Conserved cysteines in Mason-Pfizer monkey virus capsid protein
are essential for infectious mature particle formation

J 2018

Conserved cysteines in Mason-Pfizer monkey virus capsid protein are essential for infectious mature particle formation

PICHALOVA, R.; Tibor FÜZIK; B. VOKATA; M. RUMLOVA; M. LLANO et al.

Základní údaje

Originální název

Conserved cysteines in Mason-Pfizer monkey virus capsid protein are essential for infectious mature particle formation

Autoři

PICHALOVA, R.; Tibor FÜZIK ; B. VOKATA; M. RUMLOVA; M. LLANO; A. DOSTALKOVA; I. KRIZOVA; T. RUML a P. ULBRICH

Vydání

Virology, SAN DIEGO, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2018, 0042-6822

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.657

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/18:00106575

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

M-PMV capsid; Cysteine mutagenesis; Retrovirus assembly; Virus core stability; M-PMV infectivity

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 3. 2019 11:13, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Retrovirus assembly is driven mostly by Gag polyprotein oligomerization, which is mediated by inter and intra protein protein interactions among its capsid (CA) domains. Mason-Pfizer monkey virus (M-PMV) CA contains three cysteines (C82, C193 and C213), where the latter two are highly conserved among most retroviruses. To determine the importance of these cysteines, we introduced mutations of these residues in both bacterial and proviral vectors and studied their impact on the M-PMV life cycle. These studies revealed that the presence of both conserved cysteines of M-PMV CA is necessary for both proper assembly and virus infectivity. Our findings suggest a crucial role of these cysteines in the formation of infectious mature particles.

Citovat

PICHALOVA, R.; Tibor FÜZIK; B. VOKATA; M. RUMLOVA; M. LLANO; A. DOSTALKOVA; I. KRIZOVA; T. RUML a P. ULBRICH. Conserved cysteines in Mason-Pfizer monkey virus capsid protein are essential for infectious mature particle formation. Virology. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2018, roč. 521, AUG, s. 108-117. ISSN 0042-6822. Dostupné z: https://doi.org/10.1016/j.virol.2018.06.001.

@article{1505088,
   author = {Pichalova, R. and Füzik, Tibor and Vokata, B. and Rumlova, M. and Llano, M. and Dostalkova, A. and Krizova, I. and Ruml, T. and Ulbrich, P.},
   article_location = {SAN DIEGO},
   article_number = {AUG},
   doi = {https://doi.org/10.1016/j.virol.2018.06.001},
   keywords = {M-PMV capsid; Cysteine mutagenesis; