A monolithic lipase reactor for biodiesel production by
transesterification of triacylglycerides into fatty acid methyl
esters

J 2012

A monolithic lipase reactor for biodiesel production by transesterification of triacylglycerides into fatty acid methyl esters

URBAN, Jiří, Frantisek SVEC a Jean M. J. FRÉCHET

Základní údaje

Originální název

A monolithic lipase reactor for biodiesel production by transesterification of triacylglycerides into fatty acid methyl esters

Autoři

URBAN, Jiří, Frantisek SVEC a Jean M. J. FRÉCHET

Vydání

Biotechnology and Bioengineering, New York, Wiley, 2012, 0006-3592

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.648

DOI

http://dx.doi.org/10.1002/bit.23326

UT WoS

000298020700008

Klíčová slova anglicky

biodiesel; transesterification; lipase; soybean oil; fatty acid methyl esters; polymer monolith; design of experiments; response surface methodology; immobilization

Změněno: 12. 3. 2021 13:18, doc. RNDr. Jiří Urban, Ph.D.

Anotace

V originále

An enzymatic reactor with lipase immobilized on a monolithic polymer support has been prepared and used to catalyze the transesterification of triacylglycerides into the fatty acid methyl esters commonly used for biodiesel. A design of experiments procedure was used to optimize the monolithic reactor with variables including control of the surface polarity of the monolith via variations in the length of the hydrocarbon chain in alkyl methacrylate monomer, time of grafting of 1-vinyl-4,4-dimethylazlactone used to activate the monolith, and time used for the immobilization of porcine lipase. Optimal conditions involved the use of a poly(stearyl methacrylate-co-ethylene dimethacrylate) monolith, grafted first with vinylazlactone, then treated with lipase for 2?h to carry out the immobilization of the enzyme. Best conditions for the transesterification of glyceryl tributyrate included a temperature of 37 degrees C and a 10?min residence time of the substrate in the bioreactor. The reactor did not lose its activity even after pumping through it a solution of substrate equaling 1,000 reactor volumes. This enzymatic reactor was also used for the transesterification of triacylglycerides from soybean oil to fatty acid methyl esters thus demonstrating the ability of the reactor to produce biodiesel. Biotechnol. Bioeng. 2012; 109:371380. (c) 2011 Wiley Periodicals, Inc.

Citovat

URBAN, Jiří, Frantisek SVEC a Jean M. J. FRÉCHET. A monolithic lipase reactor for biodiesel production by transesterification of triacylglycerides into fatty acid methyl esters. Biotechnology and Bioengineering. New York: Wiley, 2012, roč. 109, č. 2, s. 371-380. ISSN 0006-3592. Dostupné z: https://dx.doi.org/10.1002/bit.23326.

@article{1419808,
   author = {Urban, Jiří and Svec, Frantisek and Fréchet, Jean M. J.},
   article_location = {New York},
   article_number = {2},
   doi = {http://dx.doi.org/10.1002/bit.23326},
   keywords = {biodiesel; transesterification; lipase; soybean oil; fatty acid methyl esters; polymer monolith; design of experiments; response surface methodology; immobilization},
   language = {eng},
   issn = {0006-3592},
   journal = {Biotechnology and Bioengineering},
   title = {A monolithic lipase reactor for biodiesel production by transesterification of triacylglycerides into fatty acid methyl esters},
   volume = {109},
   year = {2012}
}

TY  - JOUR
ID  - 1419808
AU  - Urban, Jiří - Svec, Frantisek - Fréchet, Jean M. J.
PY  - 2012
TI  - A monolithic lipase reactor for biodiesel production by transesterification of triacylglycerides into fatty acid methyl esters
JF  - Biotechnology and Bioengineering
VL  - 109
IS  - 2
SP  - 371-380
EP  - 371-380
PB  - Wiley
SN  - 00063592
KW  - biodiesel
KW  - transesterification
KW  - lipase
KW  - soybean oil
KW  - fatty acid methyl esters
KW  - polymer monolith
KW  - design of experiments
KW  - response surface methodology
KW  - immobilization
N2  - An enzymatic reactor with lipase immobilized on a monolithic polymer support has been prepared and used to catalyze the transesterification of triacylglycerides into the fatty acid methyl esters commonly used for biodiesel. A design of experiments procedure was used to optimize the monolithic reactor with variables including control of the surface polarity of the monolith via variations in the length of the hydrocarbon chain in alkyl methacrylate monomer, time of grafting of 1-vinyl-4,4-dimethylazlactone used to activate the monolith, and time used for the immobilization of porcine lipase. Optimal conditions involved the use of a poly(stearyl methacrylate-co-ethylene dimethacrylate) monolith, grafted first with vinylazlactone, then treated with lipase for 2?h to carry out the immobilization of the enzyme. Best conditions for the transesterification of glyceryl tributyrate included a temperature of 37 degrees C and a 10?min residence time of the substrate in the bioreactor. The reactor did not lose its activity even after pumping through it a solution of substrate equaling 1,000 reactor volumes. This enzymatic reactor was also used for the transesterification of triacylglycerides from soybean oil to fatty acid methyl esters thus demonstrating the ability of the reactor to produce biodiesel. Biotechnol. Bioeng. 2012; 109:371380. (c) 2011 Wiley Periodicals, Inc.
ER  -

URBAN, Jiří, Frantisek SVEC a Jean M. J. FRÉCHET. A monolithic lipase reactor for biodiesel production by transesterification of triacylglycerides into fatty acid methyl esters. \textit{Biotechnology and Bioengineering}. New York: Wiley, 2012, roč.~109, č.~2, s.~371-380. ISSN~0006-3592. Dostupné z: https://dx.doi.org/10.1002/bit.23326.

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