Development and application of MOL-PCR for the detection of
bacterial foodborne infections.

a 2018

Development and application of MOL-PCR for the detection of bacterial foodborne infections.

HUVAROVÁ, Veronika; Nikol RESLOVÁ a Petr KRÁLÍK

Základní údaje

Originální název

Development and application of MOL-PCR for the detection of bacterial foodborne infections.

Autoři

HUVAROVÁ, Veronika; Nikol RESLOVÁ a Petr KRÁLÍK

Vydání

XXVII. Tomáškovy dny mladých mikrobiologů, 2018

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/18:00103671

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-210-8963-1

Klíčová slova anglicky

foodborne pathogens; multiplex detection; MOL-PCR; MAGPIX; xMAP technology; Luminex

Příznaky

Recenzováno

Změněno: 14. 9. 2018 10:03, Mgr. Nikol Reslová, Ph.D.

Anotace

V originále

Multiplex oligonucleotide ligation PCR assay (MOL-PCR) is one method that can be used to detect microbial pathogens. It allows analysis of multiple types of markers, such as unique sequences, insertions, deletions, or single nucleotide polymorphisms (SNPs) in one multiplex reaction. For detecting a specific target sequence, a pair of MOLigo probes is proposed. Each pair of MOLigo probes is specific to a particular target sequence, but all the pairs contain the same sequence for placement of universal primers. MOLigo probes are connected to the target sequence and ligated during a separate ligation step to form a complex ssDNA that serves as a template for subsequent amplification with universal pair of primers (one fluorescently labeled) during PCR. One of the MOLigo probes also contains so-called TAG,it is a unique sequence by which the PCR products hybridize to a bead with a covalently coupled anti-TAG sequence. Each set of beads therefore carries a different anti-TAG sequence complementary to the TAG sequence in the MOLigo probe, while each set of beads has a distinct spectral address given by the combination of red and infrared fluorophores by which the polystyrene beads are filled. The whole reaction and subsequent analysis of the individual sets of beads with the PCR products is carried out in a MAGPix instrument connected to a computer. MOL-PCR has been optimized for the detection of bacterial pathogens caused alimentary infections as Listeria monocytogenes, Escherichia coli, Yersinia enterocolitica, Salmonella enterica subs. enterica and Campylobacter jejuni. The versatility of MOL-PCR was used in their simultaneous detection.

Citovat

HUVAROVÁ, Veronika; Nikol RESLOVÁ a Petr KRÁLÍK. Development and application of MOL-PCR for the detection of bacterial foodborne infections. In XXVII. Tomáškovy dny mladých mikrobiologů. 2018. ISBN 978-80-210-8963-1.

@proceedings{1435776,
   author = {Huvarová, Veronika and Reslová, Nikol and Králík, Petr},
   booktitle = {XXVII. Tomáškovy dny mladých mikrobiologů},
   keywords = {foodborne pathogens; multiplex detection; MOL-PCR; MAGPIX; xMAP technology; Luminex},
   language = {eng},
   isbn = {978-80-210-8963-1},
   title = {Development and application of MOL-PCR for the detection of bacterial foodborne infections.},
   year = {2018}
}

TY  - CONF
ID  - 1435776
AU  - Huvarová, Veronika - Reslová, Nikol - Králík, Petr
PY  - 2018
TI  - Development and application of MOL-PCR for the detection of bacterial foodborne infections.
SN  - 9788021089631
KW  - foodborne pathogens
KW  - multiplex detection
KW  - MOL-PCR
KW  - MAGPIX
KW  - xMAP technology
KW  - Luminex
N2  - Multiplex oligonucleotide ligation PCR assay (MOL-PCR) is one method that can be used to detect microbial pathogens. It allows analysis of multiple types of markers, such as unique sequences, insertions, deletions, or single nucleotide polymorphisms (SNPs) in one multiplex reaction. For detecting a specific target sequence, a pair of MOLigo probes is proposed. Each pair of MOLigo probes is specific to a particular target sequence, but all the pairs contain the same sequence for placement of universal primers. MOLigo probes are connected to the target sequence and ligated during a separate ligation step to form a complex ssDNA that serves as a template for subsequent amplification with universal pair of primers (one fluorescently labeled) during PCR. One of the MOLigo probes also contains so-called TAG,it is a unique sequence by which the PCR products hybridize to a bead with a covalently coupled anti-TAG sequence. Each set of beads therefore carries a different anti-TAG sequence complementary to the TAG sequence in the MOLigo probe, while each set of beads has a distinct spectral address given by the combination of red and infrared fluorophores by which the polystyrene beads are filled. The whole reaction and subsequent analysis of the individual sets of beads with the PCR products is carried out in a MAGPix instrument connected to a computer. MOL-PCR has been optimized for the detection of bacterial pathogens caused alimentary infections as Listeria monocytogenes, Escherichia coli, Yersinia enterocolitica, Salmonella enterica subs. enterica and Campylobacter jejuni. The versatility of MOL-PCR was used in their simultaneous detection.
ER  -

HUVAROVÁ, Veronika; Nikol RESLOVÁ a Petr KRÁLÍK. Development and application of MOL-PCR for the detection of bacterial foodborne infections. In \textit{XXVII. Tomáškovy dny mladých mikrobiologů}. 2018. ISBN~978-80-210-8963-1.

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