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@article{1491959,
author = {Hrubanova, Kamila and Krzyzanek, Vladislav and Nebesarova, Jana and Růžička, Filip and Pilat, Zdenek and Samek, Ota},
article_location = {Basel},
article_number = {12},
doi = {http://dx.doi.org/10.3390/s18124089},
keywords = {Raman spectroscopy; biofilm; sample preparation; scanning electron microscopy; cryo-SEM},
language = {eng},
issn = {1424-8220},
journal = {Sensors},
title = {Monitoring Candida parapsilosis and Staphylococcus epidermidis Biofilms by a Combination of Scanning Electron Microscopy and Raman Spectroscopy},
volume = {18},
year = {2018}
}
TY - JOUR
ID - 1491959
AU - Hrubanova, Kamila - Krzyzanek, Vladislav - Nebesarova, Jana - Růžička, Filip - Pilat, Zdenek - Samek, Ota
PY - 2018
TI - Monitoring Candida parapsilosis and Staphylococcus epidermidis Biofilms by a Combination of Scanning Electron Microscopy and Raman Spectroscopy
JF - Sensors
VL - 18
IS - 12
SP - 1-19
EP - 1-19
PB - MDPI AG
SN - 14248220
KW - Raman spectroscopy
KW - biofilm
KW - sample preparation
KW - scanning electron microscopy
KW - cryo-SEM
N2 - The biofilm-forming microbial species Candida parapsilosis and Staphylococcus epidermidis have been recently linked to serious infections associated with implanted medical devices. We studied microbial biofilms by high resolution scanning electron microscopy (SEM), which allowed us to visualize the biofilm structure, including the distribution of cells inside the extracellular matrix and the areas of surface adhesion. We compared classical SEM (chemically fixed samples) with cryogenic SEM, which employs physical sample preparation based on plunging the sample into various liquid cryogens, as well as high-pressure freezing (HPF). For imaging the biofilm interior, we applied the freeze-fracture technique. In this study, we show that the different means of sample preparation have a fundamental influence on the observed biofilm structure. We complemented the SEM observations with Raman spectroscopic analysis, which allowed us to assess the time-dependent chemical composition changes of the biofilm in vivo. We identified the individual spectral peaks of the biomolecules present in the biofilm and we employed principal component analysis (PCA) to follow the temporal development of the chemical composition.
ER -
HRUBANOVA, Kamila, Vladislav KRZYZANEK, Jana NEBESAROVA, Filip RŮŽIČKA, Zdenek PILAT a Ota SAMEK. Monitoring Candida parapsilosis and Staphylococcus epidermidis Biofilms by a Combination of Scanning Electron Microscopy and Raman Spectroscopy. \textit{Sensors}. Basel: MDPI AG, roč.~18, č.~12, s.~1-19. ISSN~1424-8220. doi:10.3390/s18124089. 2018.
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