Effect of prostate cancer cell line supernatant on functional
polarization in macrophages

J 2018

Effect of prostate cancer cell line supernatant on functional polarization in macrophages

MAZALOVA, L.; Z. SLADEK; Martina RAUDENSKÁ; Jan BALVAN; Jaromír GUMULEC et al.

Základní údaje

Originální název

Effect of prostate cancer cell line supernatant on functional polarization in macrophages

Autoři

MAZALOVA, L.; Z. SLADEK; Martina RAUDENSKÁ ; Jan BALVAN; Jaromír GUMULEC a Michal MASAŘÍK

Vydání

Bratislava Medical Journal - Bratislavské lekárske listy, BRATISLAVA, Univerzita Komenského, 2018, 0006-9248

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.859

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/18:00104058

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

prostate; cancer; supernatant; macrophages; cytokines

Štítky

14110515, 14110518, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 5. 2019 14:10, Soňa Böhmová

Anotace

V originále

OBJECTIVES: We aimed on effect of supernatant derived from prostate cancer cell line PC-3 on M1/M2 functional polarization in macrophages. BACKGROUND: Cytokines play an important role in carcinogenesis. Most of them are produced by macrophages. Macrophages are divided into groups M1 or M2. Classical phenotype macrophages M1 support pro-inflammatory effects and produce pro-inflammatory cytokines, such as interleukin 6 (IL-6), interleukin 12 (IL-12), tumor necrosis factor alpha (TNF-alpha). Macrophages exhibiting a phenotype M2 secrete anti-inflammatory cytokines, e.g. interleukin 10 (IL-10), transforming growth factor beta (TGF-beta). METHODS: Peripheral blood monocytes were cultivated for 7 days and during this time went through a differentiation into macrophages. Macrophages were stimulated for 24 hours by lipopolysaccharide (LPS) as a positive control and cultivated with supernatant for another 24 hours. RESULTS: Macrophages cultivated without LPS and without supernatant were used as negative control. Relative expression of IL-6, IL-10, IL-12 and TNF-alpha was measured by Quantitative real-time PCR. Expression of pro-inflammatory cytokines was lower in macrophages with supernatant compared to positive control. CONCLUSION: Expression of pro-inflammatory cytokines was lower in macrophages with supernatant (M phi+sup) compared to positive control (M phi+LPS). Effect of the supernatant on expression of IL-6, IL-10, IL-12 and TNF-alpha was not confirmed (Tab. 1, Fig. 5, Ref. 15). Text in PDF www.elis.sk.

Citovat

MAZALOVA, L.; Z. SLADEK; Martina RAUDENSKÁ; Jan BALVAN; Jaromír GUMULEC a Michal MASAŘÍK. Effect of prostate cancer cell line supernatant on functional polarization in macrophages. Bratislava Medical Journal - Bratislavské lekárske listy. BRATISLAVA: Univerzita Komenského, 2018, roč. 119, č. 8, s. 516-521. ISSN 0006-9248. Dostupné z: https://doi.org/10.4149/BLL_2018_095.

@article{1450136,
   author = {Mazalova, L. and Sladek, Z. and Raudenská, Martina and Balvan, Jan and Gumulec, Jaromír and Masařík, Michal},
   article_location = {BRATISLAVA},
   article_number = {8},
   doi = {https://doi.org/10.4149/BLL_2018_095},
   keywords = {prostate; cancer; supernatant; macrophages; cytokines},
   language = {eng},
   issn = {0006-9248},
   journal = {Bratislava Medical Journal - Bratislavské lekárske listy},
   title = {Effect of prostate cancer cell line supernatant on functional polarization in macrophages},
   volume = {119},
   year = {2018}
}

TY  - JOUR
ID  - 1450136
AU  - Mazalova, L. - Sladek, Z. - Raudenská, Martina - Balvan, Jan - Gumulec, Jaromír - Masařík, Michal
PY  - 2018
TI  - Effect of prostate cancer cell line supernatant on functional polarization in macrophages
JF  - Bratislava Medical Journal - Bratislavské lekárske listy
VL  - 119
IS  - 8
SP  - 516-521
EP  - 516-521
PB  - Univerzita Komenského
SN  - 00069248
KW  - prostate
KW  - cancer
KW  - supernatant
KW  - macrophages
KW  - cytokines
N2  - OBJECTIVES: We aimed on effect of supernatant derived from prostate cancer cell line PC-3 on M1/M2 functional polarization in macrophages. BACKGROUND: Cytokines play an important role in carcinogenesis. Most of them are produced by macrophages. Macrophages are divided into groups M1 or M2. Classical phenotype macrophages M1 support pro-inflammatory effects and produce pro-inflammatory cytokines, such as interleukin 6 (IL-6), interleukin 12 (IL-12), tumor necrosis factor alpha (TNF-alpha). Macrophages exhibiting a phenotype M2 secrete anti-inflammatory cytokines, e.g. interleukin 10 (IL-10), transforming growth factor beta (TGF-beta). METHODS: Peripheral blood monocytes were cultivated for 7 days and during this time went through a differentiation into macrophages. Macrophages were stimulated for 24 hours by lipopolysaccharide (LPS) as a positive control and cultivated with supernatant for another 24 hours. RESULTS: Macrophages cultivated without LPS and without supernatant were used as negative control. Relative expression of IL-6, IL-10, IL-12 and TNF-alpha was measured by Quantitative real-time PCR. Expression of pro-inflammatory cytokines was lower in macrophages with supernatant compared to positive control. CONCLUSION: Expression of pro-inflammatory cytokines was lower in macrophages with supernatant (M phi+sup) compared to positive control (M phi+LPS). Effect of the supernatant on expression of IL-6, IL-10, IL-12 and TNF-alpha was not confirmed (Tab. 1, Fig. 5, Ref. 15). Text in PDF www.elis.sk.
ER  -

MAZALOVA, L.; Z. SLADEK; Martina RAUDENSKÁ; Jan BALVAN; Jaromír GUMULEC a Michal MASAŘÍK. Effect of prostate cancer cell line supernatant on functional polarization in macrophages. \textit{Bratislava Medical Journal - Bratislavské lekárske listy}. BRATISLAVA: Univerzita Komenského, 2018, roč.~119, č.~8, s.~516-521. ISSN~0006-9248. Dostupné z: https://doi.org/10.4149/BLL\_{}2018\_{}095.

Přehled o publikaci