Plasticity and intratumoural heterogeneity of cell surface
antigen expression in breast cancer

J 2018

Plasticity and intratumoural heterogeneity of cell surface antigen expression in breast cancer

REMŠÍK, Ján; Radek FEDR; J. NAVRATIL; Lucia BINÓ; Eva SLABÁKOVÁ et al.

Základní údaje

Originální název

Plasticity and intratumoural heterogeneity of cell surface antigen expression in breast cancer

Autoři

REMŠÍK, Ján; Radek FEDR; J. NAVRATIL; Lucia BINÓ; Eva SLABÁKOVÁ; P. FABIAN; M. SVOBODA a Karel SOUČEK

Vydání

British journal of cancer, London, Nature Publishing Group, 2018, 0007-0920

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.416

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/18:00106565

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

breast cancer; epithelial-mesenchymal plasticity; intratumoural heterogeneity; CD9

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 4. 2024 14:27, Mgr. Michal Petr

Anotace

V originále

Background: The intratumoural heterogeneity, often driven by epithelial-to-mesenchymal transition (EMT), significantly contributes to chemoresistance and disease progression in adenocarcinomas. Methods: We introduced a high-throughput screening platform to identify surface antigens that associate with epithelial-mesenchymal plasticity in well-defined pairs of epithelial cell lines and their mesenchymal counterparts. Using multicolour flow cytometry, we then analysed the expression of 10 most robustly changed antigens and identified a 10-molecule surface signature, in pan-cytokeratin-positive/EpCAM-positive and -negative fractions of dissociated breast tumours. Results: We found that surface CD9, CD29, CD49c, and integrin beta 5 are lost in breast cancer cells that underwent EMT in vivo. The tetraspanin family member CD9 was concordantly downregulated both in vitro and in vivo and associated with epithelial phenotype and favourable prognosis. Conclusions: We propose that overall landscape of 10-molecule surface signature expression reflects the epithelial-mesenchymal plasticity in breast cancer.

Citovat

REMŠÍK, Ján; Radek FEDR; J. NAVRATIL; Lucia BINÓ; Eva SLABÁKOVÁ; P. FABIAN; M. SVOBODA a Karel SOUČEK. Plasticity and intratumoural heterogeneity of cell surface antigen expression in breast cancer. British journal of cancer. London: Nature Publishing Group, 2018, roč. 118, č. 6, s. 813-819. ISSN 0007-0920. Dostupné z: https://doi.org/10.1038/bjc.2017.497.

@article{1504862,
   author = {Remšík, Ján and Fedr, Radek and Navratil, J. and Binó, Lucia and Slabáková, Eva and Fabian, P. and Svoboda, M. and Souček, Karel},
   article_location = {London},
   article_number = {6},
   doi = {https://doi.org/10.1038/bjc.2017.497},
   keywords = {breast cancer; epithelial-mesenchymal plasticity; intratumoural heterogeneity; CD9},
   language = {eng},
   issn = {0007-0920},
   journal = {British journal of cancer},
   title = {Plasticity and intratumoural heterogeneity of cell surface antigen expression in breast cancer},
   url = {http://dx.doi.org/10.1038/bjc.2017.497},
   volume = {118},
   year = {2018}
}

TY  - JOUR
ID  - 1504862
AU  - Remšík, Ján - Fedr, Radek - Navratil, J. - Binó, Lucia - Slabáková, Eva - Fabian, P. - Svoboda, M. - Souček, Karel
PY  - 2018
TI  - Plasticity and intratumoural heterogeneity of cell surface antigen expression in breast cancer
JF  - British journal of cancer
VL  - 118
IS  - 6
SP  - 813-819
EP  - 813-819
PB  - Nature Publishing Group
SN  - 00070920
KW  - breast cancer
KW  - epithelial-mesenchymal plasticity
KW  - intratumoural heterogeneity
KW  - CD9
UR  - http://dx.doi.org/10.1038/bjc.2017.497
N2  - Background: The intratumoural heterogeneity, often driven by epithelial-to-mesenchymal transition (EMT), significantly contributes to chemoresistance and disease progression in adenocarcinomas. Methods: We introduced a high-throughput screening platform to identify surface antigens that associate with epithelial-mesenchymal plasticity in well-defined pairs of epithelial cell lines and their mesenchymal counterparts. Using multicolour flow cytometry, we then analysed the expression of 10 most robustly changed antigens and identified a 10-molecule surface signature, in pan-cytokeratin-positive/EpCAM-positive and -negative fractions of dissociated breast tumours. Results: We found that surface CD9, CD29, CD49c, and integrin beta 5 are lost in breast cancer cells that underwent EMT in vivo. The tetraspanin family member CD9 was concordantly downregulated both in vitro and in vivo and associated with epithelial phenotype and favourable prognosis. Conclusions: We propose that overall landscape of 10-molecule surface signature expression reflects the epithelial-mesenchymal plasticity in breast cancer.
ER  -

REMŠÍK, Ján; Radek FEDR; J. NAVRATIL; Lucia BINÓ; Eva SLABÁKOVÁ; P. FABIAN; M. SVOBODA a Karel SOUČEK. Plasticity and intratumoural heterogeneity of cell surface antigen expression in breast cancer. \textit{British journal of cancer}. London: Nature Publishing Group, 2018, roč.~118, č.~6, s.~813-819. ISSN~0007-0920. Dostupné z: https://doi.org/10.1038/bjc.2017.497.

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