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@proceedings{1422221,
author = {Kučera, Josef and Peš, Ondřej and Voráčová, Ivona and Táborský, Petr},
booktitle = {16th Czech - Slovak Spectroscopic Conference 2018},
keywords = {Fluorescence; enhancement; quenching of luminescence; deuterated water; anthracyclines; HPLC; chromatography; capillary electrophoresis; CE-LIF},
language = {eng},
isbn = {978-80-88195-06-1},
title = {Enhancement of sensitivity in separation techniques with fluorescence detection using deuterated water},
year = {2018}
}
TY - CONF
ID - 1422221
AU - Kučera, Josef - Peš, Ondřej - Voráčová, Ivona - Táborský, Petr
PY - 2018
TI - Enhancement of sensitivity in separation techniques with fluorescence detection using deuterated water
SN - 9788088195061
KW - Fluorescence
KW - enhancement
KW - quenching of luminescence
KW - deuterated water
KW - anthracyclines
KW - HPLC
KW - chromatography
KW - capillary electrophoresis
KW - CE-LIF
N2 - Fluorescent molecules with emission maxima above 500 nm are often dynamically quenched by OH oscillators present in water molecules. We have changed H2O for D2O to obtain higher fluorescence intensity signal. Our experiments carried out on spectrofluorometer have proved that anthracyclines can achieve about 300 % higher response in D2O as solvent. The affect can be employed in various analytical methods including HPLC and capillary electrophoresis coupled to fluorescence detection.
ER -
KUČERA, Josef, Ondřej PEŠ, Ivona VORÁČOVÁ a Petr TÁBORSKÝ. Enhancement of sensitivity in separation techniques with fluorescence detection using deuterated water. In \textit{16th Czech - Slovak Spectroscopic Conference 2018}. 2018. ISBN~978-80-88195-06-1.
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