Effect of jasplakinolide and cytochalasin D on cortical
elements involved in the gliding motility of the eugregarine
Gregarina garnhami (Apicomplexa)

J 2018

Effect of jasplakinolide and cytochalasin D on cortical elements involved in the gliding motility of the eugregarine Gregarina garnhami (Apicomplexa)

KOVÁČIKOVÁ, Magdaléna; Naděžda VAŠKOVICOVÁ; Jana NEBESÁŘOVÁ a Andrea BARDŮNEK VALIGUROVÁ

Základní údaje

Originální název

Effect of jasplakinolide and cytochalasin D on cortical elements involved in the gliding motility of the eugregarine Gregarina garnhami (Apicomplexa)

Autoři

KOVÁČIKOVÁ, Magdaléna; Naděžda VAŠKOVICOVÁ; Jana NEBESÁŘOVÁ a Andrea BARDŮNEK VALIGUROVÁ

Vydání

European Journal of Protistology, JENA, ELSEVIER GMBH, 2018, 0932-4739

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.626

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/18:00104436

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Ectoplasmic network; F-actin; Gregarine; Motility; Myonemes; Ultrastructure

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 4. 2024 12:40, Mgr. Michal Petr

Anotace

V originále

Since apicomplexans represent exclusively parasitic unicellular organisms with medical and economic impacts, the principles of their motility have been studied intensively. By contrast, the movement in apicomplexan basal groups, such as gregarines, remains to be elucidated. The present study focuses on Gregarina garnhami parasitising the digestive tract of the locust Schistocerca gregaria, and investigates the involvement of cytoskeletal elements (the ectoplasmic network and myonemes) and the secretion of mucosubstances during eugregarine gliding motility. Combined microscopic analyses were used to verify the role of actin filaments and membranes' organisation in G. garnhami motility. A freeze-etching analysis of membranes revealed the size, density, and arrangement of intramembranous particles along with the distribution and size of pores and ducts. Experimental assays using actin-modifying drugs (jasplakinolide, cytochalasin D) confirmed that actin most likely plays a role in cell motility, principally in its filamentous form (=F-actin). Myonemes, localised in the border between the ectoplasm and endoplasm, correspond to the concentric bundles of F-actin. Microscopic analyses confirmed that changes in gamonts motility corresponding to the changes in the organisation and density of myonemes and the ectoplasmic network in drug-treated cells, suggesting that these structures might serve as contractile elements facilitating gliding motility in G. garnhami.

Citovat

KOVÁČIKOVÁ, Magdaléna; Naděžda VAŠKOVICOVÁ; Jana NEBESÁŘOVÁ a Andrea BARDŮNEK VALIGUROVÁ. Effect of jasplakinolide and cytochalasin D on cortical elements involved in the gliding motility of the eugregarine Gregarina garnhami (Apicomplexa). European Journal of Protistology. JENA: ELSEVIER GMBH, 2018, roč. 66, OCT, s. 97-114. ISSN 0932-4739. Dostupné z: https://doi.org/10.1016/j.ejop.2018.08.006.

@article{1466756,
   author = {Kováčiková, Magdaléna and Vaškovicová, Naděžda and Nebesářová, Jana and Bardůnek Valigurová, Andrea},
   article_location = {JENA},
   article_number = {OCT},
   doi = {https://doi.org/10.1016/j.ejop.2018.08.006},
   keywords = {Ectoplasmic network; F-actin; Gregarine; Motility; Myonemes; Ultrastructure},
   language = {eng},
   issn = {0932-4739},
   journal = {European Journal of Protistology},
   title = {Effect of jasplakinolide and cytochalasin D on cortical elements involved in the gliding motility of the eugregarine Gregarina garnhami (Apicomplexa)},
   url = {https://www.sciencedirect.com/science/article/pii/S0932473918300403},
   volume = {66},
   year = {2018}
}

TY  - JOUR
ID  - 1466756
AU  - Kováčiková, Magdaléna - Vaškovicová, Naděžda - Nebesářová, Jana - Bardůnek Valigurová, Andrea
PY  - 2018
TI  - Effect of jasplakinolide and cytochalasin D on cortical elements involved in the gliding motility of the eugregarine Gregarina garnhami (Apicomplexa)
JF  - European Journal of Protistology
VL  - 66
IS  - OCT
SP  - 97-114
EP  - 97-114
PB  - ELSEVIER GMBH
SN  - 09324739
KW  - Ectoplasmic network
KW  - F-actin
KW  - Gregarine
KW  - Motility
KW  - Myonemes
KW  - Ultrastructure
UR  - https://www.sciencedirect.com/science/article/pii/S0932473918300403
N2  - Since apicomplexans represent exclusively parasitic unicellular organisms with medical and economic impacts, the principles of their motility have been studied intensively. By contrast, the movement in apicomplexan basal groups, such as gregarines, remains to be elucidated. The present study focuses on Gregarina garnhami parasitising the digestive tract of the locust Schistocerca gregaria, and investigates the involvement of cytoskeletal elements (the ectoplasmic network and myonemes) and the secretion of mucosubstances during eugregarine gliding motility. Combined microscopic analyses were used to verify the role of actin filaments and membranes' organisation in G. garnhami motility. A freeze-etching analysis of membranes revealed the size, density, and arrangement of intramembranous particles along with the distribution and size of pores and ducts. Experimental assays using actin-modifying drugs (jasplakinolide, cytochalasin D) confirmed that actin most likely plays a role in cell motility, principally in its filamentous form (=F-actin). Myonemes, localised in the border between the ectoplasm and endoplasm, correspond to the concentric bundles of F-actin. Microscopic analyses confirmed that changes in gamonts motility corresponding to the changes in the organisation and density of myonemes and the ectoplasmic network in drug-treated cells, suggesting that these structures might serve as contractile elements facilitating gliding motility in G. garnhami.
ER  -

KOVÁČIKOVÁ, Magdaléna; Naděžda VAŠKOVICOVÁ; Jana NEBESÁŘOVÁ a Andrea BARDŮNEK VALIGUROVÁ. Effect of jasplakinolide and cytochalasin D on cortical elements involved in the gliding motility of the eugregarine Gregarina garnhami (Apicomplexa). \textit{European Journal of Protistology}. JENA: ELSEVIER GMBH, 2018, roč.~66, OCT, s.~97-114. ISSN~0932-4739. Dostupné z: https://doi.org/10.1016/j.ejop.2018.08.006.

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