MicroRNA-15a expression measured in urine samples as a
potential biomarker of renal cell carcinoma

J 2018

MicroRNA-15a expression measured in urine samples as a potential biomarker of renal cell carcinoma

MYTSYK, Yulian; Victor DOSENKO; Yuriy BORYS; Askold KUCHER; Katarina GAZDIKOVA et al.

Základní údaje

Originální název

MicroRNA-15a expression measured in urine samples as a potential biomarker of renal cell carcinoma

Autoři

MYTSYK, Yulian; Victor DOSENKO; Yuriy BORYS; Askold KUCHER; Katarina GAZDIKOVA; Dietrich BUSSELBERG; Martin CAPRNDA; Peter KRUŽLIAK; Ammad Ahmad FAROOQI a Manyuk LUBOV

Vydání

International Urology and Nephrology, Dordrecht, Springer, 2018, 0301-1623

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30217 Urology and nephrology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.596

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/18:00102916

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Renal cell carcinoma; MiRNA-15a; Biomarker; Diagnosis; Molecular marker

Štítky

14110121, EL OK, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 2. 2019 16:07, Soňa Böhmová

Anotace

V originále

Currently, there is no accurate diagnostic molecular biomarker for renal cell carcinoma (RCC). The aim of this study was to assess the expression of microRNA-15a (miR-15a) in urine of patients with RCC and to evaluate its potential as a diagnostic molecular biomarker. In total, 67 patients with solid renal tumors were enrolled: clear-cell RCC (ccRCC, n = 22), papillary RCC (pRCC, n = 16), chromophobe RCC (chRCC, n = 14), oncocytoma (n = 8), papillary adenoma (n = 2) and angiomyolipoma (n = 5). MiRNA-15a expression levels measurement in urine were performed using qPCR. Urine of 15 healthy volunteers without kidney pathology was used as control. We observed a difference in mean miR-15a expression values in groups of pre-operative patients with RCC, benign renal tumors and healthy persons (2.50E-01 +/- 2.72E-01 vs 1.32E-03 +/- 3.90E-03 vs 3.36E-07 +/- 1.04E-07 RFU, respectively, p < 0.01). There was no difference in miR-15a expression between ccRCC, pRCC and chRCC (p > 0.05). Direct association between RCC size and miR-15a expression values was obtained (Pearson correlation coefficient-0.873). On the 8th day after nephrectomy, mean expression value in patients with RCC decreased by 99.53% (p < 0.01). MiR-15a expression differentiated RCC from benign renal tumors with 98.1% specificity, 100% sensitivity at a cut-off value of 5.00E-06 RFU, with AUC-0.955. MiR-15a expression measured in urine may be used as diagnostic molecular biomarker for RCC.

Citovat

MYTSYK, Yulian; Victor DOSENKO; Yuriy BORYS; Askold KUCHER; Katarina GAZDIKOVA; Dietrich BUSSELBERG; Martin CAPRNDA; Peter KRUŽLIAK; Ammad Ahmad FAROOQI a Manyuk LUBOV. MicroRNA-15a expression measured in urine samples as a potential biomarker of renal cell carcinoma. International Urology and Nephrology. Dordrecht: Springer, 2018, roč. 50, č. 5, s. 851-859. ISSN 0301-1623. Dostupné z: https://doi.org/10.1007/s11255-018-1841-x.

@article{1419753,
   author = {Mytsyk, Yulian and Dosenko, Victor and Borys, Yuriy and Kucher, Askold and Gazdikova, Katarina and Busselberg, Dietrich and Caprnda, Martin and Kružliak, Peter and Farooqi, Ammad Ahmad and Lubov, Manyuk},
   article_location = {Dordrecht},
   article_number = {5},
   doi = {https://doi.org/10.1007/s11255-018-1841-x},
   keywords = {Renal cell carcinoma; MiRNA-15a; Biomarker; Diagnosis; Molecular marker},
   language = {eng},
   issn = {0301-1623},
   journal = {International Urology and Nephrology},
   title = {MicroRNA-15a expression measured in urine samples as a potential biomarker of renal cell carcinoma},
   volume = {50},
   year = {2018}
}

TY  - JOUR
ID  - 1419753
AU  - Mytsyk, Yulian - Dosenko, Victor - Borys, Yuriy - Kucher, Askold - Gazdikova, Katarina - Busselberg, Dietrich - Caprnda, Martin - Kružliak, Peter - Farooqi, Ammad Ahmad - Lubov, Manyuk
PY  - 2018
TI  - MicroRNA-15a expression measured in urine samples as a potential biomarker of renal cell carcinoma
JF  - International Urology and Nephrology
VL  - 50
IS  - 5
SP  - 851-859
EP  - 851-859
PB  - Springer
SN  - 03011623
KW  - Renal cell carcinoma
KW  - MiRNA-15a
KW  - Biomarker
KW  - Diagnosis
KW  - Molecular marker
N2  - Currently, there is no accurate diagnostic molecular biomarker for renal cell carcinoma (RCC). The aim of this study was to assess the expression of microRNA-15a (miR-15a) in urine of patients with RCC and to evaluate its potential as a diagnostic molecular biomarker. In total, 67 patients with solid renal tumors were enrolled: clear-cell RCC (ccRCC, n = 22), papillary RCC (pRCC, n = 16), chromophobe RCC (chRCC, n = 14), oncocytoma (n = 8), papillary adenoma (n = 2) and angiomyolipoma (n = 5). MiRNA-15a expression levels measurement in urine were performed using qPCR. Urine of 15 healthy volunteers without kidney pathology was used as control. We observed a difference in mean miR-15a expression values in groups of pre-operative patients with RCC, benign renal tumors and healthy persons (2.50E-01 +/- 2.72E-01 vs 1.32E-03 +/- 3.90E-03 vs 3.36E-07 +/- 1.04E-07 RFU, respectively, p < 0.01). There was no difference in miR-15a expression between ccRCC, pRCC and chRCC (p > 0.05). Direct association between RCC size and miR-15a expression values was obtained (Pearson correlation coefficient-0.873). On the 8th day after nephrectomy, mean expression value in patients with RCC decreased by 99.53% (p < 0.01). MiR-15a expression differentiated RCC from benign renal tumors with 98.1% specificity, 100% sensitivity at a cut-off value of 5.00E-06 RFU, with AUC-0.955. MiR-15a expression measured in urine may be used as diagnostic molecular biomarker for RCC.
ER  -

MYTSYK, Yulian; Victor DOSENKO; Yuriy BORYS; Askold KUCHER; Katarina GAZDIKOVA; Dietrich BUSSELBERG; Martin CAPRNDA; Peter KRUŽLIAK; Ammad Ahmad FAROOQI a Manyuk LUBOV. MicroRNA-15a expression measured in urine samples as a potential biomarker of renal cell carcinoma. \textit{International Urology and Nephrology}. Dordrecht: Springer, 2018, roč.~50, č.~5, s.~851-859. ISSN~0301-1623. Dostupné z: https://doi.org/10.1007/s11255-018-1841-x.

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