6513},
journal = {Ecotoxicology and Environmental Safety},
title = {Intracellular and extracellular retinoid-like activity of widespread cyanobacterial species},
url = {https://www.sciencedirect.com/science/article/pii/S0147651317308825?via%3Dihub},
volume = {150},
year = {2018}
}
TY - JOUR
ID - 1419178
AU - Priebojová, Jana - Hilscherová, Klára - Procházková, Tereza - Sychrová, Eliška - Smutná, Marie
PY - 2018
TI - Intracellular and extracellular retinoid-like activity of widespread cyanobacterial species
JF - Ecotoxicology and Environmental Safety
VL - 150
IS - April
SP - 312-319
EP - 312-319
PB - ACADEMIC PRESS INC ELSEVIER SCIENCE
SN - 01476513
KW - Cyanobacteria
KW - Retinoids
KW - Retinoid-like activity
KW - Extract
KW - Exudates
KW - ATRA
UR - https://www.sciencedirect.com/science/article/pii/S0147651317308825?via%3Dihub
L2 - https://www.sciencedirect.com/science/article/pii/S0147651317308825?via%3Dihub
N2 - Cyanobacterial species produce wide range of bioactive compounds. This study characterized production of retinoid-like compounds with embryotoxic and teratogenic potential by commonly occurring cyanobacterial species with tendency to form massive water blooms. The major goal was to simultaneously assess the intracellular and extracellular retinoid-like activity from several independent cultivations of one coccal (Microcystis aeruginosa) and four filamentous cyanobacteria (Aphanizomenon gracile, Cylindrospermopsis raciborskii, Linutothrix redekeii, and Planktothrix agardhii) and characterize the variability in its production among cultivations. The retinoid-like activity was evaluated by in vitro assay along with chemical analyses of nine retinoids: all-trans retinoic acid (ATRA), 9-cis retinoic acid (9cis-RA), 13cis-RA, 13cis-RA methyl ester, 5,6 epoxy RA, 4keto-ATRA, 4keto-retinal, 4hydoxy-retinoic add (4OH-ATRA), retinal and retinol. The production of retinoid-like compounds was recalculated per volume, per biomass dry weight and per cell to provide relevant data for risk assessment in relation to occurrence of massive water blooms in the environment. Total produced retinoid-like activity of five selected species ranged from 170 to 25,600 ng ATRA-equivalents (REQ)/g dm corresponding to 0.001-0.392 ng REQ/10(6) cyanobacterial cells. Results from chemical analyses showed that all tested extracts contained 4keto-ATRA and retinal. All-trans retinoic acid, 9/13cis-retinoic acid and 5,6 epoxyretinoic acid were detected in most exudate and extract samples. The reported results of recalculated total retinoid-like activity enable potential predictions of its production by the studied species in water blooms of known cell densities relevant for risk assessment.
ER -
PRIEBOJOVÁ, Jana, Klára HILSCHEROVÁ, Tereza PROCHÁZKOVÁ, Eliška SYCHROVÁ a Marie SMUTNÁ. Intracellular and extracellular retinoid-like activity of widespread cyanobacterial species. \textit{Ecotoxicology and Environmental Safety}. San Diego (CA) USA: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2018, roč.~150, April, s.~312-319. ISSN~0147-6513. Dostupné z: https://dx.doi.org/10.1016/j.ecoenv.2017.12.048.
|
