Study of retention behavior of anions by ion chromatography

D 2018

Study of retention behavior of anions by ion chromatography

ZIMA, Lukáš; Marta FARKOVÁ; Zdeněk FARKA a Přemysl LUBAL

Základní údaje

Originální název

Study of retention behavior of anions by ion chromatography

Autoři

ZIMA, Lukáš; Marta FARKOVÁ; Zdeněk FARKA a Přemysl LUBAL

Vydání

Brno, XVIII. Workshop of Biophysical Chemists and Electrochemists, od s. 72-73, 2 s. 2018

Nakladatel

Masarykova univerzita

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Odkazy

URL

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-210-9029-3

Klíčová slova anglicky

Separation; Ion chromatography; Determination of anions; Analysis of drinking water

Změněno: 15. 9. 2018 20:15, doc. Mgr. Zdeněk Farka, Ph.D.

Anotace

V originále

Ion chromatography (IC) belongs to the large group of separation methods that are based on separation of components in sample between two immiscible phases: mobile and stationary. In IC, the substances are separated due to different strengths of coulombic interactions with the stationary phase. Short analysis times, high separation efficiency, high reproducibility and low analysis costs make this technique useful in industry, clinical laboratories, food control and environmental analysis. This work is focused on the study of retention behavior of anions by IC and influence of experimental conditions (temperature, pH) on the separation efficiency. Mixtures of anions (F−, Cl−, NO2−, Br−, NO3−, PO43−, SO42− and I−) were analyzed on the Metrohm861 Advanced Compact IC ion chromatograph (Metrohm, Switzerland) with conductometric detector and Metrosep A Supp 4 - 250/4.0 annex column. The tested concentrations were in range of 0.08−10 mg/L. The analysis was performed with flow rate F of 1 ml/min, all samples were measured three times. The study of influence of temperature and pH on the separation was the main aim of this work. It was found that higher temperature and pH significantly decrease values of retention factors kA. The biggest difference was observed in case of iodide anions, where the retention time shifted from 22 to 14 min when the temperature was increased from 22 to 50 °C. The optimal analysis conditions were pH = 10.22 and t = 22 °C. The calibration provided LOD values between 3 μg/L (in case of Cl−) and 200 μg/L (in case of I−). To demonstrate the practical relevance of the method, real samples of drinking waters were analyzed. The achieved results were in good agreement with the concentration values declared by the producers. Ion chromatography represents a valuable approach for determination of anion concentrations. We have optimized the method with respect to effect of temperature and pH on the separation efficiency, the method is suitable for analysis of real samples. Due to discovered dependences it is possible to predict theoretical values of retention factors, which could be used to estimation of anion retention.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

MUNI/A/1237/2016, interní kód MU

Název: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2017

Investor: Masarykova univerzita, Vývoj metod a instrumentace pro analýzu biologicky významných látek 2017, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

ZIMA, Lukáš; Marta FARKOVÁ; Zdeněk FARKA a Přemysl LUBAL. Study of retention behavior of anions by ion chromatography. Online. In Libuše Trnková. XVIII. Workshop of Biophysical Chemists and Electrochemists. Brno: Masarykova univerzita, 2018, s. 72-73. ISBN 978-80-210-9029-3.

@inproceedings{1436498,
   author = {Zima, Lukáš and Farková, Marta and Farka, Zdeněk and Lubal, Přemysl},
   address = {Brno},
   booktitle = {XVIII. Workshop of Biophysical Chemists and Electrochemists},
   editor = {Libuše Trnková},
   keywords = {Separation; Ion chromatography; Determination of anions; Analysis of drinking water},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Brno},
   isbn = {978-80-210-9029-3},
   pages = {72-73},
   publisher = {Masarykova univerzita},
   title = {Study of retention behavior of anions by ion chromatography},
   url = {http://www.sci.muni.cz/~labifel/files/soubory/sbornik_2018.pdf#page=72},
   year = {2018}
}

TY  - CONF
ID  - 1436498
AU  - Zima, Lukáš - Farková, Marta - Farka, Zdeněk - Lubal, Přemysl
PY  - 2018
TI  - Study of retention behavior of anions by ion chromatography
PB  - Masarykova univerzita
CY  - Brno
SN  - 9788021090293
KW  - Separation
KW  - Ion chromatography
KW  - Determination of anions
KW  - Analysis of drinking water
UR  - http://www.sci.muni.cz/~labifel/files/soubory/sbornik_2018.pdf#page=72
N2  - Ion chromatography (IC) belongs to the large group of separation methods that are based on separation of components in sample between two immiscible phases: mobile and stationary. In IC, the substances are separated due to different strengths of coulombic interactions with the stationary phase. Short analysis times, high separation efficiency, high reproducibility and low analysis costs make this technique useful in industry, clinical laboratories, food control and environmental analysis. This work is focused on the study of retention behavior of anions by IC and influence of experimental conditions (temperature, pH) on the separation efficiency. Mixtures of anions (F−, Cl−, NO2−, Br−, NO3−, PO43−, SO42− and I−) were analyzed on the Metrohm861 Advanced Compact IC ion chromatograph (Metrohm, Switzerland) with conductometric detector and Metrosep A Supp 4 - 250/4.0 annex column. The tested concentrations were in range of 0.08−10 mg/L. The analysis was performed with flow rate F of 1 ml/min, all samples were measured three times. The study of influence of temperature and pH on the separation was the main aim of this work. It was found that higher temperature and pH significantly decrease values of retention factors kA. The biggest difference was observed in case of iodide anions, where the retention time shifted from 22 to 14 min when the temperature was increased from 22 to 50 °C. The optimal analysis conditions were pH = 10.22 and t = 22 °C. The calibration provided LOD values between 3 μg/L (in case of Cl−) and 200 μg/L (in case of I−). To demonstrate the practical relevance of the method, real samples of drinking waters were analyzed. The achieved results were in good agreement with the concentration values declared by the producers. Ion chromatography represents a valuable approach for determination of anion concentrations. We have optimized the method with respect to effect of temperature and pH on the separation efficiency, the method is suitable for analysis of real samples. Due to discovered dependences it is possible to predict theoretical values of retention factors, which could be used to estimation of anion retention.
ER  -

ZIMA, Lukáš; Marta FARKOVÁ; Zdeněk FARKA a Přemysl LUBAL. Study of retention behavior of anions by ion chromatography. Online. In Libuše Trnková. \textit{XVIII. Workshop of Biophysical Chemists and Electrochemists}. Brno: Masarykova univerzita, 2018, s.~72-73. ISBN~978-80-210-9029-3.

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