When is a “cryptic” species not a cryptic species: A
consideration from the Holarctic micro-landsnail genus
Euconulus (Gastropoda: Stylommatophora)

J 2019

When is a “cryptic” species not a cryptic species: A consideration from the Holarctic micro-landsnail genus Euconulus (Gastropoda: Stylommatophora)

HORSÁKOVÁ, Veronika; Jeffrey Clark NEKOLA a Michal HORSÁK

Základní údaje

Originální název

When is a “cryptic” species not a cryptic species: A consideration from the Holarctic micro-landsnail genus Euconulus (Gastropoda: Stylommatophora)

Autoři

HORSÁKOVÁ, Veronika; Jeffrey Clark NEKOLA a Michal HORSÁK

Vydání

Molecular Phylogenetics and Evolution, Výsledky hledání Výsledky vyhledávání na, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2019, 1055-7903

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10602 Biology , Evolutionary biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 3.496

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/19:00107259

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Cryptic species; Euconulus; Holarctic range; Micro-land snails; Phylogeny; Shell morphology

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 3. 2020 13:25, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Naive use of molecular data may lead to ambiguous conclusions, especially within the context of “cryptic” species. Here, we integrated molecular and morphometric data to evaluate phylogenetic relationships in the widespread terrestrial micro-snail genus, Euconulus. We analyzed mitochondrial (16S + COII) and nuclear (ITS1 + ITS2) sequence across 94 populations from Europe, Asia and North America within the nominate species E. alderi, E. fulvus and E. polygyratus, and used the southeastern USA E. chersinus, E. dentatus, and E. trochulus as comparative outgroups. Phylogeny was reconstructed using four different reconstruction methods to identify robust, well-supported topological features. We then performed discriminant analysis on shell measurements between these genetically-identified species-level clades. These analyses provided evidence for a biologically valid North American “cryptic” species within E. alderi. However, while highly supported polyphyletic structure was also observed within E. fulvus, disagreement in placement of individuals between mtDNA and nDNA clades, lack of morphological differences, and presence of potential hybrids imply that these lineages do not rise to the threshold as biologically valid cryptic species, and rather appear to simply represent a complex of geographically structured populations within a single species. These results caution that entering into a cryptic species hypothesis should not be undertaken lightly, and should be optimally supported along multiple lines of evidence. Generally, post-hoc analyses of macro-scale features should be conducted to attempt identification of previously ignored diagnostic traits. If such traits cannot be found, i.e. in the case of potentially “fully cryptic” species, additional criteria should be met to propound a cryptic species hypothesis, including the agreement in tree topology among both mtDNA and nDNA, and little (or no) evidence of hybridization based on a critical analysis of sequence chromatograms. Even when the above conditions are satisfied, it only implies that the cryptic species hypothesis is plausible, but should optimally be subjected to further careful examination.

Návaznosti

GA17-05696S, projekt VaV

Název: Holocenní vývoj evropské bioty mírného pásu: vlivy klimatu, refugií a lokálních faktorů testované na komplexních datech nezávislých proxy

Investor: Grantová agentura ČR, Holocenní vývoj evropské bioty mírného pásu: vlivy klimatu, refugií a lokálních faktorů testované na komplexních datech nezávislých proxy

Citovat

HORSÁKOVÁ, Veronika; Jeffrey Clark NEKOLA a Michal HORSÁK. When is a “cryptic” species not a cryptic species: A consideration from the Holarctic micro-landsnail genus Euconulus (Gastropoda: Stylommatophora). Molecular Phylogenetics and Evolution. Výsledky hledání Výsledky vyhledávání na: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2019, roč. 132, MAR 2019, s. 307-320. ISSN 1055-7903. Dostupné z: https://doi.org/10.1016/j.ympev.2018.12.004.

