High-Resolution NMR of Folded Proteins in Hyperpolarized
Physiological Solvents

J 2018

High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents

KADEŘÁVEK, Pavel; F. FERRAGE; G. BODENHAUSEN a D. KURZBACH

Základní údaje

Originální název

High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents

Autoři

KADEŘÁVEK, Pavel ; F. FERRAGE; G. BODENHAUSEN a D. KURZBACH

Vydání

Chemistry - A European Journal, WEINHEIM, Wiley, 2018, 0947-6539

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10402 Inorganic and nuclear chemistry

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.160

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/18:00104510

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

dissolution DNP; hyperpolarized water; cross-relaxation effect; protein NMR spectroscopy; proton exchange

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 3. 2019 11:20, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Hyperpolarized 2D exchange spectroscopy (HYPEX) to obtain high-resolution nuclear magnetic resonance (NMR) spectra of folded proteins under near-physiological conditions is reported. The technique is based on hyperpolarized water, which is prepared by dissolution dynamic nuclear polarization and mixed in situ in an NMR spectrometer with a protein in a physiological saline buffer at body temperature. Rapid exchange of labile protons with the hyperpolarized solvent, combined with cross-relaxation effects (NOEs), leads to boosted signal intensities for many amide H-1-N-15 correlations in the protein ubiquitin. As the introduction of hyperpolarization to the target protein is mediated via the solvent, the method is applicable to a broad spectrum of target molecules.

Citovat

KADEŘÁVEK, Pavel; F. FERRAGE; G. BODENHAUSEN a D. KURZBACH. High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents. Chemistry - A European Journal. WEINHEIM: Wiley, 2018, roč. 24, č. 51, s. 13418-13423. ISSN 0947-6539. Dostupné z: https://doi.org/10.1002/chem.201802885.

@article{1468956,
   author = {Kadeřávek, Pavel and Ferrage, F. and Bodenhausen, G. and Kurzbach, D.},
   article_location = {WEINHEIM},
   article_number = {51},
   doi = {https://doi.org/10.1002/chem.201802885},
   keywords = {dissolution DNP; hyperpolarized water; cross-relaxation effect; protein NMR spectroscopy; proton exchange},
   language = {eng},
   issn = {0947-6539},
   journal = {Chemistry - A European Journal},
   title = {High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents},
   url = {https://onlinelibrary.wiley.com/doi/full/10.1002/chem.201802885},
   volume = {24},
   year = {2018}
}

TY  - JOUR
ID  - 1468956
AU  - Kadeřávek, Pavel - Ferrage, F. - Bodenhausen, G. - Kurzbach, D.
PY  - 2018
TI  - High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents
JF  - Chemistry - A European Journal
VL  - 24
IS  - 51
SP  - 13418-13423
EP  - 13418-13423
PB  - Wiley
SN  - 09476539
KW  - dissolution DNP
KW  - hyperpolarized water
KW  - cross-relaxation effect
KW  - protein NMR spectroscopy
KW  - proton exchange
UR  - https://onlinelibrary.wiley.com/doi/full/10.1002/chem.201802885
N2  - Hyperpolarized 2D exchange spectroscopy (HYPEX) to obtain high-resolution nuclear magnetic resonance (NMR) spectra of folded proteins under near-physiological conditions is reported. The technique is based on hyperpolarized water, which is prepared by dissolution dynamic nuclear polarization and mixed in situ in an NMR spectrometer with a protein in a physiological saline buffer at body temperature. Rapid exchange of labile protons with the hyperpolarized solvent, combined with cross-relaxation effects (NOEs), leads to boosted signal intensities for many amide H-1-N-15 correlations in the protein ubiquitin. As the introduction of hyperpolarization to the target protein is mediated via the solvent, the method is applicable to a broad spectrum of target molecules.
ER  -

KADEŘÁVEK, Pavel; F. FERRAGE; G. BODENHAUSEN a D. KURZBACH. High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents. \textit{Chemistry - A European Journal}. WEINHEIM: Wiley, 2018, roč.~24, č.~51, s.~13418-13423. ISSN~0947-6539. Dostupné z: https://doi.org/10.1002/chem.201802885.

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