Methods for assessing the sensitivity of intestinal lactic acid
bacteria to hydrogen sulfide

a 2018

Methods for assessing the sensitivity of intestinal lactic acid bacteria to hydrogen sulfide

KOTRSOVÁ, Věra a Ivan KUSHKEVYCH

Základní údaje

Originální název

Methods for assessing the sensitivity of intestinal lactic acid bacteria to hydrogen sulfide

Název česky

Metody pro hodnocení toxicity sirovodíku na střevní mléčné bakterie

Autoři

KOTRSOVÁ, Věra a Ivan KUSHKEVYCH

Vydání

Květinův den, 2018

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10606 Microbiology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/18:00102930

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Lactic acid bacteria; hydrogen sulfide; sulfate reducing bacteria; intestine

Změněno: 5. 6. 2018 14:26, Mgr. Věra Kotrsová

Anotace

V originále

Lactic acid bacteria are undoubtedly important part of the intestinal microbiome including especially Lactobacillus, Streptococcus, and Bifidobacterium genera. Sulfate-reducing bacteria (SRB), including Desulfovibrio species, are presented in the intestine consuming the sulfate or sulfite ions as a terminal electron acceptor while sulfide is produced. According to acidic intestinal environment, sulfide is hydrolyzed to hydrogen sulfide. There is an overproduction of hydrogen sulfide in patients suffering from ulcerative colitis. Many research papers show evidence that higher occurrence of SRB and their hydrogen sulfide overproduction is one of the factors in ulcerative colitis. The number of lactic acid bacteria during colonic inflammation decrease, while the number of SRB and their metabolites (hydrogen sulfide and acetate) increase. The effect of these metabolites on lactic acid bacteria has never been explored. Lactic acid bacteria play a fundamental role in the intestinal microbiome, and therefore it is important to investigate this issue more deeply. This study is focused on the effect of hydrogen sulfide on the intestinal lactic acid bacteria as well as to test various methods that could be used for its evaluation. Spectrophotometric, colorimetric, agar plate methods and combination of these methods were tested. Samples of lactic acid bacteria were isolated from colon affected by ulcerative colitis, probiotic pills and from milk products. The obtained results proved that lactic bacteria show high sensitivity to hydrogen sulfide. Thousands of people from all over the world are affected by inflammatory bowel disease every year. The results gained from this research can help to improve the stability of intestinal microbiome, improve the treatment of bowel inflammation, or prevent bowel disease altogether.

Citovat

KOTRSOVÁ, Věra a Ivan KUSHKEVYCH. Methods for assessing the sensitivity of intestinal lactic acid bacteria to hydrogen sulfide. In Květinův den. 2018.

@proceedings{1419806,
   author = {Kotrsová, Věra and Kushkevych, Ivan},
   booktitle = {Květinův den},
   keywords = {Lactic acid bacteria; hydrogen sulfide; sulfate reducing bacteria; intestine},
   language = {eng},
   title = {Methods for assessing the sensitivity of intestinal lactic acid bacteria to hydrogen sulfide},
   year = {2018}
}

TY  - CONF
ID  - 1419806
AU  - Kotrsová, Věra - Kushkevych, Ivan
PY  - 2018
TI  - Methods for assessing the sensitivity of intestinal lactic acid bacteria to hydrogen sulfide
KW  - Lactic acid bacteria
KW  - hydrogen sulfide
KW  - sulfate reducing bacteria
KW  - intestine
N2  - Lactic acid bacteria are undoubtedly important part of the intestinal microbiome including especially Lactobacillus, Streptococcus, and Bifidobacterium genera. Sulfate-reducing bacteria (SRB), including Desulfovibrio species, are presented in the intestine consuming the sulfate or sulfite ions as a terminal electron acceptor while sulfide is produced. According to acidic intestinal environment, sulfide is hydrolyzed to hydrogen sulfide. There is an overproduction of hydrogen sulfide in patients suffering from ulcerative colitis. Many research papers show evidence that higher occurrence of SRB and their hydrogen sulfide overproduction is one of the factors in ulcerative colitis. The number of lactic acid bacteria during colonic inflammation decrease, while the number of SRB and their metabolites (hydrogen sulfide and acetate) increase. The effect of these metabolites on lactic acid bacteria has never been explored. Lactic acid bacteria play a fundamental role in the intestinal microbiome, and therefore it is important to investigate this issue more deeply. This study is focused on the effect of hydrogen sulfide on the intestinal lactic acid bacteria as well as to test various methods that could be used for its evaluation. Spectrophotometric, colorimetric, agar plate methods and combination of these methods were tested. Samples of lactic acid bacteria were isolated from colon affected by ulcerative colitis, probiotic pills and from milk products. The obtained results proved that lactic bacteria show high sensitivity to hydrogen sulfide. Thousands of people from all over the world are affected by inflammatory bowel disease every year. The results gained from this research can help to improve the stability of intestinal microbiome, improve the treatment of bowel inflammation, or prevent bowel disease altogether.
ER  -

KOTRSOVÁ, Věra a Ivan KUSHKEVYCH. Methods for assessing the sensitivity of intestinal lactic acid bacteria to hydrogen sulfide. In \textit{Květinův den}. 2018.

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