@article{1495967,
   author = {Horsáková, Veronika and Nekola, Jeffrey Clark and Horsák, Michal},
   article_location = {Výsledky hledání Výsledky vyhledávání na},
   article_number = {MAR 2019},
   doi = {https://doi.org/10.1016/j.ympev.2018.12.004},
   keywords = {Cryptic species; Euconulus; Holarctic range; Micro-land snails; Phylogeny; Shell morphology},
   language = {eng},
   issn = {1055-7903},
   journal = {Molecular Phylogenetics and Evolution},
   title = {When is a “cryptic” species not a cryptic species: A consideration from the Holarctic micro-landsnail genus Euconulus (Gastropoda: Stylommatophora)},
   url = {https://www.sciencedirect.com/science/article/pii/S105579031830215X},
   volume = {132},
   year = {2019}
}

TY  - JOUR
ID  - 1495967
AU  - Horsáková, Veronika - Nekola, Jeffrey Clark - Horsák, Michal
PY  - 2019
TI  - When is a “cryptic” species not a cryptic species: A consideration from the Holarctic micro-landsnail genus Euconulus (Gastropoda: Stylommatophora)
JF  - Molecular Phylogenetics and Evolution
VL  - 132
IS  - MAR 2019
SP  - 307-320
EP  - 307-320
PB  - ACADEMIC PRESS INC ELSEVIER SCIENCE
SN  - 10557903
KW  - Cryptic species
KW  - Euconulus
KW  - Holarctic range
KW  - Micro-land snails
KW  - Phylogeny
KW  - Shell morphology
UR  - https://www.sciencedirect.com/science/article/pii/S105579031830215X
L2  - https://www.sciencedirect.com/science/article/pii/S105579031830215X
N2  - Naive use of molecular data may lead to ambiguous conclusions, especially within the context of “cryptic” species. Here, we integrated molecular and morphometric data to evaluate phylogenetic relationships in the widespread terrestrial micro-snail genus, Euconulus. We analyzed mitochondrial (16S + COII) and nuclear (ITS1 + ITS2) sequence across 94 populations from Europe, Asia and North America within the nominate species E. alderi, E. fulvus and E. polygyratus, and used the southeastern USA E. chersinus, E. dentatus, and E. trochulus as comparative outgroups. Phylogeny was reconstructed using four different reconstruction methods to identify robust, well-supported topological features. We then performed discriminant analysis on shell measurements between these genetically-identified species-level clades. These analyses provided evidence for a biologically valid North American “cryptic” species within E. alderi. However, while highly supported polyphyletic structure was also observed within E. fulvus, disagreement in placement of individuals between mtDNA and nDNA clades, lack of morphological differences, and presence of potential hybrids imply that these lineages do not rise to the threshold as biologically valid cryptic species, and rather appear to simply represent a complex of geographically structured populations within a single species. These results caution that entering into a cryptic species hypothesis should not be undertaken lightly, and should be optimally supported along multiple lines of evidence. Generally, post-hoc analyses of macro-scale features should be conducted to attempt identification of previously ignored diagnostic traits. If such traits cannot be found, i.e. in the case of potentially “fully cryptic” species, additional criteria should be met to propound a cryptic species hypothesis, including the agreement in tree topology among both mtDNA and nDNA, and little (or no) evidence of hybridization based on a critical analysis of sequence chromatograms. Even when the above conditions are satisfied, it only implies that the cryptic species hypothesis is plausible, but should optimally be subjected to further careful examination.
ER  -

HORSÁKOVÁ, Veronika; Jeffrey Clark NEKOLA a Michal HORSÁK. When is a “cryptic” species not a cryptic species: A consideration from the Holarctic micro-landsnail genus Euconulus (Gastropoda: Stylommatophora). \textit{Molecular Phylogenetics and Evolution}. Výsledky hledání Výsledky vyhledávání na: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2019, roč.~132, MAR 2019, s.~307-320. ISSN~1055-7903. Dostupné z: https://doi.org/10.1016/j.ympev.2018.12.004.

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