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@article{1486377, author = {Šínová, Romana and Kudová, Jana and Nešporová, Kristina and Sergej, Karel and Šuláková, Romana and Velebný, Vladimír and Kubala, Lukáš and Karel, Sergej and Šuláková, Romana}, article_location = {Hoboken}, article_number = {8}, doi = {http://dx.doi.org/10.1002/jcp.27992}, keywords = {dynorphins; enkephalins; heart regeneration; mouse embryonic stem cells; opioid receptors}, language = {eng}, issn = {0021-9541}, journal = {Journal of cellular physiology}, title = {Opioid receptors and opioid peptides in the cardiomyogenesis of mouse embryonic stem cells}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.27992}, volume = {234}, year = {2019} }
TY - JOUR ID - 1486377 AU - Šínová, Romana - Kudová, Jana - Nešporová, Kristina - Sergej, Karel - Šuláková, Romana - Velebný, Vladimír - Kubala, Lukáš - Karel, Sergej - Šuláková, Romana PY - 2019 TI - Opioid receptors and opioid peptides in the cardiomyogenesis of mouse embryonic stem cells JF - Journal of cellular physiology VL - 234 IS - 8 SP - 13209-13219 EP - 13209-13219 PB - Wiley SN - 00219541 KW - dynorphins KW - enkephalins KW - heart regeneration KW - mouse embryonic stem cells KW - opioid receptors UR - https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.27992 L2 - https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.27992 N2 - The stimulation of myocardium repair is restricted due to the limited understanding of heart regeneration. Interestingly, endogenous opioid peptides such as dynorphins and enkephalins are suggested to support this process. However, the mechanismwhether through the stimulation of the regenerative capacity of cardiac stem cells or through effects on other cell types in the heartis still not completely understood. Thus, a model of the spontaneous cardiomyogenic differentiation of mouse embryonic stem (mES) cells via the formation of embryoid bodies was used to describe changes in the expression and localization of opioid receptors within cells during the differentiation process and the potential of the selected opioid peptides, dynorphin A and B, and methionin-enkephalins and leucin-enkephalins, to modulate cardiomyogenic differentiation in vitro. The expressions of both - and -opioid receptors significantly increased during mES cell differentiation. Moreover, their primary colocalization with the nucleus was followed by their growing presence on the cytoplasmic membrane with increasing mES cell differentiation status. Interestingly, dynorphin B enhanced the downregulation gene expression of Oct4 characteristic of the pluripotent phenotype. Further, dynorphin B also increased cardiomyocyte-specific Nkx2.5 gene expression. However, neither dynorphin A nor methionin-enkephalins and leucin-enkephalins exhibited any significant effects on the course of mES cell differentiation. In conclusion, despite the increased expression of opioid receptors and some enhancement of mES cell differentiation by dynorphin B, the overall data do not support the notion that opioid peptides have a significant potential to promote the spontaneous cardiomyogenesis of mES cells in vitro. ER -
ŠÍNOVÁ, Romana, Jana KUDOVÁ, Kristina NEŠPOROVÁ, Karel SERGEJ, Romana ŠULÁKOVÁ, Vladimír VELEBNÝ a Lukáš KUBALA. Opioid receptors and opioid peptides in the cardiomyogenesis of mouse embryonic stem cells. \textit{Journal of cellular physiology}. Hoboken: Wiley, 2019, roč.~234, č.~8, s.~13209-13219. ISSN~0021-9541. Dostupné z: https://dx.doi.org/10.1002/jcp.27992.
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