Colicin F-Y inhibits pathogenic Yersinia enterocolitica in mice

J 2018

Colicin F-Y inhibits pathogenic Yersinia enterocolitica in mice

BOSÁK, Juraj; Lenka MICENKOVÁ; Matěj HRALA; Katarína POMORSKÁ; Michaela BOSÁKOVÁ et. al.

Základní údaje

Originální název

Colicin F-Y inhibits pathogenic Yersinia enterocolitica in mice

Autoři

BOSÁK, Juraj; Lenka MICENKOVÁ; Matěj HRALA; Katarína POMORSKÁ; Michaela BOSÁKOVÁ; Pavel KREJČÍ; Eduard GÖPFERT; Martin FALDYNA a David ŠMAJS

Vydání

Scientific reports, London, NATURE PUBLISHING GROUP, 2018, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.011

Kód RIV

RIV/00216224:14110/18:00101322

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1038/s41598-018-30729-7

UT WoS

000441775900006

EID Scopus

2-s2.0-85051697166

Klíčová slova anglicky

Yersinia enterocolitica; Colicin F-Y

Štítky

14110513, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 3. 2019 10:26, Soňa Böhmová

Anotace

V originále

Yersiniosis belongs to the common foodborne diseases around the world, and frequently manifests as diarrhea that can be treated with probiotics. Colicin F-Y is an antibacterial agent produced by bacteria and it is capable of specific growth inhibition of Yersinia enterocolitica, the causative agent of gastrointestinal yersiniosis. In this study, recombinant E. coli producing colicin F-Y were constructed, using both known probiotic strains EcH22 and EcColinfant, and the newly isolated murine strains Ec1127 and Ec1145. All E. coli strains producing colicin FY inhibited growth of pathogenic Y. enterocolitica during co-cultivation in vitro. In dysbiotic mice treated with streptomycin, E. coli strains producing colicin F-Y inhibited progression of Y. enterocolitica infections. This growth inhibition was not observed in mice with normal gut microflora, likely due to insufficient colonization capacity of E. coli strains and/or due to spatial differences in intestinal niches. Isogenic Y. enterocolitica producing colicin F-Y was constructed and shown to inhibit pathogenic Y. enterocolitica in mice with normal microflora. Evidence of in vivo antimicrobial activity of colicin F-Y may have utility in the treatment of Y. enterocolitica infections.

Návaznosti

EF16_013/0001761, projekt VaV

Název: RECETOX RI

GA16-21649S, projekt VaV

Název: Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

Investor: Grantová agentura ČR, Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

ROZV/25/LF/2017, interní kód MU

Název: LF - Příspěvek na IP 2017

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, LF - Příspěvek na IP 2017, Interní rozvojové projekty

Citovat

BOSÁK, Juraj; Lenka MICENKOVÁ; Matěj HRALA; Katarína POMORSKÁ; Michaela BOSÁKOVÁ; Pavel KREJČÍ; Eduard GÖPFERT; Martin FALDYNA a David ŠMAJS. Colicin F-Y inhibits pathogenic Yersinia enterocolitica in mice. Scientific reports. London: NATURE PUBLISHING GROUP, 2018, roč. 8, č. 12242, s. 1-12. ISSN 2045-2322. Dostupné z: https://doi.org/10.1038/s41598-018-30729-7.

@article{1461576,
   author = {Bosák, Juraj and Micenková, Lenka and Hrala, Matěj and Pomorská, Katarína and Bosáková, Michaela and Krejčí, Pavel and Göpfert, Eduard and Faldyna, Martin and Šmajs, David},
   article_location = {London},
   article_number = {12242},
   doi = {https://doi.org/10.1038/s41598-018-30729-7},
   keywords = {Yersinia enterocolitica; Colicin F-Y},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific reports},
   title = {Colicin F-Y inhibits pathogenic Yersinia enterocolitica in mice},
   volume = {8},
   year = {2018}
}

TY  - JOUR
ID  - 1461576
AU  - Bosák, Juraj - Micenková, Lenka - Hrala, Matěj - Pomorská, Katarína - Bosáková, Michaela - Krejčí, Pavel - Göpfert, Eduard - Faldyna, Martin - Šmajs, David
PY  - 2018
TI  - Colicin F-Y inhibits pathogenic Yersinia enterocolitica in mice
JF  - Scientific reports
VL  - 8
IS  - 12242
SP  - 1-12
EP  - 1-12
PB  - NATURE PUBLISHING GROUP
SN  - 20452322
KW  - Yersinia enterocolitica
KW  - Colicin F-Y
N2  - Yersiniosis belongs to the common foodborne diseases around the world, and frequently manifests as diarrhea that can be treated with probiotics. Colicin F-Y is an antibacterial agent produced by bacteria and it is capable of specific growth inhibition of Yersinia enterocolitica, the causative agent of gastrointestinal yersiniosis. In this study, recombinant E. coli producing colicin F-Y were constructed, using both known probiotic strains EcH22 and EcColinfant, and the newly isolated murine strains Ec1127 and Ec1145. All E. coli strains producing colicin FY inhibited growth of pathogenic Y. enterocolitica during co-cultivation in vitro. In dysbiotic mice treated with streptomycin, E. coli strains producing colicin F-Y inhibited progression of Y. enterocolitica infections. This growth inhibition was not observed in mice with normal gut microflora, likely due to insufficient colonization capacity of E. coli strains and/or due to spatial differences in intestinal niches. Isogenic Y. enterocolitica producing colicin F-Y was constructed and shown to inhibit pathogenic Y. enterocolitica in mice with normal microflora. Evidence of in vivo antimicrobial activity of colicin F-Y may have utility in the treatment of Y. enterocolitica infections.
ER  -

BOSÁK, Juraj; Lenka MICENKOVÁ; Matěj HRALA; Katarína POMORSKÁ; Michaela BOSÁKOVÁ; Pavel KREJČÍ; Eduard GÖPFERT; Martin FALDYNA a David ŠMAJS. Colicin F-Y inhibits pathogenic Yersinia enterocolitica in mice. \textit{Scientific reports}. London: NATURE PUBLISHING GROUP, 2018, roč.~8, č.~12242, s.~1-12. ISSN~2045-2322. Dostupné z: https://doi.org/10.1038/s41598-018-30729-7.

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