Voltammetric and adsorption study of
4-nitrophenyl-triazole-labeled 2 '-deoxycytidine and
7-deazaadenosine nucleosides at boron-doped diamond electrode

J 2018

Voltammetric and adsorption study of 4-nitrophenyl-triazole-labeled 2 '-deoxycytidine and 7-deazaadenosine nucleosides at boron-doped diamond electrode

VOSAHLOVA, J.; L. KOLACNA; A. DANHEL; J. FISCHER; J. BALINTOVA et al.

Základní údaje

Originální název

Voltammetric and adsorption study of 4-nitrophenyl-triazole-labeled 2 '-deoxycytidine and 7-deazaadenosine nucleosides at boron-doped diamond electrode

Autoři

VOSAHLOVA, J.; L. KOLACNA; A. DANHEL; J. FISCHER; J. BALINTOVA; M. HOCEK; K. SCHWARZOVA-PECKOVA a Miroslav FOJTA

Vydání

Journal of Electroanalytical Chemistry, Lausanne, Elsevier, 2018, 1572-6657

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.218

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/18:00101395

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Adsorption; Boron-doped diamond; Nitro compounds; Nucleoside labeling; Voltammetry

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 3. 2019 17:28, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Electrochemical properties of 4-nitrophenyl triazole labeled 2'-deoxycytidine (dCTRN 2) and 7-deazaadenosine (dA(TRNO2)) were studied using alumina-polished boron doped diamond (BDD) electrode in acetate buffer pH 5.0. Cyclic voltammetry revealed peak-shaped signals for reduction of the nitro group at the potentials of ca -0.6 V (vs. AllAgCl13 mol L-1 KCl reference electrode) and for the hydroxylamine/nitroso pair exhibiting quasireversible electron transfer at potentials comparable with redox processes of other nitro aromatic compounds at BDD electrodes. All electrochemical processes are directed by adsorption. Moreover, ex situ adsorption of dA(TRNO2) and transfer to supporting electrolyte enables its quantitation using square wave voltammetry in small (several microliter) sample volumes after optimization of parameters for the hydroxylamine/nitroso redox couple. Competitive adsorption/desorption at the BDD surface was further studied by cyclic voltammetry with dA(TRNO2) in the presence of electrochemically inactive phenyl triazole 7-deazaadenosine conjugate (dA(TR)), revealing mutual displacement of both compounds at BDD surface. Further, the redox processes of simpler aromatic nitro compounds (4-nitrophenyl acetylene and 2-nitronaphthalene) are strongly affected when the BDD surface is covered by dA(TR).

Návaznosti

GBP206/12/G151, projekt VaV

Název: Centrum nových přístupů k bioanalýze a molekulární diagnostice

Citovat

VOSAHLOVA, J.; L. KOLACNA; A. DANHEL; J. FISCHER; J. BALINTOVA; M. HOCEK; K. SCHWARZOVA-PECKOVA a Miroslav FOJTA. Voltammetric and adsorption study of 4-nitrophenyl-triazole-labeled 2 '-deoxycytidine and 7-deazaadenosine nucleosides at boron-doped diamond electrode. Journal of Electroanalytical Chemistry. Lausanne: Elsevier, 2018, roč. 821, JUL, s. 111-120. ISSN 1572-6657. Dostupné z: https://doi.org/10.1016/j.jelechem.2018.01.003.

@article{1474663,
   author = {Vosahlova, J. and Kolacna, L. and Danhel, A. and Fischer, J. and Balintova, J. and Hocek, M. and SchwarzovaandPeckova, K. and Fojta, Miroslav},
   article_location = {Lausanne},
   article_number = {JUL},
   doi = {https://doi.org/10.1016/j.jelechem.2018.01.003},
   keywords = {Adsorption; Boron-doped diamond; Nitro compounds; Nucleoside labeling; Voltammetry},
   language = {eng},
   issn = {1572-6657},
   journal = {Journal of Electroanalytical Chemistry},
   title = {Voltammetric and adsorption study of 4-nitrophenyl-triazole-labeled 2 '-deoxycytidine and 7-deazaadenosine nucleosides at boron-doped diamond electrode},
   volume = {821},
   year = {2018}
}

TY  - JOUR
ID  - 1474663
AU  - Vosahlova, J. - Kolacna, L. - Danhel, A. - Fischer, J. - Balintova, J. - Hocek, M. - Schwarzova-Peckova, K. - Fojta, Miroslav
PY  - 2018
TI  - Voltammetric and adsorption study of 4-nitrophenyl-triazole-labeled 2 '-deoxycytidine and 7-deazaadenosine nucleosides at boron-doped diamond electrode
JF  - Journal of Electroanalytical Chemistry
VL  - 821
IS  - JUL
SP  - 111-120
EP  - 111-120
PB  - Elsevier
SN  - 15726657
KW  - Adsorption
KW  - Boron-doped diamond
KW  - Nitro compounds
KW  - Nucleoside labeling
KW  - Voltammetry
N2  - Electrochemical properties of 4-nitrophenyl triazole labeled 2'-deoxycytidine (dCTRN 2) and 7-deazaadenosine (dA(TRNO2)) were studied using alumina-polished boron doped diamond (BDD) electrode in acetate buffer pH 5.0. Cyclic voltammetry revealed peak-shaped signals for reduction of the nitro group at the potentials of ca -0.6 V (vs. AllAgCl13 mol L-1 KCl reference electrode) and for the hydroxylamine/nitroso pair exhibiting quasireversible electron transfer at potentials comparable with redox processes of other nitro aromatic compounds at BDD electrodes. All electrochemical processes are directed by adsorption. Moreover, ex situ adsorption of dA(TRNO2) and transfer to supporting electrolyte enables its quantitation using square wave voltammetry in small (several microliter) sample volumes after optimization of parameters for the hydroxylamine/nitroso redox couple. Competitive adsorption/desorption at the BDD surface was further studied by cyclic voltammetry with dA(TRNO2) in the presence of electrochemically inactive phenyl triazole 7-deazaadenosine conjugate (dA(TR)), revealing mutual displacement of both compounds at BDD surface. Further, the redox processes of simpler aromatic nitro compounds (4-nitrophenyl acetylene and 2-nitronaphthalene) are strongly affected when the BDD surface is covered by dA(TR).
ER  -

VOSAHLOVA, J.; L. KOLACNA; A. DANHEL; J. FISCHER; J. BALINTOVA; M. HOCEK; K. SCHWARZOVA-PECKOVA a Miroslav FOJTA. Voltammetric and adsorption study of 4-nitrophenyl-triazole-labeled 2 '-deoxycytidine and 7-deazaadenosine nucleosides at boron-doped diamond electrode. \textit{Journal of Electroanalytical Chemistry}. Lausanne: Elsevier, 2018, roč.~821, JUL, s.~111-120. ISSN~1572-6657. Dostupné z: https://doi.org/10.1016/j.jelechem.2018.01.003.

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