Mobile toilets chemicals: its influence on activated sludge
microorganisms

a 2018

Mobile toilets chemicals: its influence on activated sludge microorganisms

VÍTĚZOVÁ, Monika; Markéta KOPECKÁ; Tomáš VÍTĚZ a Tereza DOKULILOVÁ

Základní údaje

Originální název

Mobile toilets chemicals: its influence on activated sludge microorganisms

Autoři

VÍTĚZOVÁ, Monika (203 Česká republika, garant, domácí); Markéta KOPECKÁ (203 Česká republika, domácí); Tomáš VÍTĚZ (203 Česká republika) a Tereza DOKULILOVÁ

Vydání

4th International Conference on Pollution Control and Sustainable Environment, 2018

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10606 Microbiology

Stát vydavatele

Itálie

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/18:00103290

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

microbial respiration; activated sludge; wastewater treatment

Příznaky

Mezinárodní význam

Změněno: 3. 8. 2018 13:26, doc. Mgr. Monika Vítězová, Ph.D.

Anotace

V originále

Our work focuses on the wastewater treatment process, especially on the activated sludge. The activated sludge is composed of varied groups of microorganisms which play the crucial role in the removal of the organic matter and nitrogenous substances from the wastewater. In the previous study we found out that the chemical substances used regularly as a toilet chemistry negatively influence respirometric activity of the activated sludge. Regarding this fact, we decided to isolate different bacterial strains from the activated sludge to study the influence of the chemical substances on bacterial growth characteristics and mainly on the length of the lag phase. Lag phase is defined as the period where the individual bacteria are maturing and not yet able to divide. They adapt themselves to the new growth conditions. We isolated three bacterial strains from the activated sludge obtained from the municipal wastewater treatment plant in Modřice, Czech Republic (513.000 PE). The bacterial strains were cultivated on Nitrate Broth medium and on the basis of morphological features and Sanger´s sequencing of the DNA we have determined them as Paracoccus aminophilus, Ralstonia picketti and Psychrobacter sp. The growth of these bacterial strains was measured by the microplate reader Tecan SunriseTM (Tecan Trading AG, Switzerland). The samples examined contained inoculum of the strains mentioned above (P. aminophilus, R. picketti and Psychrobacter sp.) and the pure chemical substances in the appropriate concentrations. The results were evaluated by equations of bacterial growth. The lenght of the lag phase differs depending on the nature of the chemical substances. Bronopol, a substance frequently used in mobile toilets, prolongs the lag phase very significantly. On the other hand, Limonen or Citric Acid shorten the time of bacterial adaptation. The retention time of wastewater in the activation tank lies usually in the range of 6 to 8 hours. Regarding the extended lag phase the bacterial metabolism is insufficient for the properly treated wastewater.

Návaznosti

MUNI/A/1080/2017, interní kód MU

Název: Podpora výzkumné činnosti studentů Mikrobiologie a molekulární biotechnologie 2

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů Mikrobiologie a molekulární biotechnologie 2, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

VÍTĚZOVÁ, Monika; Markéta KOPECKÁ; Tomáš VÍTĚZ a Tereza DOKULILOVÁ. Mobile toilets chemicals: its influence on activated sludge microorganisms. In 4th International Conference on Pollution Control and Sustainable Environment. 2018.

@proceedings{1423786,
   author = {Vítězová, Monika and Kopecká, Markéta and Vítěz, Tomáš and Dokulilová, Tereza},
   booktitle = {4th International Conference on Pollution Control and Sustainable Environment},
   keywords = {microbial respiration; activated sludge; wastewater treatment},
   language = {eng},
   title = {Mobile toilets chemicals: its influence on activated sludge microorganisms},
   year = {2018}
}

TY  - CONF
ID  - 1423786
AU  - Vítězová, Monika - Kopecká, Markéta - Vítěz, Tomáš - Dokulilová, Tereza
PY  - 2018
TI  - Mobile toilets chemicals: its influence on activated sludge microorganisms
KW  - microbial respiration
KW  - activated sludge
KW  - wastewater treatment
N2  - Our work focuses on the wastewater treatment process, especially on the activated sludge. The activated sludge is composed of varied groups of microorganisms which play the crucial role in the removal of the organic matter and nitrogenous substances from the wastewater. In the previous study we found out that the chemical substances used regularly as a toilet chemistry negatively influence respirometric activity of the activated sludge. Regarding this fact, we decided to isolate different bacterial strains from the activated sludge to study the influence of the chemical substances on bacterial growth characteristics and mainly on the length of the lag phase. Lag phase is defined as the period where the individual bacteria are maturing and not yet able to divide. They adapt themselves to the new growth conditions. We isolated three bacterial strains from the activated sludge obtained from the municipal wastewater treatment plant in Modřice, Czech Republic (513.000 PE). The bacterial strains were cultivated on Nitrate Broth medium and on the basis of morphological features and Sanger´s sequencing of the DNA we have determined them as Paracoccus aminophilus, Ralstonia picketti and Psychrobacter sp. The growth of these bacterial strains was measured by the microplate reader Tecan SunriseTM (Tecan Trading AG, Switzerland). The samples examined contained inoculum of the strains mentioned above (P. aminophilus, R. picketti and Psychrobacter sp.) and the pure chemical substances in the appropriate concentrations. The results were evaluated by equations of bacterial growth. The lenght of the lag phase differs depending on the nature of the chemical substances. Bronopol, a substance frequently used in mobile toilets, prolongs the lag phase very significantly. On the other hand, Limonen or Citric Acid shorten the time of bacterial adaptation. The retention time of wastewater in the activation tank lies usually in the range of 6 to 8 hours. Regarding the extended lag phase the bacterial metabolism is insufficient for the properly treated wastewater.
ER  -

VÍTĚZOVÁ, Monika; Markéta KOPECKÁ; Tomáš VÍTĚZ a Tereza DOKULILOVÁ. Mobile toilets chemicals: its influence on activated sludge microorganisms. In \textit{4th International Conference on Pollution Control and Sustainable Environment}. 2018.

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