2018
MALDI mass spectrometry imaging at acquisition rates above 100 pixel/s
PREISLER, JanZákladní údaje
Originální název
MALDI mass spectrometry imaging at acquisition rates above 100 pixel/s
Název anglicky
MALDI mass spectrometry imaging at acquisition rates above 100 pixel/s
Autoři
Vydání
Brno, Czech Republic, CECE 2018 15th International Interdisciplinary Meeting on Bioanalysis, od s. 19, 33, 2 s. 2018
Nakladatel
Institute of Analytical Chemistry of the CAS, v. v. i.
Další údaje
Jazyk
čeština
Typ výsledku
Stať ve sborníku
Obor
10406 Analytical chemistry
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Forma vydání
tištěná verze "print"
Odkazy
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14310/18:00101298
Organizační jednotka
Přírodovědecká fakulta
ISBN
978-80-904959-5-1
Klíčová slova anglicky
MALDI; MSI; mass spectromerty imaging; high throughput
Změněno: 29. 10. 2018 13:38, prof. Mgr. Jan Preisler, Ph.D.
V originále
The practicality of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) applied to molecular imaging of biological tissues is limited by the analysis speed. A relatively low-speed motion of xy stages is one of the factors reducing the rate with which fresh sample material can be supplied to the laser spot. The sample scan rate in our laboratory-built high-throughput imaging time-of-flight (TOF) mass spectrometer was significantly improved through the use of a galvanometer-based optical scanner performing fast laser beam repositioning on a target plate. [1] The laser beam was focused via a modified grid into a 10 um round spot. The modified optical system of our MALDI TOF mass spectrometer allowed the acquisition of high-resolution MS images with a well-defined pixel size at acquisition rates up to ~150 pixels/s. The influence of selected parameters on the total MS imaging time was examined. The new scanning technique was employed to display the distribution of an antitumor agent in 3D colorectal adenocarcinoma cell aggregates. A single MS image comprising 100 × 100 pixels with 10 um lateral resolution was recorded in approximately 70 seconds.
Anglicky
The practicality of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) applied to molecular imaging of biological tissues is limited by the analysis speed. A relatively low-speed motion of xy stages is one of the factors reducing the rate with which fresh sample material can be supplied to the laser spot. The sample scan rate in our laboratory-built high-throughput imaging time-of-flight (TOF) mass spectrometer was significantly improved through the use of a galvanometer-based optical scanner performing fast laser beam repositioning on a target plate. [1] The laser beam was focused via a modified grid into a 10 um round spot. The modified optical system of our MALDI TOF mass spectrometer allowed the acquisition of high-resolution MS images with a well-defined pixel size at acquisition rates up to ~150 pixels/s. The influence of selected parameters on the total MS imaging time was examined. The new scanning technique was employed to display the distribution of an antitumor agent in 3D colorectal adenocarcinoma cell aggregates. A single MS image comprising 100 × 100 pixels with 10 um lateral resolution was recorded in approximately 70 seconds.
Návaznosti
| GA15-05387S, projekt VaV |
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| MUNI/G/0974/2016, interní kód MU |
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