Determination of testosterone and corticosterone in feathers
using liquid chromatography-mass spectrometry

J 2019

Determination of testosterone and corticosterone in feathers using liquid chromatography-mass spectrometry

BÍLKOVÁ, Zuzana; Marie KOTASOVÁ ADÁMKOVÁ; Tomáš ALBRECHT a Zdeněk ŠIMEK

Základní údaje

Originální název

Determination of testosterone and corticosterone in feathers using liquid chromatography-mass spectrometry

Autoři

BÍLKOVÁ, Zuzana; Marie KOTASOVÁ ADÁMKOVÁ; Tomáš ALBRECHT a Zdeněk ŠIMEK

Vydání

Journal of Chromatography A, Amsterdam, Netherlands, Elsevier, 2019, 0021-9673

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 4.049

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/19:00107240

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Feather; Hormones; Birds; Liquid chromatography; Tandem mass spectrometry; Chemical derivatization of ketosteroids

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 2. 2023 20:43, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Feathers gradually accumulate hormones and reflect long-term average plasma steroid levels during their growth. Feather hormone levels thus provide for the measurement of plasma hormones concentrations integrated over a period of several days or weeks. In this study, we focused on the development of a method to determine testosterone (TEST) and corticosterone (CORT) levels in extracts from feathers of small bodied birds with a limited amount of feathers available per individual. For this purpose, the method had to be verified for a small weight of samples. The present study describes the effect of the conditions of sample preparation and keto-derivatisation on the sensitivity of the LC-ESI-MS/MS analysis of TEST and CORT. Generally, chemical derivatization improves the sensitivity and selectivity of LC-MS/MS analysis. It can be used particularly in situations when the total amount of collected sample is limited (such as in our studies). Both the conditions of feather sample preparation (the selection of the extraction solvent, the time of extraction, and the conditions of solid phase extraction) and the reaction conditions affecting the formation of keto-derivatives (such as reaction temperature and reaction time) were tested. Methanol as the extraction solvent, 8 h as the extraction time, 50 degrees C as the reaction temperature of derivatization, and 90 min as the reaction time of derivatization are the most suitable conditions in terms of achieving a high sensitivity of analyses. Calibration curves are linear, at least in the range 25-2500 pg mL(-1), which is usually found in feather extracts. The limit of detection (LOD) for TEST and CORT was 1.0 and 0.3 pg per mL, respectively. The limit of quantification (LOQ) for TEST and CORT was 3.3 and 1.0 pg per mL, respectively. The optimized procedure was successfully applied for the analysis of TEST and CORT in real feather samples. The method could be used in a variety of research direction including wildlife, agricultural or veterinary studies.

Návaznosti

EF16_013/0001761, projekt VaV

Název: RECETOX RI

GA17-24782S, projekt VaV

Název: Latitudinální a altitudinální trendy v pace-of-life syndromech Afrotropických a Evropských pěvců

Investor: Grantová agentura ČR, Latitudinální a altitudinální trendy v pace-of-life syndromech Afrotropických a Evropských ptáků

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

Citovat

BÍLKOVÁ, Zuzana; Marie KOTASOVÁ ADÁMKOVÁ; Tomáš ALBRECHT a Zdeněk ŠIMEK. Determination of testosterone and corticosterone in feathers using liquid chromatography-mass spectrometry. Journal of Chromatography A. Amsterdam, Netherlands: Elsevier, 2019, roč. 1590, č. 1590, s. 96-103. ISSN 0021-9673. Dostupné z: https://doi.org/10.1016/j.chroma.2018.12.069.

@article{1490096,
   author = {Bílková, Zuzana and Kotasová Adámková, Marie and Albrecht, Tomáš and Šimek, Zdeněk},
   article_location = {Amsterdam, Netherlands},
   article_number = {1590},
   doi = {https://doi.org/10.1016/j.chroma.2018.12.069},
   keywords = {Feather; Hormones; Birds; Liquid chromatography; Tandem mass spectrometry; Chemical derivatization of ketosteroids},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Determination of testosterone and corticosterone in feathers using liquid chromatography-mass spectrometry},
   url = {https://www.sciencedirect.com/science/article/pii/S0021967318315693?via%3Dihub},
   volume = {1590},
   year = {2019}
}

TY  - JOUR
ID  - 1490096
AU  - Bílková, Zuzana - Kotasová Adámková, Marie - Albrecht, Tomáš - Šimek, Zdeněk
PY  - 2019
TI  - Determination of testosterone and corticosterone in feathers using liquid chromatography-mass spectrometry
JF  - Journal of Chromatography A
VL  - 1590
IS  - 1590
SP  - 96-103
EP  - 96-103
PB  - Elsevier
SN  - 00219673
KW  - Feather
KW  - Hormones
KW  - Birds
KW  - Liquid chromatography
KW  - Tandem mass spectrometry
KW  - Chemical derivatization of ketosteroids
UR  - https://www.sciencedirect.com/science/article/pii/S0021967318315693?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0021967318315693?via%3Dihub
N2  - Feathers gradually accumulate hormones and reflect long-term average plasma steroid levels during their growth. Feather hormone levels thus provide for the measurement of plasma hormones concentrations integrated over a period of several days or weeks. In this study, we focused on the development of a method to determine testosterone (TEST) and corticosterone (CORT) levels in extracts from feathers of small bodied birds with a limited amount of feathers available per individual. For this purpose, the method had to be verified for a small weight of samples. The present study describes the effect of the conditions of sample preparation and keto-derivatisation on the sensitivity of the LC-ESI-MS/MS analysis of TEST and CORT. Generally, chemical derivatization improves the sensitivity and selectivity of LC-MS/MS analysis. It can be used particularly in situations when the total amount of collected sample is limited (such as in our studies). Both the conditions of feather sample preparation (the selection of the extraction solvent, the time of extraction, and the conditions of solid phase extraction) and the reaction conditions affecting the formation of keto-derivatives (such as reaction temperature and reaction time) were tested. Methanol as the extraction solvent, 8 h as the extraction time, 50 degrees C as the reaction temperature of derivatization, and 90 min as the reaction time of derivatization are the most suitable conditions in terms of achieving a high sensitivity of analyses. Calibration curves are linear, at least in the range 25-2500 pg mL(-1), which is usually found in feather extracts. The limit of detection (LOD) for TEST and CORT was 1.0 and 0.3 pg per mL, respectively. The limit of quantification (LOQ) for TEST and CORT was 3.3 and 1.0 pg per mL, respectively. The optimized procedure was successfully applied for the analysis of TEST and CORT in real feather samples. The method could be used in a variety of research direction including wildlife, agricultural or veterinary studies.
ER  -

BÍLKOVÁ, Zuzana; Marie KOTASOVÁ ADÁMKOVÁ; Tomáš ALBRECHT a Zdeněk ŠIMEK. Determination of testosterone and corticosterone in feathers using liquid chromatography-mass spectrometry. \textit{Journal of Chromatography A}. Amsterdam, Netherlands: Elsevier, 2019, roč.~1590, č.~1590, s.~96-103. ISSN~0021-9673. Dostupné z: https://doi.org/10.1016/j.chroma.2018.12.069.

Přehled o publikaci