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@article{1490403,
author = {Bartošík, Martin and Jiráková, Ludmila and Anton, Milan and Vojtěšek, Bořivoj and Hrstka, Roman},
article_location = {Amsterdam},
article_number = {DEC 26 2018},
doi = {http://dx.doi.org/10.1016/j.aca.2018.08.020},
keywords = {HPV; Cervical cancer; Genomagnetic assay; Electrode chip; LAMP amplification},
language = {eng},
issn = {0003-2670},
journal = {Analytica Chimica Acta},
title = {Genomagnetic LAMP-based electrochemical test for determination of high-risk HPV16 and HPV18 in clinical samples},
url = {https://www.sciencedirect.com/science/article/pii/S0003267018309723},
volume = {1042},
year = {2018}
}
TY - JOUR
ID - 1490403
AU - Bartošík, Martin - Jiráková, Ludmila - Anton, Milan - Vojtěšek, Bořivoj - Hrstka, Roman
PY - 2018
TI - Genomagnetic LAMP-based electrochemical test for determination of high-risk HPV16 and HPV18 in clinical samples
JF - Analytica Chimica Acta
VL - 1042
IS - DEC 26 2018
SP - 37-43
EP - 37-43
PB - Elsevier Science publishers
SN - 00032670
KW - HPV
KW - Cervical cancer
KW - Genomagnetic assay
KW - Electrode chip
KW - LAMP amplification
UR - https://www.sciencedirect.com/science/article/pii/S0003267018309723
N2 - Major cause of cervical cancer is a persistent infection with high-risk types of human papillomaviruses (HPV). For that reason, HPV testing is now becoming an important addition to standard cytological screening of cervical malignancies in women (known as Pap test). New methods are sought which could offer rapid and inexpensive detection schemes, such as those based on electrochemical (EC) readout. Here, we developed an assay for parallel detection of two most oncogenic high-risk HPV types, HPV16 and HPV18, by combining loop-mediated amplification (LAMP) of template DNA, its separation using magnetic beads and detection with amperometry at carbon-based electrode chips. Our EC-LAMP test enabled us to successfully discriminate both HPV types not only in cancer cell lines, but also using clinical material obtained from HPV-positive patient samples. (C 2018 Elsevier B.V. All rights reserved.
ER -
BARTOŠÍK, Martin, Ludmila JIRÁKOVÁ, Milan ANTON, Bořivoj VOJTĚŠEK a Roman HRSTKA. Genomagnetic LAMP-based electrochemical test for determination of high-risk HPV16 and HPV18 in clinical samples. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier Science publishers, 2018, roč.~1042, DEC 26 2018, s.~37-43. ISSN~0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2018.08.020.
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