Growth and toxin production of Bacillus cereus strains in
reconstituted initial infant milk formula

J 2018

Growth and toxin production of Bacillus cereus strains in reconstituted initial infant milk formula

BURSOVA, S., L. NECIDOVA a Danka HARUŠTIAKOVÁ

Základní údaje

Originální název

Growth and toxin production of Bacillus cereus strains in reconstituted initial infant milk formula

Autoři

BURSOVA, S. (203 Česká republika), L. NECIDOVA (203 Česká republika) a Danka HARUŠTIAKOVÁ (703 Slovensko, garant, domácí)

Vydání

Food Control, Oxford, Elsevier Science Ltd, 2018, 0956-7135

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

21101 Food and beverages

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.248

Kód RIV

RIV/00216224:14310/18:00103467

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.foodcont.2017.05.006

UT WoS

000440390200041

Klíčová slova anglicky

Baranyi-Roberts model; Enterotoxins; Cereulide; lmmunochromatography

Štítky

14119612, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 4. 2024 12:24, Mgr. Michal Petr

Anotace

V originále

Bacillus cereus may cause two different types of foodborne illness: diarrheal and emetic syndromes. Dried infant formulae are at risk of contamination by the B. cereus, which is reflected by the requirement for the microbiological examination of foods listed in Regulation (EC) 2073/2005, as amended. This study evaluates the growth and toxin production potential of B. cereus in reconstituted dried infant milk formula. Powdered milk was inoculated with approximately 1 log and 3 log cfu g(-1) of spore suspension of toxigenic B. cereus strains and then stored as reconstituted milk for 48 h at 8 degrees C, 15 degrees C, and 24 degrees C. The growth of B. cereus, tested by the plate method, and production of the emetic toxin and enterotoxins, tested by the gold-labelled immunosorbent assay, were regularly detected during 48 h of storage. The Baranyi-Roberts model was used to study the growth potential of B. cereus strains. In reconstituted infant milk, B. cereus did not grow at 8 degrees C during 48 h of storage. B. cereus multiplied and formed toxins only at 15 degrees C and 24 degrees C. The growth curve of the bacteria stored at 15 degrees C included the lag and exponential phases, and the growth was not completed during the period. The growth of B. cereus at 24 degrees C was completed, peaking within 27 and 20 h, with the low and high inoculum sizes, respectively. Detectable amounts of toxins were formed during the bacterial growth of around 5 log cfu ml(-1) (non-hemolytic enterotoxin) and 7 log cfu ml(-1) (hemolysin BL and emetic toxin). Model experiments evaluated consumer risk from B. cereus toxins resulting from extended storage of reconstituted infant milk formula at improper temperatures. (C) 2017 Elsevier Ltd. All rights reserved.

Citovat

BURSOVA, S., L. NECIDOVA a Danka HARUŠTIAKOVÁ. Growth and toxin production of Bacillus cereus strains in reconstituted initial infant milk formula. Food Control. Oxford: Elsevier Science Ltd, 2018, roč. 93, NOV 2018, s. 334-343. ISSN 0956-7135. Dostupné z: https://dx.doi.org/10.1016/j.foodcont.2017.05.006.

@article{1428984,
   author = {Bursova, S. and Necidova, L. and Haruštiaková, Danka},
   article_location = {Oxford},
   article_number = {NOV 2018},
   doi = {http://dx.doi.org/10.1016/j.foodcont.2017.05.006},
   keywords = {Baranyi-Roberts model; Enterotoxins; Cereulide; lmmunochromatography},
   language = {eng},
   issn = {0956-7135},
   journal = {Food Control},
   title = {Growth and toxin production of Bacillus cereus strains in reconstituted initial infant milk formula},
   url = {http://dx.doi.org/10.1016/j.foodcont.2017.05.006},
   volume = {93},
   year = {2018}
}

TY  - JOUR
ID  - 1428984
AU  - Bursova, S. - Necidova, L. - Haruštiaková, Danka
PY  - 2018
TI  - Growth and toxin production of Bacillus cereus strains in reconstituted initial infant milk formula
JF  - Food Control
VL  - 93
IS  - NOV 2018
SP  - 334-343
EP  - 334-343
PB  - Elsevier Science Ltd
SN  - 09567135
KW  - Baranyi-Roberts model
KW  - Enterotoxins
KW  - Cereulide
KW  - lmmunochromatography
UR  - http://dx.doi.org/10.1016/j.foodcont.2017.05.006
N2  - Bacillus cereus may cause two different types of foodborne illness: diarrheal and emetic syndromes. Dried infant formulae are at risk of contamination by the B. cereus, which is reflected by the requirement for the microbiological examination of foods listed in Regulation (EC) 2073/2005, as amended. This study evaluates the growth and toxin production potential of B. cereus in reconstituted dried infant milk formula. Powdered milk was inoculated with approximately 1 log and 3 log cfu g(-1) of spore suspension of toxigenic B. cereus strains and then stored as reconstituted milk for 48 h at 8 degrees C, 15 degrees C, and 24 degrees C. The growth of B. cereus, tested by the plate method, and production of the emetic toxin and enterotoxins, tested by the gold-labelled immunosorbent assay, were regularly detected during 48 h of storage. The Baranyi-Roberts model was used to study the growth potential of B. cereus strains. In reconstituted infant milk, B. cereus did not grow at 8 degrees C during 48 h of storage. B. cereus multiplied and formed toxins only at 15 degrees C and 24 degrees C. The growth curve of the bacteria stored at 15 degrees C included the lag and exponential phases, and the growth was not completed during the period. The growth of B. cereus at 24 degrees C was completed, peaking within 27 and 20 h, with the low and high inoculum sizes, respectively. Detectable amounts of toxins were formed during the bacterial growth of around 5 log cfu ml(-1) (non-hemolytic enterotoxin) and 7 log cfu ml(-1) (hemolysin BL and emetic toxin). Model experiments evaluated consumer risk from B. cereus toxins resulting from extended storage of reconstituted infant milk formula at improper temperatures. (C) 2017 Elsevier Ltd. All rights reserved.
ER  -

BURSOVA, S., L. NECIDOVA a Danka HARUŠTIAKOVÁ. Growth and toxin production of Bacillus cereus strains in reconstituted initial infant milk formula. \textit{Food Control}. Oxford: Elsevier Science Ltd, 2018, roč.~93, NOV 2018, s.~334-343. ISSN~0956-7135. Dostupné z: https://dx.doi.org/10.1016/j.foodcont.2017.05.006.

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