Reversible method for assessment of tubular membrane
capacitance in cardiomyocytes

a 2018

Reversible method for assessment of tubular membrane capacitance in cardiomyocytes

ŠVECOVÁ, Olga; Markéta BÉBAROVÁ; Milena ŠIMURDOVÁ a Jiří ŠIMURDA

Základní údaje

Originální název

Reversible method for assessment of tubular membrane capacitance in cardiomyocytes

Autoři

ŠVECOVÁ, Olga ; Markéta BÉBAROVÁ ; Milena ŠIMURDOVÁ a Jiří ŠIMURDA

Vydání

New Frontiers in Basic Cardiovascular Research: France - New EU Members, 2018

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

Příznaky

Mezinárodní význam

Změněno: 26. 11. 2018 17:10, doc. MUDr. Markéta Bébarová, Ph.D.

Anotace

V originále

Transverse-axial tubular system (TATS) plays a key role in the cardiac cell function. Some cardiac diseases (e.g. heart failure) lead to structural and functional changes in TATS. Among others, the membrane capacitance of TATS may be altered. The TATS and surface membranes are closely interconnected and traditional methods of assessment of the cell membrane capacitance not able to distinguish them. Hence, the capacitance of TATS has been determined mainly by using technique called detubulation which is, however, irreversible and shows several important limitations. We have recently developed a new method for estimation of TATS capacitance in isolated cardiomyocytes that is fully reversible. The method is based on electrical separation of the surface and TATS membranes. It uses short applications of isotonic sucrose (IS) solution which leads to a significant increase of the electric resistance of TATS. The electrical parameters of the two membrane systems could be then analysed separately. After application of IS solution, its diffusion into TATS lead to transient changes in the membrane current and potential. These changes were well reproduced by model simulations. Capacities of TATS and surface membranes were determined from approximation of the current responses by a sum of two exponential functions. Several criteria for selection of reliable data were looked for and defined. Based on the data and their subsequent analysis, we conclude that the fractional value of TATS capacitance in adult rat ventricular myocytes is ~0.43 on average.

Návaznosti

NV16-30571A, projekt VaV

Název: Klinický význam a elektrofyziologické zhodnocení mutace c.926C>T genu KCNQ1 (p.T309I) jako možné „founder mutation“ syndromu dlouhého intervalu QT

Citovat

ŠVECOVÁ, Olga; Markéta BÉBAROVÁ; Milena ŠIMURDOVÁ a Jiří ŠIMURDA. Reversible method for assessment of tubular membrane capacitance in cardiomyocytes. In New Frontiers in Basic Cardiovascular Research: France - New EU Members. 2018.

@proceedings{1472417,
   author = {Švecová, Olga and Bébarová, Markéta and Šimurdová, Milena and Šimurda, Jiří},
   booktitle = {New Frontiers in Basic Cardiovascular Research: France - New EU Members},
   language = {eng},
   title = {Reversible method for assessment of tubular membrane capacitance in cardiomyocytes},
   year = {2018}
}

TY  - CONF
ID  - 1472417
AU  - Švecová, Olga - Bébarová, Markéta - Šimurdová, Milena - Šimurda, Jiří
PY  - 2018
TI  - Reversible method for assessment of tubular membrane capacitance in cardiomyocytes
N2  - Transverse-axial tubular system (TATS) plays a key role in the cardiac cell function. Some cardiac diseases (e.g. heart failure) lead to structural and functional changes in TATS. Among others, the membrane capacitance of TATS may be altered. The TATS and surface membranes are closely interconnected and traditional methods of assessment of the cell membrane capacitance not able to distinguish them. Hence, the capacitance of TATS has been determined mainly by using technique called detubulation which is, however, irreversible and shows several important limitations. We have recently developed a new method for estimation of TATS capacitance in isolated cardiomyocytes that is fully reversible. The method is based on electrical separation of the surface and TATS membranes. It uses short applications of isotonic sucrose (IS) solution which leads to a significant increase of the electric resistance of TATS. The electrical parameters of the two membrane systems could be then analysed separately. After application of IS solution, its diffusion into TATS lead to transient changes in the membrane current and potential. These changes were well reproduced by model simulations. Capacities of TATS and surface membranes were determined from approximation of the current responses by a sum of two exponential functions. Several criteria for selection of reliable data were looked for and defined. Based on the data and their subsequent analysis, we conclude that the fractional value of TATS capacitance in adult rat ventricular myocytes is ~0.43 on average.
ER  -

ŠVECOVÁ, Olga; Markéta BÉBAROVÁ; Milena ŠIMURDOVÁ a Jiří ŠIMURDA. Reversible method for assessment of tubular membrane capacitance in cardiomyocytes. In \textit{New Frontiers in Basic Cardiovascular Research: France - New EU Members}. 2018.

Přehled o publikaci