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@article{1419753,
author = {Mytsyk, Yulian and Dosenko, Victor and Borys, Yuriy and Kucher, Askold and Gazdikova, Katarina and Busselberg, Dietrich and Caprnda, Martin and Kružliak, Peter and Farooqi, Ammad Ahmad and Lubov, Manyuk},
article_location = {Dordrecht},
article_number = {5},
doi = {http://dx.doi.org/10.1007/s11255-018-1841-x},
keywords = {Renal cell carcinoma; MiRNA-15a; Biomarker; Diagnosis; Molecular marker},
language = {eng},
issn = {0301-1623},
journal = {International Urology and Nephrology},
title = {MicroRNA-15a expression measured in urine samples as a potential biomarker of renal cell carcinoma},
volume = {50},
year = {2018}
}
TY - JOUR
ID - 1419753
AU - Mytsyk, Yulian - Dosenko, Victor - Borys, Yuriy - Kucher, Askold - Gazdikova, Katarina - Busselberg, Dietrich - Caprnda, Martin - Kružliak, Peter - Farooqi, Ammad Ahmad - Lubov, Manyuk
PY - 2018
TI - MicroRNA-15a expression measured in urine samples as a potential biomarker of renal cell carcinoma
JF - International Urology and Nephrology
VL - 50
IS - 5
SP - 851-859
EP - 851-859
PB - Springer
SN - 03011623
KW - Renal cell carcinoma
KW - MiRNA-15a
KW - Biomarker
KW - Diagnosis
KW - Molecular marker
N2 - Currently, there is no accurate diagnostic molecular biomarker for renal cell carcinoma (RCC). The aim of this study was to assess the expression of microRNA-15a (miR-15a) in urine of patients with RCC and to evaluate its potential as a diagnostic molecular biomarker. In total, 67 patients with solid renal tumors were enrolled: clear-cell RCC (ccRCC, n = 22), papillary RCC (pRCC, n = 16), chromophobe RCC (chRCC, n = 14), oncocytoma (n = 8), papillary adenoma (n = 2) and angiomyolipoma (n = 5). MiRNA-15a expression levels measurement in urine were performed using qPCR. Urine of 15 healthy volunteers without kidney pathology was used as control. We observed a difference in mean miR-15a expression values in groups of pre-operative patients with RCC, benign renal tumors and healthy persons (2.50E-01 +/- 2.72E-01 vs 1.32E-03 +/- 3.90E-03 vs 3.36E-07 +/- 1.04E-07 RFU, respectively, p < 0.01). There was no difference in miR-15a expression between ccRCC, pRCC and chRCC (p > 0.05). Direct association between RCC size and miR-15a expression values was obtained (Pearson correlation coefficient-0.873). On the 8th day after nephrectomy, mean expression value in patients with RCC decreased by 99.53% (p < 0.01). MiR-15a expression differentiated RCC from benign renal tumors with 98.1% specificity, 100% sensitivity at a cut-off value of 5.00E-06 RFU, with AUC-0.955. MiR-15a expression measured in urine may be used as diagnostic molecular biomarker for RCC.
ER -
MYTSYK, Yulian, Victor DOSENKO, Yuriy BORYS, Askold KUCHER, Katarina GAZDIKOVA, Dietrich BUSSELBERG, Martin CAPRNDA, Peter KRUŽLIAK, Ammad Ahmad FAROOQI a Manyuk LUBOV. MicroRNA-15a expression measured in urine samples as a potential biomarker of renal cell carcinoma. \textit{International Urology and Nephrology}. Dordrecht: Springer, 2018, roč.~50, č.~5, s.~851-859. ISSN~0301-1623. Dostupné z: https://dx.doi.org/10.1007/s11255-018-1841-x.
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