AB21, a protein of unknown function from Agaricus bisporus. A
case study on utilizing experimental and bioinformatical
approaches for functional assignment of unknown proteins

a 2018

AB21, a protein of unknown function from Agaricus bisporus. A case study on utilizing experimental and bioinformatical approaches for functional assignment of unknown proteins

KOMÁREK, Jan; Eva KAVKOVÁ; Josef HOUSER; Aneta HORÁČKOVÁ; Jitka HOLKOVÁ et. al.

Základní údaje

Originální název

AB21, a protein of unknown function from Agaricus bisporus. A case study on utilizing experimental and bioinformatical approaches for functional assignment of unknown proteins

Autoři

KOMÁREK, Jan (203 Česká republika, domácí); Eva KAVKOVÁ (203 Česká republika, domácí); Josef HOUSER (203 Česká republika, domácí); Aneta HORÁČKOVÁ (203 Česká republika, domácí); Jitka HOLKOVÁ (203 Česká republika, domácí); Gabriel DEMO (703 Slovensko, domácí) a Michaela WIMMEROVÁ (203 Česká republika, garant, domácí)

Vydání

XIX. Setkání biochemiků a molekulárních biologů, 2018

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/18:00104707

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-210-9069-9

Klíčová slova česky

proteiny neznámé funkce Agaricus bisporus,

Klíčová slova anglicky

proteins of unknown function; Agaricus bisporus

Změněno: 29. 11. 2018 16:20, Mgr. Jan Komárek, Ph.D.

Anotace

V originále

Progress in genome sequencing over the last two decades has led to the accumulation of a huge number of sequences in the public databases (currently over 126 million of protein entries). However, a significant fraction of them represents proteins that have not been experimentally characterized, and whose functions cannot be deduced from the sequence comparisons. Investigating the functions and properties of unknown proteins is therefore an important and challenging task to understanding the whole picture of processes that occur in living organisms, but also an opportunity to discover new aspects of life´s machinery. This work is focused on protein AB21 from the white button mushroom (Agaricus bisporus), one of the most widely cultivated mushroom in the world. AB21 is a 21 kDa protein, which shares no detectable sequence homology to any protein of known function. Hence, this work can be considered as a case study on utilizing different biophysical, structural and bioinformatical methods to investigate its possible biological role. The coding sequence of ab21 gene was determined and the gene was expressed in a recombinant form in E. coli. AB21 was characterized in terms of quaternary structure, stability and possible interactions with physiologically relevant ligands. The determined X ray structure of AB21 exhibited an interesting relation to bacterial pore-forming toxins of the ClyA superfamily. Finally, the naturally occuring protein was immunolocalized within the fruiting bodies of A. bisporus.

Návaznosti

LM2015043, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

LTC17076, projekt VaV

Název: Interdisciplinární přístup ke studiu biologických systémů na molekulární úrovni

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interdisciplinární přístup ke studiu biologických systémů na molekulární úrovni, INTER-COST

Citovat

KOMÁREK, Jan; Eva KAVKOVÁ; Josef HOUSER; Aneta HORÁČKOVÁ; Jitka HOLKOVÁ; Gabriel DEMO a Michaela WIMMEROVÁ. AB21, a protein of unknown function from Agaricus bisporus. A case study on utilizing experimental and bioinformatical approaches for functional assignment of unknown proteins. In XIX. Setkání biochemiků a molekulárních biologů. 2018. ISBN 978-80-210-9069-9.

@proceedings{1473303,
   author = {Komárek, Jan and Kavková, Eva and Houser, Josef and Horáčková, Aneta and Holková, Jitka and Demo, Gabriel and Wimmerová, Michaela},
   booktitle = {XIX. Setkání biochemiků a molekulárních biologů},
   keywords = {proteins of unknown function; Agaricus bisporus},
   language = {eng},
   isbn = {978-80-210-9069-9},
   title = {AB21, a protein of unknown function from Agaricus bisporus. A case study on utilizing experimental and bioinformatical approaches for functional assignment of unknown proteins},
   year = {2018}
}

TY  - CONF
ID  - 1473303
AU  - Komárek, Jan - Kavková, Eva - Houser, Josef - Horáčková, Aneta - Holková, Jitka - Demo, Gabriel - Wimmerová, Michaela
PY  - 2018
TI  - AB21, a protein of unknown function from Agaricus bisporus. A case study on utilizing experimental and bioinformatical approaches for functional assignment of unknown proteins
SN  - 9788021090699
KW  - proteins of unknown function
KW  - Agaricus bisporus
N2  - Progress in genome sequencing over the last two decades has led to the accumulation of a huge number of sequences in the public databases (currently over 126 million of protein entries). However, a significant fraction of them represents proteins that have not been experimentally characterized, and whose functions cannot be deduced from the sequence comparisons. Investigating the functions and properties of unknown proteins is therefore an important and challenging task to understanding the whole picture of processes that occur in living organisms, but also an opportunity to discover new aspects of life´s machinery. This work is focused on protein AB21 from the white button mushroom (Agaricus bisporus), one of the most widely cultivated mushroom in the world. AB21 is a 21 kDa protein, which shares no detectable sequence homology to any protein of known function. Hence, this work can be considered as a case study on utilizing different biophysical, structural and bioinformatical methods to investigate its possible biological role. The coding sequence of ab21 gene was determined and the gene was expressed in a recombinant form in E. coli. AB21 was characterized in terms of quaternary structure, stability and possible interactions with physiologically relevant ligands. The determined X ray structure of AB21 exhibited an interesting relation to bacterial pore-forming toxins of the ClyA superfamily. Finally, the naturally occuring protein was immunolocalized within the fruiting bodies of A. bisporus.
ER  -

KOMÁREK, Jan; Eva KAVKOVÁ; Josef HOUSER; Aneta HORÁČKOVÁ; Jitka HOLKOVÁ; Gabriel DEMO a Michaela WIMMEROVÁ. AB21, a protein of unknown function from Agaricus bisporus. A case study on utilizing experimental and bioinformatical approaches for functional assignment of unknown proteins. In \textit{XIX. Setkání biochemiků a molekulárních biologů}. 2018. ISBN~978-80-210-9069-9.

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