A novel perspective on MOL-PCR optimization and MAGPIX analysis
of in-house multiplex foodborne pathogens detection assay

J 2019

A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay

RESLOVÁ, Nikol; Veronika HUVAROVÁ; Jakub HRDÝ; Martin KAŠNÝ; Petr KRÁLÍK et al.

Základní údaje

Originální název

A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay

Autoři

RESLOVÁ, Nikol; Veronika HUVAROVÁ; Jakub HRDÝ; Martin KAŠNÝ a Petr KRÁLÍK

Vydání

Scientific reports, London, Nature Publishing Group, 2019, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 3.998

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/19:00109199

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

xMAP; multiplexní detekce; potravinové patogeny; optimalizace; MAGPIX

Klíčová slova anglicky

xMAP; multiplex detection; foodborne pathogens; optimization; MAGPIX

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 3. 2020 16:04, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Multiplex oligonucleotide ligation-PCR (MOL-PCR) is a rapid method for simultaneous detection of multiple molecular markers within a single reaction. MOL-PCR is increasingly employed in microbial detection assays, where its ability to facilitate identification and further characterization via simple analysis is of great benefit and significantly simplifies routine diagnostics. When adapted to microsphere suspension arrays on a MAGPIX reader, MOL-PCR has the potential to outperform standard nucleic acid-based diagnostic assays. This study represents the guideline towards in-house MOL-PCR assay optimization using the example of foodborne pathogens (bacteria and parasites) with an emphasis on the appropriate choice of crucial parameters. The optimized protocol focused on specific sequence detection utilizes the fluorescent reporter BODIPY-TMRX and self-coupled magnetic microspheres and allows for a smooth and brisk workflow which should serve as a guide for the development of MOL-PCR assays intended for pathogen detection.

Návaznosti

MUNI/A/0816/2017, interní kód MU

Název: Výzkum ekologicko-evolučních vztahů bezobratlých živočichů (Akronym: EKOLINKS)

Investor: Masarykova univerzita, Výzkum ekologicko-evolučních vztahů bezobratlých živočichů, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Přiložené soubory

Reslova_et_al_2019_MOL-PCR_xMAP.pdf Verze souboru

Citovat

RESLOVÁ, Nikol; Veronika HUVAROVÁ; Jakub HRDÝ; Martin KAŠNÝ a Petr KRÁLÍK. A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay. Scientific reports. London: Nature Publishing Group, 2019, roč. 9, č. 2719, s. 1-13. ISSN 2045-2322. Dostupné z: https://doi.org/10.1038/s41598-019-40035-5.

@article{1501505,
   author = {Reslová, Nikol and Huvarová, Veronika and Hrdý, Jakub and Kašný, Martin and Králík, Petr},
   article_location = {London},
   article_number = {2719},
   doi = {https://doi.org/10.1038/s41598-019-40035-5},
   keywords = {xMAP; multiplex detection; foodborne pathogens; optimization; MAGPIX},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific reports},
   title = {A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay},
   url = {https://rdcu.be/boqEh},
   volume = {9},
   year = {2019}
}

TY  - JOUR
ID  - 1501505
AU  - Reslová, Nikol - Huvarová, Veronika - Hrdý, Jakub - Kašný, Martin - Králík, Petr
PY  - 2019
TI  - A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
JF  - Scientific reports
VL  - 9
IS  - 2719
SP  - 1-13
EP  - 1-13
PB  - Nature Publishing Group
SN  - 20452322
KW  - xMAP
KW  - multiplex detection
KW  - foodborne pathogens
KW  - optimization
KW  - MAGPIX
UR  - https://rdcu.be/boqEh
L2  - https://rdcu.be/boqEh
N2  - Multiplex oligonucleotide ligation-PCR (MOL-PCR) is a rapid method for simultaneous detection of multiple molecular markers within a single reaction. MOL-PCR is increasingly employed in microbial detection assays, where its ability to facilitate identification and further characterization via simple analysis is of great benefit and significantly simplifies routine diagnostics. When adapted to microsphere suspension arrays on a MAGPIX reader, MOL-PCR has the potential to outperform standard nucleic acid-based diagnostic assays. This study represents the guideline towards in-house MOL-PCR assay optimization using the example of foodborne pathogens (bacteria and parasites) with an emphasis on the appropriate choice of crucial parameters. The optimized protocol focused on specific sequence detection utilizes the fluorescent reporter BODIPY-TMRX and self-coupled magnetic microspheres and allows for a smooth and brisk workflow which should serve as a guide for the development of MOL-PCR assays intended for pathogen detection.
ER  -

RESLOVÁ, Nikol; Veronika HUVAROVÁ; Jakub HRDÝ; Martin KAŠNÝ a Petr KRÁLÍK. A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay. \textit{Scientific reports}. London: Nature Publishing Group, 2019, roč.~9, č.~2719, s.~1-13. ISSN~2045-2322. Dostupné z: https://doi.org/10.1038/s41598-019-40035-5.

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