Inhibition of SHIP2 activity inhibits cell migration and could
prevent metastasis in breast cancer cells

J 2018

Inhibition of SHIP2 activity inhibits cell migration and could prevent metastasis in breast cancer cells

GHOSH, Somadri; Samuel SCOZZARO; Ana Raque RAMOS; Seprimebastien DELCAMBRE; Cleprimement CHEVALIER et al.

Základní údaje

Originální název

Inhibition of SHIP2 activity inhibits cell migration and could prevent metastasis in breast cancer cells

Autoři

GHOSH, Somadri; Samuel SCOZZARO; Ana Raque RAMOS; Seprimebastien DELCAMBRE; Cleprimement CHEVALIER; Pavel KREJČÍ a Christophe ERNEUX

Vydání

Journal of Cell Science, Cambridge, Company of Biologists Ltd. 2018, 0021-9533

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.517

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/18:00104188

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

SHIP2; Phosphoinositide; Cell migration; Breast cancer cell

Štítky

14110513, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 2. 2019 14:56, Soňa Böhmová

Anotace

V originále

Metastasis of breast cancer cells to distant organs is responsible for similar to 50% of breast cancer-related deaths in women worldwide. SHIP2 (also known as INPPL1) is a phosphoinositide 5-phosphatase for phosphatidylinositol (3,4,5)-trisphosphate [PI(3,4,5)P3] and phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2]. Here we show, through depletion of SHIP2 in triple negative MDA-MB-231 cells and the use of SHIP2 inhibitors, that cell migration appears to be positively controlled by SHIP2. The effect of SHIP2 on migration, as observed in MDA-MB-231 cells, appears to be mediated by PI(3,4) P2. Adhesion on fibronectin is always increased in SHIP2-depleted cells. Apoptosis measured in MDA-MB-231 cells is also increased in SHIP2-depleted cells as compared to control cells. In xenograft mice, SHIP2-depleted MDA-MB-231 cells form significantly smaller tumors than those formed by control cells and less metastasis is detected in lung sections. Our data reveal a general role for SHIP2 in the control of cell migration in breast cancer cells and a second messenger role for PI(3,4) P2 in the migration mechanism. In MDA-MB-231 cells, SHIP2 has a function in apoptosis in cells incubated in vitro and in mouse tumor-derived cells, which could account for its role on tumor growth determined in vivo.

Citovat

GHOSH, Somadri; Samuel SCOZZARO; Ana Raque RAMOS; Seprimebastien DELCAMBRE; Cleprimement CHEVALIER; Pavel KREJČÍ a Christophe ERNEUX. Inhibition of SHIP2 activity inhibits cell migration and could prevent metastasis in breast cancer cells. Journal of Cell Science. Cambridge: Company of Biologists Ltd., 2018, roč. 131, č. 16, s. 1-12. ISSN 0021-9533. Dostupné z: https://doi.org/10.1242/jcs.216408.

@article{1455136,
   author = {Ghosh, Somadri and Scozzaro, Samuel and Ramos, Ana Raque and Delcambre, Seprimebastien and Chevalier, Cleprimement and Krejčí, Pavel and Erneux, Christophe},
   article_location = {Cambridge},
   article_number = {16},
   doi = {https://doi.org/10.1242/jcs.216408},
   keywords = {SHIP2; Phosphoinositide; Cell migration; Breast cancer cell},
   language = {eng},
   issn = {0021-9533},
   journal = {Journal of Cell Science},
   title = {Inhibition of SHIP2 activity inhibits cell migration and could prevent metastasis in breast cancer cells},
   volume = {131},
   year = {2018}
}

TY  - JOUR
ID  - 1455136
AU  - Ghosh, Somadri - Scozzaro, Samuel - Ramos, Ana Raque - Delcambre, Seprimebastien - Chevalier, Cleprimement - Krejčí, Pavel - Erneux, Christophe
PY  - 2018
TI  - Inhibition of SHIP2 activity inhibits cell migration and could prevent metastasis in breast cancer cells
JF  - Journal of Cell Science
VL  - 131
IS  - 16
SP  - 1-12
EP  - 1-12
PB  - Company of Biologists Ltd.
SN  - 00219533
KW  - SHIP2
KW  - Phosphoinositide
KW  - Cell migration
KW  - Breast cancer cell
N2  - Metastasis of breast cancer cells to distant organs is responsible for similar to 50% of breast cancer-related deaths in women worldwide. SHIP2 (also known as INPPL1) is a phosphoinositide 5-phosphatase for phosphatidylinositol (3,4,5)-trisphosphate [PI(3,4,5)P3] and phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2]. Here we show, through depletion of SHIP2 in triple negative MDA-MB-231 cells and the use of SHIP2 inhibitors, that cell migration appears to be positively controlled by SHIP2. The effect of SHIP2 on migration, as observed in MDA-MB-231 cells, appears to be mediated by PI(3,4) P2. Adhesion on fibronectin is always increased in SHIP2-depleted cells. Apoptosis measured in MDA-MB-231 cells is also increased in SHIP2-depleted cells as compared to control cells. In xenograft mice, SHIP2-depleted MDA-MB-231 cells form significantly smaller tumors than those formed by control cells and less metastasis is detected in lung sections. Our data reveal a general role for SHIP2 in the control of cell migration in breast cancer cells and a second messenger role for PI(3,4) P2 in the migration mechanism. In MDA-MB-231 cells, SHIP2 has a function in apoptosis in cells incubated in vitro and in mouse tumor-derived cells, which could account for its role on tumor growth determined in vivo.
ER  -

GHOSH, Somadri; Samuel SCOZZARO; Ana Raque RAMOS; Seprimebastien DELCAMBRE; Cleprimement CHEVALIER; Pavel KREJČÍ a Christophe ERNEUX. Inhibition of SHIP2 activity inhibits cell migration and could prevent metastasis in breast cancer cells. \textit{Journal of Cell Science}. Cambridge: Company of Biologists Ltd., 2018, roč.~131, č.~16, s.~1-12. ISSN~0021-9533. Dostupné z: https://doi.org/10.1242/jcs.216408.

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