Estrogenic activity and contributing compounds in stagnant
water bodies with massive occurrence of phytoplankton

J 2018

Estrogenic activity and contributing compounds in stagnant water bodies with massive occurrence of phytoplankton

PROCHÁZKOVÁ, Tereza; Eliška SYCHROVÁ; Jaroslava VEČERKOVÁ; Barbora JAVŮRKOVÁ; Alena OTOUPALÍKOVÁ et al.

Základní údaje

Originální název

Estrogenic activity and contributing compounds in stagnant water bodies with massive occurrence of phytoplankton

Autoři

PROCHÁZKOVÁ, Tereza; Eliška SYCHROVÁ ; Jaroslava VEČERKOVÁ; Barbora JAVŮRKOVÁ; Alena OTOUPALÍKOVÁ; Marek PERNICA; Zdeněk ŠIMEK; Marie SMUTNÁ; O. LEPSOVA-SKACELOVA a Klára HILSCHEROVÁ

Vydání

Water Research, OXFORD, PERGAMON-ELSEVIER SCIENCE LTD, 2018, 0043-1354

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10503 Water resources

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.913

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/18:00100973

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Estrogenicity; Surface water; Flavonoids; Alkylphenols; Estrogens

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 6. 2018 10:27, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Stagnant water bodies have generally received little attention regarding the presence of endocrine disruptive compounds, although they can integrate diverse pollutants from multiple different sources. Many compounds of anthropogenic as well as natural origin can contribute to the overall estrogenicity of surface waters and some of them can exhibit adverse effects on aquatic biota even in very low concentrations. This study focused on freshwater ponds and reservoirs affected by water blooms and determined the estrogenic activity of water by in vitro bioassay as well as concentrations of several important groups of estrogenic compounds (estrogenic hormones, alkylphenols, and phytoestrogens) by LC-MS/MS analyses. Estrogenic hormones were found at concentrations up to 7.1 ng.L-1, similarly to flavonoids, whose concentrations did not exceed 12.5 ng.L-1. Among alkylphenols, only bisphenol A and 4-tert-octylphenol were detected in levels reaching 100 ng.L-1 at maximum. Estrogenic activity of water samples varied from below the quantification limit to 1.95 ng.L-1. There does not seem to be any general causal link of the massive phytoplankton occurrence with the estrogenicity of water or concentration of phytoestrogens, since they showed no direct relationship with the phytoplankton abundance or composition across sites. The contribution of the analysed compounds to the estrogenic activity was calculated in three scenarios. In minimum scenario, just the compounds above quantification limit (LOQ) were taken into account and for most samples, only minor part (<6%) of the biological activity could be explained. In the mean and maximum scenarios, we included also compounds below LOQ into the calculations at the level of LOQ/2 and LOQ respectively. In these cases, a considerable part of the estrogenic activity could be attributed to the possible presence of steroid estrogens below LOQ However, for the samples with estrogenic activity greater than 1 ng.L-1, more than 50% of the estrogenic activity remained unexplained even in the maximum scenario. Probably other compounds or possible interactions between individual substances cause the estrogenic activity in these types of water bodies and in this case, the results of LC-MS/MS analyses cannot sufficiently predict the biological effects. A complex approach including bioassays is needed when assessing the estrogenicity of these types of surface waters.

Návaznosti

CZ.02.1.01/0.0/0.0/16_013/0001761, interní kód MU

Název: RECETOX RI - OP VVV (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, RECETOX RI - OP VVV, PO 1 Posilování kapacit pro kvalitní výzkum

GA13-20357S, projekt VaV

Název: Studium distribuce steroidních látek ve složkách pevných environmentálních matric.

Investor: Grantová agentura ČR, Studium distribuce steroidních látek ve složkách pevných environmentálních matric

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

603437, interní kód MU

Název: SOLUTIONS - Solutions for present and future emerging pollutants in land and water resources management (Akronym: SOLUTIONS)

Investor: Evropská unie, SOLUTIONS - Solutions for present and future emerging pollutants in land and water resources management, Spolupráce

Citovat

PROCHÁZKOVÁ, Tereza; Eliška SYCHROVÁ; Jaroslava VEČERKOVÁ; Barbora JAVŮRKOVÁ; Alena OTOUPALÍKOVÁ; Marek PERNICA; Zdeněk ŠIMEK; Marie SMUTNÁ; O. LEPSOVA-SKACELOVA a Klára HILSCHEROVÁ. Estrogenic activity and contributing compounds in stagnant water bodies with massive occurrence of phytoplankton. Water Research. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2018, roč. 136, June, s. 12-21. ISSN 0043-1354. Dostupné z: https://doi.org/10.1016/j.watres.2018.02.040.

