CAPILLARY ELECTROPHORETIC ANALYSIS OF EXHALED BREATH CONDENSATE
IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE

J 2018

CAPILLARY ELECTROPHORETIC ANALYSIS OF EXHALED BREATH CONDENSATE IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE

KUBÁŇ, Petr; Pavol ĎURČ; Júlia LAČNÁ; Michal GREGUŠ; František FORET et al.

Basic information

Original name

CAPILLARY ELECTROPHORETIC ANALYSIS OF EXHALED BREATH CONDENSATE IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE

Authors

KUBÁŇ, Petr; Pavol ĎURČ; Júlia LAČNÁ; Michal GREGUŠ; František FORET; Jiří DOLINA; Štefan KONEČNÝ; Martina DOUBKOVÁ; Dagmar KINDLOVÁ; Eva POKOJOVÁ and Jana SKŘIČKOVÁ

Edition

HUNGARIAN JOURNAL OF INDUSTRY AND CHEMISTRY, WARSAW, DE GRUYTER POLAND SP ZOO, 2018, 0133-0276

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10406 Analytical chemistry

Country of publisher

Poland

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 0.084 in 2002

Marked to be transferred to RIV

Yes

RIV identification code

RIV/00216224:14740/18:00106949

Organization unit

Central European Institute of Technology

DOI

https://doi.org/10.1515/hjic-2018-0006

UT WoS

000440079700006

Keywords in English

capillary electrophoresis; exhaled breath condensate; ionic analysis; diagnosis; gastroesophageal reflux disease

Abstract

In the original language

In this work, capillary electrophoresis with contactless conductometric detection (CCD) was used for the analysis of the ionic content of exhaled breath condensate (EBC) to differentiate between healthy individuals and patients with gastroesophageal reflux disease (GERD). The exhaled breath condensate was collected using a miniature sample collection device and the content analyzed using a separation electrolyte composed of 20 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM L-histidine, 2 mM 18-Crown-6 and 30 mu M cetyltrimethylammonium bromide. The separation of anions took less than 2.5 minutes, while the cations were separated in less than 1.5 minutes. The most significantly elevated ions in the group of patients suffering from gastroesophageal reflux disease were chloride, nitrate, propionate and butyrate. Although the number of subjects was too small to draw definite conclusions with regard to the discriminatory power of these ions, the pilot data are promising for EBC as a useful non-invasive alternative for other methods used in the diagnosis of gastroesophageal reflux disease.

Links

NV17-31945A, research and development project

Name: Vývoj neinvazivní diagnostiky extraezofageálních projevů refluxní choroby jícnu

Cite

KUBÁŇ, Petr; Pavol ĎURČ; Júlia LAČNÁ; Michal GREGUŠ; František FORET; Jiří DOLINA; Štefan KONEČNÝ; Martina DOUBKOVÁ; Dagmar KINDLOVÁ; Eva POKOJOVÁ and Jana SKŘIČKOVÁ. CAPILLARY ELECTROPHORETIC ANALYSIS OF EXHALED BREATH CONDENSATE IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE. HUNGARIAN JOURNAL OF INDUSTRY AND CHEMISTRY. WARSAW: DE GRUYTER POLAND SP ZOO, 2018, vol. 46, No 1, p. 23-27. ISSN 0133-0276. Available from: https://doi.org/10.1515/hjic-2018-0006.

@article{1472910,
   author = {Kubáň, Petr and Ďurč, Pavol and Lačná, Júlia and Greguš, Michal and Foret, František and Dolina, Jiří and Konečný, Štefan and Doubková, Martina and Kindlová, Dagmar and Pokojová, Eva and Skřičková, Jana},
   article_location = {WARSAW},
   article_number = {1},
   doi = {https://doi.org/10.1515/hjic-2018-0006},
   keywords = {capillary electrophoresis; exhaled breath condensate; ionic analysis; diagnosis; gastroesophageal reflux disease},
   language = {eng},
   issn = {0133-0276},
   journal = {HUNGARIAN JOURNAL OF INDUSTRY AND CHEMISTRY},
   title = {CAPILLARY ELECTROPHORETIC ANALYSIS OF EXHALED BREATH CONDENSATE IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE},
   url = {https://content.sciendo.com/view/journals/hjic/46/1/article-p23.xml},
   volume = {46},
   year = {2018}
}

TY  - JOUR
ID  - 1472910
AU  - Kubáň, Petr - Ďurč, Pavol - Lačná, Júlia - Greguš, Michal - Foret, František - Dolina, Jiří - Konečný, Štefan - Doubková, Martina - Kindlová, Dagmar - Pokojová, Eva - Skřičková, Jana
PY  - 2018
TI  - CAPILLARY ELECTROPHORETIC ANALYSIS OF EXHALED BREATH CONDENSATE IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE
JF  - HUNGARIAN JOURNAL OF INDUSTRY AND CHEMISTRY
VL  - 46
IS  - 1
SP  - 23-27
EP  - 23-27
PB  - DE GRUYTER POLAND SP ZOO
SN  - 01330276
KW  - capillary electrophoresis
KW  - exhaled breath condensate
KW  - ionic analysis
KW  - diagnosis
KW  - gastroesophageal reflux disease
UR  - https://content.sciendo.com/view/journals/hjic/46/1/article-p23.xml
N2  - In this work, capillary electrophoresis with contactless conductometric detection (CCD) was used for the analysis of the ionic content of exhaled breath condensate (EBC) to differentiate between healthy individuals and patients with gastroesophageal reflux disease (GERD). The exhaled breath condensate was collected using a miniature sample collection device and the content analyzed using a separation electrolyte composed of 20 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM L-histidine, 2 mM 18-Crown-6 and 30 mu M cetyltrimethylammonium bromide. The separation of anions took less than 2.5 minutes, while the cations were separated in less than 1.5 minutes. The most significantly elevated ions in the group of patients suffering from gastroesophageal reflux disease were chloride, nitrate, propionate and butyrate. Although the number of subjects was too small to draw definite conclusions with regard to the discriminatory power of these ions, the pilot data are promising for EBC as a useful non-invasive alternative for other methods used in the diagnosis of gastroesophageal reflux disease.
ER  -

KUBÁŇ, Petr; Pavol ĎURČ; Júlia LAČNÁ; Michal GREGUŠ; František FORET; Jiří DOLINA; Štefan KONEČNÝ; Martina DOUBKOVÁ; Dagmar KINDLOVÁ; Eva POKOJOVÁ and Jana SKŘIČKOVÁ. CAPILLARY ELECTROPHORETIC ANALYSIS OF EXHALED BREATH CONDENSATE IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE. \textit{HUNGARIAN JOURNAL OF INDUSTRY AND CHEMISTRY}. WARSAW: DE GRUYTER POLAND SP ZOO, 2018, vol.~46, No~1, p.~23-27. ISSN~0133-0276. Available from: https://doi.org/10.1515/hjic-2018-0006.

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