MOLECULAR ANALYSIS OF PLASMIDS ISOLATED FROM ST398 STRAINS OF
STAPHYLOCOCCUS AUREUS

a 2018

MOLECULAR ANALYSIS OF PLASMIDS ISOLATED FROM ST398 STRAINS OF STAPHYLOCOCCUS AUREUS

RŮŽIČKOVÁ, Vladislava; Tibor BOTKA; Michaela KAVKOVÁ; Renata KARPÍŠKOVÁ; Ivana KOLÁČKOVÁ et al.

Základní údaje

Originální název

MOLECULAR ANALYSIS OF PLASMIDS ISOLATED FROM ST398 STRAINS OF STAPHYLOCOCCUS AUREUS

Autoři

RŮŽIČKOVÁ, Vladislava; Tibor BOTKA; Michaela KAVKOVÁ ; Renata KARPÍŠKOVÁ; Ivana KOLÁČKOVÁ a Jiří DOŠKAŘ

Vydání

International Symposium on Staphylococci and Staphylococcal Infections | ISSSI 2018: 23 - 26 August 2018, Copenhagen, Denmark, 2018

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10606 Microbiology

Stát vydavatele

Dánsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/18:00100669

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Staphylococcus aureus; plasmid; livestock associated MRSA

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 3. 2019 14:50, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

V originále

Background: The strains of S. aureus ST398 are associated worldwide with livestock, humans and food products. They contain a broad spectrum of plasmid-borne genes conferring antibiotic resistance having impact in pathogenicity of the veterinary strains. Objectives: The strains isolated from raw cow’s milk samples were genotypically analyzed, and their plasmids were characterized in detail. Methods: The MLST, spa typing, prophage and virulence genes content were carried out. The whole plasmid sequences were determined in two selected strains. Results: All the host strains of ST398 were resistant to oxacillin and other 2 – 8 antibiotics. The spa type t011 predominated over type t034. All the strains, with excepting of one isolate, harbored the prophage of serogroup A. Plasmids isolated from eight strains were assigned to six REAP types. Two plasmids were sequenced. They showed a different size, REAP fingerprint and a sequence structure. Amplification of the aad gene encoding aminoglycoside 6-adenylyltransferase is conserved in predominated group of small plasmids. One plasmid carrying the resistance gene to lincosamides was unique in the studied strains. It harbored a great region of identical sequences with plasmid of Staphylococcus chromogenes, which indicates on the interspecies horizontal transfer of plasmid genes. Conclusions: This study reports a research data of not yet well described plasmids of clonal ST398 strains of S. aureus having an impact in veterinary medicine and food industry. This work was supported by the grant from Ministry of Agriculture of the Czech Republic (QJ1510216) and from the MUNI/A/0824/2017.

Návaznosti

MUNI/A/0824/2017, interní kód MU

Název: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 6 (Akronym: MolBiolGen)

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů molekulární biologie a genetiky 6, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

QJ1510216, projekt VaV

Název: Fágová terapie infekcí vyvolaných Staphylococcus aureus v chovech hospodářských zvířat (Akronym: StafyFag)

Investor: Ministerstvo zemědělství ČR, Fágová terapie infekcí vyvolaných Staphylococcus aureus v chovech hospodářských zvířat

Citovat

RŮŽIČKOVÁ, Vladislava; Tibor BOTKA; Michaela KAVKOVÁ; Renata KARPÍŠKOVÁ; Ivana KOLÁČKOVÁ a Jiří DOŠKAŘ. MOLECULAR ANALYSIS OF PLASMIDS ISOLATED FROM ST398 STRAINS OF STAPHYLOCOCCUS AUREUS. In International Symposium on Staphylococci and Staphylococcal Infections | ISSSI 2018: 23 - 26 August 2018, Copenhagen, Denmark. 2018.

@proceedings{1430259,
   author = {Růžičková, Vladislava and Botka, Tibor and Kavková, Michaela and Karpíšková, Renata and Koláčková, Ivana and Doškař, Jiří},
   booktitle = {International Symposium on Staphylococci and Staphylococcal Infections | ISSSI 2018: 23 - 26 August 2018, Copenhagen, Denmark},
   keywords = {Staphylococcus aureus; plasmid; livestock associated MRSA},
   language = {eng},
   title = {MOLECULAR ANALYSIS OF PLASMIDS ISOLATED FROM ST398 STRAINS OF STAPHYLOCOCCUS AUREUS},
   url = {http://isssi2018.org},
   year = {2018}
}

TY  - CONF
ID  - 1430259
AU  - Růžičková, Vladislava - Botka, Tibor - Kavková, Michaela - Karpíšková, Renata - Koláčková, Ivana - Doškař, Jiří
PY  - 2018
TI  - MOLECULAR ANALYSIS OF PLASMIDS ISOLATED FROM ST398 STRAINS OF STAPHYLOCOCCUS AUREUS
KW  - Staphylococcus aureus
KW  - plasmid
KW  - livestock associated MRSA
UR  - http://isssi2018.org
N2  - Background: The strains of S. aureus ST398 are associated worldwide with livestock, humans and food products. They contain a broad spectrum of plasmid-borne genes conferring antibiotic resistance having impact in pathogenicity of the veterinary strains. Objectives: The strains isolated from raw cow’s milk samples were genotypically analyzed, and their plasmids were characterized in detail. Methods: The MLST, spa typing, prophage and virulence genes content were carried out. The whole plasmid sequences were determined in two selected strains. Results: All the host strains of ST398 were resistant to oxacillin and other 2 – 8 antibiotics. The spa type t011 predominated over type t034. All the strains, with excepting of one isolate, harbored the prophage of serogroup A. Plasmids isolated from eight strains were assigned to six REAP types. Two plasmids were sequenced. They showed a different size, REAP fingerprint and a sequence structure. Amplification of the aad gene encoding aminoglycoside 6-adenylyltransferase is conserved in predominated group of small plasmids. One plasmid carrying the resistance gene to lincosamides was unique in the studied strains. It harbored a great region of identical sequences with plasmid of Staphylococcus chromogenes, which indicates on the interspecies horizontal transfer of plasmid genes. Conclusions: This study reports a research data of not yet well described plasmids of clonal ST398 strains of S. aureus having an impact in veterinary medicine and food industry. This work was supported by the grant from Ministry of Agriculture of the Czech Republic (QJ1510216) and from the MUNI/A/0824/2017.
ER  -

RŮŽIČKOVÁ, Vladislava; Tibor BOTKA; Michaela KAVKOVÁ; Renata KARPÍŠKOVÁ; Ivana KOLÁČKOVÁ a Jiří DOŠKAŘ. MOLECULAR ANALYSIS OF PLASMIDS ISOLATED FROM ST398 STRAINS OF STAPHYLOCOCCUS AUREUS. In \textit{International Symposium on Staphylococci and Staphylococcal Infections $|$ ISSSI 2018: 23 - 26 August 2018, Copenhagen, Denmark}. 2018.

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