Advancement of the cultivation and upscaling of
photoautotrophic suspension cultures using Chenopodium rubrum
as a case study

J 2018

Advancement of the cultivation and upscaling of photoautotrophic suspension cultures using Chenopodium rubrum as a case study

SEGEČOVÁ, Anna; Jan ČERVENÝ a Thomas ROITSCH

Základní údaje

Originální název

Advancement of the cultivation and upscaling of photoautotrophic suspension cultures using Chenopodium rubrum as a case study

Autoři

SEGEČOVÁ, Anna; Jan ČERVENÝ a Thomas ROITSCH

Vydání

Plant Cell, Tissue and Organ Culture, Dordrecht, Springer, 2018, 0167-6857

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

20802 Bioremediation, diagnostic biotechnologies in environmental management

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.200

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/18:00109166

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Biotechnology; Growth characterization; Photobioreactor; Photosynthesis; Physiology

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 5. 2020 12:30, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Photoautotrophic (PA) suspension cultures combine the advantages of cell suspensions with carbon autotrophy and have been used for various applications including studies on photosynthesis, sugar signal transduction, herbicide action, and stress responses. A major practical drawback is the requirement for elevated CO2, which is typically generated by a buffer system in two-tier flasks that need to be custom made and require time-consuming handling. In this study, we substantially simplified and improved the cultivation of a PA culture of Chenopodium rubrum as a case study in Erlenmeyer flasks shaken in a CO2 enriched photoincubator. Growth rates and photosynthetic activity were found to be comparable to those in two-tier flasks but with the advantage of constant CO2 level throughout the cultivation. In addition, it was possible to establish the cultivation in a commercial laboratory-scale photobioreactor with various options to control and continuously measure the culture conditions and regimes and assess the physiological status. Although the relative biomass yield and photosynthetic performance of the batch cultures was not fully reached, the continuous cultivation system provides a good basis for future scale-up if larger amounts of biomass are needed or to impose specific cultivation regimes in combination with comprehensive online monitoring. The different improvements should contribute to the more widespread use of higher plant PA cultures and also facilitate the cultivation of microalgae.

Návaznosti

LM2015055, projekt VaV

Název: Centrum pro systémovou biologii (Akronym: C4SYS)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, The national infrastructure C4SYS - Centre for Systems Biology

Citovat

SEGEČOVÁ, Anna; Jan ČERVENÝ a Thomas ROITSCH. Advancement of the cultivation and upscaling of photoautotrophic suspension cultures using Chenopodium rubrum as a case study. Plant Cell, Tissue and Organ Culture. Dordrecht: Springer, 2018, roč. 135, č. 1, s. 37-51. ISSN 0167-6857. Dostupné z: https://doi.org/10.1007/s11240-018-1441-6.

@article{1498716,
   author = {Segečová, Anna and Červený, Jan and Roitsch, Thomas},
   article_location = {Dordrecht},
   article_number = {1},
   doi = {https://doi.org/10.1007/s11240-018-1441-6},
   keywords = {Biotechnology; Growth characterization; Photobioreactor; Photosynthesis; Physiology},
   language = {eng},
   issn = {0167-6857},
   journal = {Plant Cell, Tissue and Organ Culture},
   title = {Advancement of the cultivation and upscaling of photoautotrophic suspension cultures using Chenopodium rubrum as a case study},
   url = {https://link.springer.com/article/10.1007/s11240-018-1441-6},
   volume = {135},
   year = {2018}
}

TY  - JOUR
ID  - 1498716
AU  - Segečová, Anna - Červený, Jan - Roitsch, Thomas
PY  - 2018
TI  - Advancement of the cultivation and upscaling of photoautotrophic suspension cultures using Chenopodium rubrum as a case study
JF  - Plant Cell, Tissue and Organ Culture
VL  - 135
IS  - 1
SP  - 37-51
EP  - 37-51
PB  - Springer
SN  - 01676857
KW  - Biotechnology
KW  - Growth characterization
KW  - Photobioreactor
KW  - Photosynthesis
KW  - Physiology
UR  - https://link.springer.com/article/10.1007/s11240-018-1441-6
L2  - https://link.springer.com/article/10.1007/s11240-018-1441-6
N2  - Photoautotrophic (PA) suspension cultures combine the advantages of cell suspensions with carbon autotrophy and have been used for various applications including studies on photosynthesis, sugar signal transduction, herbicide action, and stress responses. A major practical drawback is the requirement for elevated CO2, which is typically generated by a buffer system in two-tier flasks that need to be custom made and require time-consuming handling. In this study, we substantially simplified and improved the cultivation of a PA culture of Chenopodium rubrum as a case study in Erlenmeyer flasks shaken in a CO2 enriched photoincubator. Growth rates and photosynthetic activity were found to be comparable to those in two-tier flasks but with the advantage of constant CO2 level throughout the cultivation. In addition, it was possible to establish the cultivation in a commercial laboratory-scale photobioreactor with various options to control and continuously measure the culture conditions and regimes and assess the physiological status. Although the relative biomass yield and photosynthetic performance of the batch cultures was not fully reached, the continuous cultivation system provides a good basis for future scale-up if larger amounts of biomass are needed or to impose specific cultivation regimes in combination with comprehensive online monitoring. The different improvements should contribute to the more widespread use of higher plant PA cultures and also facilitate the cultivation of microalgae.
ER  -

SEGEČOVÁ, Anna; Jan ČERVENÝ a Thomas ROITSCH. Advancement of the cultivation and upscaling of photoautotrophic suspension cultures using Chenopodium rubrum as a case study. \textit{Plant Cell, Tissue and Organ Culture}. Dordrecht: Springer, 2018, roč.~135, č.~1, s.~37-51. ISSN~0167-6857. Dostupné z: https://doi.org/10.1007/s11240-018-1441-6.

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