Inhibition of SHIP2 activity inhibits cell migration and could
prevent metastasis in breast cancer cells

J 2018

Inhibition of SHIP2 activity inhibits cell migration and could prevent metastasis in breast cancer cells

GHOSH, Somadri, Samuel SCOZZARO, Ana Raque RAMOS, Seprimebastien DELCAMBRE, Cleprimement CHEVALIER et. al.

Basic information

Original name

Inhibition of SHIP2 activity inhibits cell migration and could prevent metastasis in breast cancer cells

Authors

GHOSH, Somadri (56 Belgium), Samuel SCOZZARO (56 Belgium), Ana Raque RAMOS (56 Belgium), Seprimebastien DELCAMBRE (56 Belgium), Cleprimement CHEVALIER (56 Belgium), Pavel KREJČÍ (203 Czech Republic, belonging to the institution) and Christophe ERNEUX (56 Belgium, guarantor)

Edition

Journal of Cell Science, Cambridge, Company of Biologists Ltd. 2018, 0021-9533

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.517

RIV identification code

RIV/00216224:14110/18:00104188

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1242/jcs.216408

UT WoS

000443438900007

Keywords in English

SHIP2; Phosphoinositide; Cell migration; Breast cancer cell

Abstract

V originále

Metastasis of breast cancer cells to distant organs is responsible for similar to 50% of breast cancer-related deaths in women worldwide. SHIP2 (also known as INPPL1) is a phosphoinositide 5-phosphatase for phosphatidylinositol (3,4,5)-trisphosphate [PI(3,4,5)P3] and phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2]. Here we show, through depletion of SHIP2 in triple negative MDA-MB-231 cells and the use of SHIP2 inhibitors, that cell migration appears to be positively controlled by SHIP2. The effect of SHIP2 on migration, as observed in MDA-MB-231 cells, appears to be mediated by PI(3,4) P2. Adhesion on fibronectin is always increased in SHIP2-depleted cells. Apoptosis measured in MDA-MB-231 cells is also increased in SHIP2-depleted cells as compared to control cells. In xenograft mice, SHIP2-depleted MDA-MB-231 cells form significantly smaller tumors than those formed by control cells and less metastasis is detected in lung sections. Our data reveal a general role for SHIP2 in the control of cell migration in breast cancer cells and a second messenger role for PI(3,4) P2 in the migration mechanism. In MDA-MB-231 cells, SHIP2 has a function in apoptosis in cells incubated in vitro and in mouse tumor-derived cells, which could account for its role on tumor growth determined in vivo.

Citovat

GHOSH, Somadri, Samuel SCOZZARO, Ana Raque RAMOS, Seprimebastien DELCAMBRE, Cleprimement CHEVALIER, Pavel KREJČÍ and Christophe ERNEUX. Inhibition of SHIP2 activity inhibits cell migration and could prevent metastasis in breast cancer cells. Journal of Cell Science. Cambridge: Company of Biologists Ltd., 2018, vol. 131, No 16, p. 1-12. ISSN 0021-9533. Available from: https://dx.doi.org/10.1242/jcs.216408.

@article{1455136,
   author = {Ghosh, Somadri and Scozzaro, Samuel and Ramos, Ana Raque and Delcambre, Seprimebastien and Chevalier, Cleprimement and Krejčí, Pavel and Erneux, Christophe},
   article_location = {Cambridge},
   article_number = {16},
   doi = {http://dx.doi.org/10.1242/jcs.216408},
   keywords = {SHIP2; Phosphoinositide; Cell migration; Breast cancer cell},
   language = {eng},
   issn = {0021-9533},
   journal = {Journal of Cell Science},
   title = {Inhibition of SHIP2 activity inhibits cell migration and could prevent metastasis in breast cancer cells},
   volume = {131},
   year = {2018}
}

TY  - JOUR
ID  - 1455136
AU  - Ghosh, Somadri - Scozzaro, Samuel - Ramos, Ana Raque - Delcambre, Seprimebastien - Chevalier, Cleprimement - Krejčí, Pavel - Erneux, Christophe
PY  - 2018
TI  - Inhibition of SHIP2 activity inhibits cell migration and could prevent metastasis in breast cancer cells
JF  - Journal of Cell Science
VL  - 131
IS  - 16
SP  - 1-12
EP  - 1-12
PB  - Company of Biologists Ltd.
SN  - 00219533
KW  - SHIP2
KW  - Phosphoinositide
KW  - Cell migration
KW  - Breast cancer cell
N2  - Metastasis of breast cancer cells to distant organs is responsible for similar to 50% of breast cancer-related deaths in women worldwide. SHIP2 (also known as INPPL1) is a phosphoinositide 5-phosphatase for phosphatidylinositol (3,4,5)-trisphosphate [PI(3,4,5)P3] and phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2]. Here we show, through depletion of SHIP2 in triple negative MDA-MB-231 cells and the use of SHIP2 inhibitors, that cell migration appears to be positively controlled by SHIP2. The effect of SHIP2 on migration, as observed in MDA-MB-231 cells, appears to be mediated by PI(3,4) P2. Adhesion on fibronectin is always increased in SHIP2-depleted cells. Apoptosis measured in MDA-MB-231 cells is also increased in SHIP2-depleted cells as compared to control cells. In xenograft mice, SHIP2-depleted MDA-MB-231 cells form significantly smaller tumors than those formed by control cells and less metastasis is detected in lung sections. Our data reveal a general role for SHIP2 in the control of cell migration in breast cancer cells and a second messenger role for PI(3,4) P2 in the migration mechanism. In MDA-MB-231 cells, SHIP2 has a function in apoptosis in cells incubated in vitro and in mouse tumor-derived cells, which could account for its role on tumor growth determined in vivo.
ER  -

GHOSH, Somadri, Samuel SCOZZARO, Ana Raque RAMOS, Seprimebastien DELCAMBRE, Cleprimement CHEVALIER, Pavel KREJČÍ and Christophe ERNEUX. Inhibition of SHIP2 activity inhibits cell migration and could prevent metastasis in breast cancer cells. \textit{Journal of Cell Science}. Cambridge: Company of Biologists Ltd., 2018, vol.~131, No~16, p.~1-12. ISSN~0021-9533. Available from: https://dx.doi.org/10.1242/jcs.216408.

Detailed Information on Publication Record