AgCu bimetallic nanoparticles modified by polyvinyl alcohol -
the cells viability study in vitro

J 2018

AgCu bimetallic nanoparticles modified by polyvinyl alcohol - the cells viability study in vitro

BERNARD, Vladan; Ondřej ZOBAČ; Marcela VLKOVÁ; Vojtěch MORNSTEIN; Jiří SOPOUŠEK et al.

Základní údaje

Originální název

AgCu bimetallic nanoparticles modified by polyvinyl alcohol - the cells viability study in vitro

Autoři

BERNARD, Vladan ; Ondřej ZOBAČ; Marcela VLKOVÁ; Vojtěch MORNSTEIN a Jiří SOPOUŠEK

Vydání

Lékař a technika, Praha, Fakulta biomedicínského inženýrství ČVUT, 2018, 0301-5491

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

21001 Nano-materials

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/18:00104068

Organizační jednotka

Lékařská fakulta

EID Scopus

2-s2.0-85049775759

Klíčová slova anglicky

AgCu NPs; Cells A2780; nanoparticle; viability

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 5. 2019 14:13, Soňa Böhmová

Anotace

V originále

The effects of elementary metallic nanoparticles on living objects as cytotoxicity or antibacterial activity are widely known. Ag nanoparticles are a suitable and well known example. Nanoparticles formed by an alloy of Ag and Cu stabilized by polyvinyl alcohol were examined on two human tumor cells - ovarian carcinoma cells A2780 and skin melanoma cells A375. Bimetalic AgCu nanoparticles were synthetized by using a method of chemical co-reduction of silver nitrate and copper (II) nitrate hydrate. The nanoparticles were characterized by electron microscopy and by measurement of zeta potential. Cell viability was tested by using an MTT (tetrazole colorimetric viability assay) test. The effect on cell apoptosis and necrosis was measured by using flow cytometry. The experimental results indicate a differentiated impact of nanoparticles on the cells used. A more significant effect of viability decrease was observed for A2780 cells. The cell death caused by the nanoparticles used was observed particularly in the form of initial and advanced apoptosis for both cells lines, necrosis was observed to a lesser extent. The synthetized bimetallic nanoparticles seem to be a suitable candidate for targeted suppression of cell proliferation.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

LD11046, projekt VaV

Název: Fázové diagramy nanoslitin, jejich výpočty a ověření. (Akronym: PHADINANOAL)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Fázové diagramy nanoslitin, jejich výpočty a ověření.

Citovat

BERNARD, Vladan; Ondřej ZOBAČ; Marcela VLKOVÁ; Vojtěch MORNSTEIN a Jiří SOPOUŠEK. AgCu bimetallic nanoparticles modified by polyvinyl alcohol - the cells viability study in vitro. Lékař a technika. Praha: Fakulta biomedicínského inženýrství ČVUT, 2018, roč. 48, č. 1, s. 5-10. ISSN 0301-5491.

@article{1450556,
   author = {Bernard, Vladan and Zobač, Ondřej and Vlková, Marcela and Mornstein, Vojtěch and Sopoušek, Jiří},
   article_location = {Praha},
   article_number = {1},
   keywords = {AgCu NPs; Cells A2780; nanoparticle; viability},
   language = {eng},
   issn = {0301-5491},
   journal = {Lékař a technika},
   title = {AgCu bimetallic nanoparticles modified by polyvinyl alcohol - the cells viability study in vitro},
   volume = {48},
   year = {2018}
}

TY  - JOUR
ID  - 1450556
AU  - Bernard, Vladan - Zobač, Ondřej - Vlková, Marcela - Mornstein, Vojtěch - Sopoušek, Jiří
PY  - 2018
TI  - AgCu bimetallic nanoparticles modified by polyvinyl alcohol - the cells viability study in vitro
JF  - Lékař a technika
VL  - 48
IS  - 1
SP  - 5-10
EP  - 5-10
PB  - Fakulta biomedicínského inženýrství ČVUT
SN  - 03015491
KW  - AgCu NPs
KW  - Cells A2780
KW  - nanoparticle
KW  - viability
N2  - The effects of elementary metallic nanoparticles on living objects as cytotoxicity or antibacterial activity are widely known. Ag nanoparticles are a suitable and well known example. Nanoparticles formed by an alloy of Ag and Cu stabilized by polyvinyl alcohol were examined on two human tumor cells - ovarian carcinoma cells A2780 and skin melanoma cells A375. Bimetalic AgCu nanoparticles were synthetized by using a method of chemical co-reduction of silver nitrate and copper (II) nitrate hydrate. The nanoparticles were characterized by electron microscopy and by measurement of zeta potential. Cell viability was tested by using an MTT (tetrazole colorimetric viability assay) test. The effect on cell apoptosis and necrosis was measured by using flow cytometry. The experimental results indicate a differentiated impact of nanoparticles on the cells used. A more significant effect of viability decrease was observed for A2780 cells. The cell death caused by the nanoparticles used was observed particularly in the form of initial and advanced apoptosis for both cells lines, necrosis was observed to a lesser extent. The synthetized bimetallic nanoparticles seem to be a suitable candidate for targeted suppression of cell proliferation.
ER  -

BERNARD, Vladan; Ondřej ZOBAČ; Marcela VLKOVÁ; Vojtěch MORNSTEIN a Jiří SOPOUŠEK. AgCu bimetallic nanoparticles modified by polyvinyl alcohol - the cells viability study in vitro. \textit{Lékař a technika}. Praha: Fakulta biomedicínského inženýrství ČVUT, 2018, roč.~48, č.~1, s.~5-10. ISSN~0301-5491.

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