Capillary Blood GSH Level Monitoring, Using an Electrochemical
Method Adapted for Micro Volumes

J 2018

Capillary Blood GSH Level Monitoring, Using an Electrochemical Method Adapted for Micro Volumes

BUCHTOVÁ, Žaneta; Zuzana LACKOVÁ; Jiří KUDR; Jan ŠKODA; Ondřej ZÍTKA et al.

Základní údaje

Originální název

Capillary Blood GSH Level Monitoring, Using an Electrochemical Method Adapted for Micro Volumes

Autoři

BUCHTOVÁ, Žaneta; Zuzana LACKOVÁ; Jiří KUDR; Jan ŠKODA; Ondřej ZÍTKA a Zdeněk ZÍTKA

Vydání

Molecules, Mayer und Muller, 2018, 1420-3049

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.060

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14510/18:00120408

Organizační jednotka

Fakulta sportovních studií

Klíčová slova anglicky

antioxidant molecules; blood drop analysis; electrochemical analysis; nutritional study; sample pretreatment

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 5. 2022 14:19, Mgr. Pavlína Roučová, DiS.

Anotace

V originále

Glutathione ( -glutamyl-cysteinyl-glycine; also known as GSH) is an endogenous antioxidant that plays a crucial role in cell defense mechanisms against oxidative stress. It is thus not surprising that this molecule can serve as a biomarker for oxidative stress monitoring. As capillary blood is a highly accessible target for biomarking, it is a valuable bodily fluid for diagnosing human GSH levels. This study focused on the optimization of GSH measurements from micro volumes of capillary blood prior to using electrochemical detection. The optimization of experimental parameters, including the sample volume and its stability, was performed and evaluated. Moreover, we tested the optimized method as part of a short-term study. The study consisted of examining 10 subjects within 96 h of their consumption of high amounts of antioxidants, attained from a daily dose of 2 g/150 mL of green tea. The subjects’ capillary blood (5 L) was taken at 0 h, 48 h, and 96 h for subsequent analysis. The short-term supplementation of diet with green tea

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

BUCHTOVÁ, Žaneta; Zuzana LACKOVÁ; Jiří KUDR; Jan ŠKODA; Ondřej ZÍTKA a Zdeněk ZÍTKA. Capillary Blood GSH Level Monitoring, Using an Electrochemical Method Adapted for Micro Volumes. Molecules. Mayer und Muller, 2018, roč. 23, říjen 2018, s. 1-10. ISSN 1420-3049. Dostupné z: https://doi.org/10.3390/molecules23102504.

@article{1471036,
   author = {Buchtová, Žaneta and Lacková, Zuzana and Kudr, Jiří and Škoda, Jan and Zítka, Ondřej and Zítka, Zdeněk},
   article_number = {říjen 2018},
   doi = {https://doi.org/10.3390/molecules23102504},
   keywords = {antioxidant molecules; blood drop analysis; electrochemical analysis; nutritional study; sample pretreatment},
   language = {eng},
   issn = {1420-3049},
   journal = {Molecules},
   title = {Capillary Blood GSH Level Monitoring, Using an Electrochemical Method Adapted for Micro Volumes},
   url = {https://www.mdpi.com/1420-3049/23/10/2504},
   volume = {23},
   year = {2018}
}

TY  - JOUR
ID  - 1471036
AU  - Buchtová, Žaneta - Lacková, Zuzana - Kudr, Jiří - Škoda, Jan - Zítka, Ondřej - Zítka, Zdeněk
PY  - 2018
TI  - Capillary Blood GSH Level Monitoring, Using an Electrochemical Method Adapted for Micro Volumes
JF  - Molecules
VL  - 23
IS  - říjen 2018
SP  - 1-10
EP  - 1-10
PB  - Mayer und Muller
SN  - 14203049
KW  - antioxidant molecules
KW  - blood drop analysis
KW  - electrochemical analysis
KW  - nutritional study
KW  - sample pretreatment
UR  - https://www.mdpi.com/1420-3049/23/10/2504
L2  - https://www.mdpi.com/1420-3049/23/10/2504
N2  - Glutathione ( -glutamyl-cysteinyl-glycine; also known as GSH) is an endogenous antioxidant that plays a crucial role in cell defense mechanisms against oxidative stress. It is thus not surprising that this molecule can serve as a biomarker for oxidative stress monitoring. As capillary blood is a highly accessible target for biomarking, it is a valuable bodily fluid for diagnosing human GSH levels. This study focused on the optimization of GSH measurements from micro volumes of capillary blood prior to using electrochemical detection. The optimization of experimental parameters, including the sample volume and its stability, was performed and evaluated. Moreover, we tested the optimized method as part of a short-term study. The study consisted of examining 10 subjects within 96 h of their consumption of high amounts of antioxidants, attained from a daily dose of 2 g/150 mL of green tea. The subjects’ capillary blood (5 L) was taken at 0 h, 48 h, and 96 h for subsequent analysis. The short-term supplementation of diet with green tea
ER  -

BUCHTOVÁ, Žaneta; Zuzana LACKOVÁ; Jiří KUDR; Jan ŠKODA; Ondřej ZÍTKA a Zdeněk ZÍTKA. Capillary Blood GSH Level Monitoring, Using an Electrochemical Method Adapted for Micro Volumes. \textit{Molecules}. Mayer und Muller, 2018, roč.~23, říjen 2018, s.~1-10. ISSN~1420-3049. Dostupné z: https://doi.org/10.3390/molecules23102504.

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