| VYCHYTILOVÁ, Petra, Karolína ŠTÍTKOVCOVÁ, Lenka RADOVÁ, Milana ŠACHLOVÁ, Zdeňka HERUDKOVÁ, Kateřina SLABÁ, Zdeněk KALA, Marek SVOBODA, Igor KISS, Rostislav VYZULA, William C. CHO a Ondřej SLABÝ. Circulating PIWI-Interacting RNAs piR-5937 and piR-28876 Are Promising Diagnostic Biomarkers of Colon Cancer. CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION. PHILADELPHIA: AMER ASSOC CANCER RESEARCH, 2018, roč. 27, č. 9, s. 1019-1028. ISSN 1055-9965. Dostupné z: https://dx.doi.org/10.1158/1055-9965.EPI-18-0318. |
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@article{1489418,
author = {Vychytilová, Petra and Štítkovcová, Karolína and Radová, Lenka and Šachlová, Milana and Herudková, Zdeňka and Slabá, Kateřina and Kala, Zdeněk and Svoboda, Marek and Kiss, Igor and Vyzula, Rostislav and Cho, William C. and Slabý, Ondřej},
article_location = {PHILADELPHIA},
article_number = {9},
doi = {http://dx.doi.org/10.1158/1055-9965.EPI-18-0318},
keywords = {Circulating PIWI-interacting RNAs},
language = {eng},
issn = {1055-9965},
journal = {CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION},
title = {Circulating PIWI-Interacting RNAs piR-5937 and piR-28876 Are Promising Diagnostic Biomarkers of Colon Cancer},
volume = {27},
year = {2018}
}
TY - JOUR
ID - 1489418
AU - Vychytilová, Petra - Štítkovcová, Karolína - Radová, Lenka - Šachlová, Milana - Herudková, Zdeňka - Slabá, Kateřina - Kala, Zdeněk - Svoboda, Marek - Kiss, Igor - Vyzula, Rostislav - Cho, William C. - Slabý, Ondřej
PY - 2018
TI - Circulating PIWI-Interacting RNAs piR-5937 and piR-28876 Are Promising Diagnostic Biomarkers of Colon Cancer
JF - CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
VL - 27
IS - 9
SP - 1019-1028
EP - 1019-1028
PB - AMER ASSOC CANCER RESEARCH
SN - 10559965
KW - Circulating PIWI-interacting RNAs
N2 - Background: The early detection of colon cancer is one of the main prerequisites for successful treatment and mortality reduction. Circulating PIWI-interacting RNAs (piRNA) were recently identified as novel promising biomarkers. The purpose of the study was to assess the profiles of piRNAs in blood serum of colon cancer patients with the aim to identify those with high diagnostic potential. Methods: Blood serum samples from 403 colon cancer patients and 276 healthy donors were included in this 3-phase biomarker study. Large-scale piRNA expression profiling was performed using Illumina small RNA sequencing. The diagnostic potential of selected piRNAs was further validated on independent training and validation sets of samples using RT-qPCR. Results: In total, 31 piRNAs were found to be significantly deregulated in serum of cancer patients compared with healthy donors. Based on the levels of piR-5937 and piR-28876, it was possible to differentiate between cancer patients and healthy donors with high sensitivity and specificity. Moreover, both piRNAs exhibited satisfactory diagnostic performance also in patients with stage I disease and enabled detection of colon cancer with higher sensitivity than currently used biomarkers CEA and CA19-9. Finally, the expression of analyzed piRNAs in blood restored significantly 1 month after the surgical resection. Conclusions: Based on our findings, piRNAs are abundant in human blood serum. Furthermore, their levels in colon cancer have been observed to be significantly deregulated. However, their involvement in carcinogenesis must be further established. Impact: piRNAs could serve as promising noninvasive biomarkers for early detection of colon cancer. (C) 2018 AACR.
ER -
VYCHYTILOVÁ, Petra, Karolína ŠTÍTKOVCOVÁ, Lenka RADOVÁ, Milana ŠACHLOVÁ, Zdeňka HERUDKOVÁ, Kateřina SLABÁ, Zdeněk KALA, Marek SVOBODA, Igor KISS, Rostislav VYZULA, William C. CHO a Ondřej SLABÝ. Circulating PIWI-Interacting RNAs piR-5937 and piR-28876 Are Promising Diagnostic Biomarkers of Colon Cancer. \textit{CANCER EPIDEMIOLOGY BIOMARKERS \&{}amp; PREVENTION}. PHILADELPHIA: AMER ASSOC CANCER RESEARCH, 2018, roč.~27, č.~9, s.~1019-1028. ISSN~1055-9965. Dostupné z: https://dx.doi.org/10.1158/1055-9965.EPI-18-0318.
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