Study of lectins from opportunistic pathogens and their
interaction with normal human cells.

a 2019

Study of lectins from opportunistic pathogens and their interaction with normal human cells.

NOTOVÁ, Simona; Veronika SEDLÁKOVÁ; Michaela KLOUČKOVÁ; Marek KORSÁK; Martin KOLLÁR et al.

Základní údaje

Originální název

Study of lectins from opportunistic pathogens and their interaction with normal human cells.

Autoři

NOTOVÁ, Simona; Veronika SEDLÁKOVÁ; Michaela KLOUČKOVÁ; Marek KORSÁK; Martin KOLLÁR; Aleš HAMPL a Michaela WIMMEROVÁ

Vydání

GlycoBioTec2019, 2019

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/19:00107308

Organizační jednotka

Středoevropský technologický institut

Klíčová slova česky

lektiny; lidské buňky; fluorescenční mikroskopie

Klíčová slova anglicky

lectins; human cells; fluorescence microscopy

Štítky

rivok

Příznaky

Mezinárodní význam

Změněno: 11. 3. 2020 18:09, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Lectins are carbohydrate-binding proteins, highly specific for sugar moieties. They can be involved in the attachment of bacteria and viruses on their host epithelial surfaces, which is one of the most critical step leading to infection. Since the antibiotic strategy is not fully efficient anymore, new ways to cure bacterial infections are under development. One of the possibilities is anti-adhesion/anti-lectin therapy where the lectin can be inhibited by its ligand. For bringing this therapy to the medicine, first we need to identify a binding partner of the lectin and then to provide inhibition tests which approve specificity of lectins. In this work we studied interactions between lectins from Burkholderia cenocepacia and Photorhabdus luminescens, with several different types of normal non-cancerous human cells. Both lectins are fucose specific and calcium independent. The interaction was detected by fluorescence microscopy and for each observed interaction we provided inhibition tests. The lectin from B. cenocepacia interacted with surface glycoproteins of embryonal stem cells and early lung epithelial progenitors but did not bind any ligand on the surface of neural stem cells. In contrary, the lectin from P. luminescens interacted only with mesenchymal stromal cells. The obtained results may bring new promising possibilities to the research and medicine.

Návaznosti

GA18-18964S, projekt VaV

Název: Lektiny a jejich úloha v interakci patogen/hostitel a buněčném rozpoznávání

Investor: Grantová agentura ČR, Lektiny a jejich úloha v interakci patogen/hostitel a buněčném rozpoznávání

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

NOTOVÁ, Simona; Veronika SEDLÁKOVÁ; Michaela KLOUČKOVÁ; Marek KORSÁK; Martin KOLLÁR; Aleš HAMPL a Michaela WIMMEROVÁ. Study of lectins from opportunistic pathogens and their interaction with normal human cells. In GlycoBioTec2019. 2019.

@proceedings{1504277,
   author = {Notová, Simona and Sedláková, Veronika and Kloučková, Michaela and Korsák, Marek and Kollár, Martin and Hampl, Aleš and Wimmerová, Michaela},
   booktitle = {GlycoBioTec2019},
   keywords = {lectins; human cells; fluorescence microscopy},
   language = {eng},
   title = {Study of lectins from opportunistic pathogens and their interaction with normal human cells.},
   year = {2019}
}

TY  - CONF
ID  - 1504277
AU  - Notová, Simona - Sedláková, Veronika - Kloučková, Michaela - Korsák, Marek - Kollár, Martin - Hampl, Aleš - Wimmerová, Michaela
PY  - 2019
TI  - Study of lectins from opportunistic pathogens and their interaction with normal human cells.
KW  - lectins
KW  - human cells
KW  - fluorescence microscopy
N2  - Lectins are carbohydrate-binding proteins, highly specific for sugar moieties. They can be involved in the attachment of bacteria and viruses on their host epithelial surfaces, which is one of the most critical step leading to infection. Since the antibiotic strategy is not fully efficient anymore, new ways to cure bacterial infections are under development. One of the possibilities is anti-adhesion/anti-lectin therapy where the lectin can be inhibited by its ligand. For bringing this therapy to the medicine, first we need to identify a binding partner of the lectin and then to provide inhibition tests which approve specificity of lectins. In this work we studied interactions between lectins from Burkholderia cenocepacia and Photorhabdus luminescens, with several different types of normal non-cancerous human cells. Both lectins are fucose specific and calcium independent. The interaction was detected by fluorescence microscopy and for each observed interaction we provided inhibition tests. The lectin from B. cenocepacia interacted with surface glycoproteins of embryonal stem cells and early lung epithelial progenitors but did not bind any ligand on the surface of neural stem cells. In contrary, the lectin from P. luminescens interacted only with mesenchymal stromal cells. The obtained results may bring new promising possibilities to the research and medicine.
ER  -

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