@article{1419294,
   author = {Procházková, Tereza and Sychrová, Eliška and Večerková, Jaroslava and Javůrková, Barbora and Otoupalíková, Alena and Pernica, Marek and Šimek, Zdeněk and Smutná, Marie and LepsovaandSkacelova, O. and Hilscherová, Klára},
   article_location = {OXFORD},
   article_number = {June},
   doi = {https://doi.org/10.1016/j.watres.2018.02.040},
   keywords = {Estrogenicity; Surface water; Flavonoids; Alkylphenols; Estrogens},
   language = {eng},
   issn = {0043-1354},
   journal = {Water Research},
   title = {Estrogenic activity and contributing compounds in stagnant water bodies with massive occurrence of phytoplankton},
   url = {https://www.sciencedirect.com/science/article/pii/S0043135418301477?via%3Dihub},
   volume = {136},
   year = {2018}
}

TY  - JOUR
ID  - 1419294
AU  - Procházková, Tereza - Sychrová, Eliška - Večerková, Jaroslava - Javůrková, Barbora - Otoupalíková, Alena - Pernica, Marek - Šimek, Zdeněk - Smutná, Marie - Lepsova-Skacelova, O. - Hilscherová, Klára
PY  - 2018
TI  - Estrogenic activity and contributing compounds in stagnant water bodies with massive occurrence of phytoplankton
JF  - Water Research
VL  - 136
IS  - June
SP  - 12-21
EP  - 12-21
PB  - PERGAMON-ELSEVIER SCIENCE LTD
SN  - 00431354
KW  - Estrogenicity
KW  - Surface water
KW  - Flavonoids
KW  - Alkylphenols
KW  - Estrogens
UR  - https://www.sciencedirect.com/science/article/pii/S0043135418301477?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0043135418301477?via%3Dihub
N2  - Stagnant water bodies have generally received little attention regarding the presence of endocrine disruptive compounds, although they can integrate diverse pollutants from multiple different sources. Many compounds of anthropogenic as well as natural origin can contribute to the overall estrogenicity of surface waters and some of them can exhibit adverse effects on aquatic biota even in very low concentrations. This study focused on freshwater ponds and reservoirs affected by water blooms and determined the estrogenic activity of water by in vitro bioassay as well as concentrations of several important groups of estrogenic compounds (estrogenic hormones, alkylphenols, and phytoestrogens) by LC-MS/MS analyses. Estrogenic hormones were found at concentrations up to 7.1 ng.L-1, similarly to flavonoids, whose concentrations did not exceed 12.5 ng.L-1. Among alkylphenols, only bisphenol A and 4-tert-octylphenol were detected in levels reaching 100 ng.L-1 at maximum. Estrogenic activity of water samples varied from below the quantification limit to 1.95 ng.L-1. There does not seem to be any general causal link of the massive phytoplankton occurrence with the estrogenicity of water or concentration of phytoestrogens, since they showed no direct relationship with the phytoplankton abundance or composition across sites. The contribution of the analysed compounds to the estrogenic activity was calculated in three scenarios. In minimum scenario, just the compounds above quantification limit (LOQ) were taken into account and for most samples, only minor part (<6%) of the biological activity could be explained. In the mean and maximum scenarios, we included also compounds below LOQ into the calculations at the level of LOQ/2 and LOQ respectively. In these cases, a considerable part of the estrogenic activity could be attributed to the possible presence of steroid estrogens below LOQ However, for the samples with estrogenic activity greater than 1 ng.L-1, more than 50% of the estrogenic activity remained unexplained even in the maximum scenario. Probably other compounds or possible interactions between individual substances cause the estrogenic activity in these types of water bodies and in this case, the results of LC-MS/MS analyses cannot sufficiently predict the biological effects. A complex approach including bioassays is needed when assessing the estrogenicity of these types of surface waters.
ER  -

PROCHÁZKOVÁ, Tereza; Eliška SYCHROVÁ; Jaroslava VEČERKOVÁ; Barbora JAVŮRKOVÁ; Alena OTOUPALÍKOVÁ; Marek PERNICA; Zdeněk ŠIMEK; Marie SMUTNÁ; O. LEPSOVA-SKACELOVA a Klára HILSCHEROVÁ. Estrogenic activity and contributing compounds in stagnant water bodies with massive occurrence of phytoplankton. \textit{Water Research}. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2018, roč.~136, June, s.~12-21. ISSN~0043-1354. Dostupné z: https://doi.org/10.1016/j.watres.2018.02.040.

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