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@article{1453617,
author = {Breza, Jan and Soltysova, Andrea and Hudecova, Sona and Penesova, Adela and Szadvári, Ivan and Babula, Petr and Chovancova, Barbora and Lencesova, Lubomira and Pos, Ondrej and Breza, Jan and Ondrias, Karol and Križanová, Oľga},
article_location = {London},
article_number = {591},
doi = {http://dx.doi.org/10.1186/s12885-018-4508-1},
keywords = {Cystathionine-beta-synthase; Cystathionine gamma-lyase; 3-Mercaptopyruvate sulfurtransferase; Apoptosis; Clear cell renal cell carcinoma},
language = {eng},
issn = {1471-2407},
journal = {BMC Cancer},
title = {Endogenous H2S producing enzymes are involved in apoptosis induction in clear cell renal cell carcinoma},
url = {http://dx.doi.org/10.1186/s12885-018-4508-1},
volume = {18},
year = {2018}
}
TY - JOUR
ID - 1453617
AU - Breza, Jan - Soltysova, Andrea - Hudecova, Sona - Penesova, Adela - Szadvári, Ivan - Babula, Petr - Chovancova, Barbora - Lencesova, Lubomira - Pos, Ondrej - Breza, Jan - Ondrias, Karol - Križanová, Oľga
PY - 2018
TI - Endogenous H2S producing enzymes are involved in apoptosis induction in clear cell renal cell carcinoma
JF - BMC Cancer
VL - 18
IS - 591
SP - 1-8
EP - 1-8
PB - BioMed Central
SN - 14712407
KW - Cystathionine-beta-synthase
KW - Cystathionine gamma-lyase
KW - 3-Mercaptopyruvate sulfurtransferase
KW - Apoptosis
KW - Clear cell renal cell carcinoma
UR - http://dx.doi.org/10.1186/s12885-018-4508-1
N2 - Background: Knowledge about the expression and thus a role of enzymes that produce endogenous H2S-cystathionine-beta-synthase, cystathionine gamma-lyase and mercaptopyruvate sulfurtransferase - in renal tumors is still controversial. In this study we aimed to determine the expression of these enzymes relatively to the expression in unaffected part of kidney from the same patient and to found relation of these changes to apoptosis. To evaluate patient's samples, microarray and immunohistochemistry was used. Methods: To determine the physiological importance, we used RCC4 stable cell line derived from clear cell renal cell carcinoma, where apoptosis induction by a mixture of five chemotherapeutics with/without silencing of H2S-producing enzymes was detected. Immunofluorescence was used to determine each enzyme in the cells. Results: In clear cell renal cell carcinomas, expression of H2S-producing enzymes was mostly decreased compared to a part of kidney that was distal from the tumor. To evaluate a potential role of H2S-producing enzymes in the apoptosis induction, we used RCC4 stable cell line. We have found that silencing of cystathionine-beta-synthase and cystathionine.-lyase prevented induction of apoptosis. Immunofluorescence staining clearly showed that these enzymes were upregulated during apoptosis in RCC4 cells. Conclusion: Based on these results we concluded that in clear cell renal cell carcinoma, reduced expression of the H2S-producing enzymes, mainly cystathionine gamma-lyase, might contribute to a resistance to the induction of apoptosis. Increased production of the endogenous H2S, or donation from the external sources might be of a therapeutic importance in these tumors.
ER -
BREZA, Jan, Andrea SOLTYSOVA, Sona HUDECOVA, Adela PENESOVA, Ivan SZADVÁRI, Petr BABULA, Barbora CHOVANCOVA, Lubomira LENCESOVA, Ondrej POS, Jan BREZA, Karol ONDRIAS a Oľga KRIŽANOVÁ. Endogenous H2S producing enzymes are involved in apoptosis induction in clear cell renal cell carcinoma. \textit{BMC Cancer}. London: BioMed Central, 2018, roč.~18, č.~591, s.~1-8. ISSN~1471-2407. Dostupné z: https://dx.doi.org/10.1186/s12885-018-4508-1.
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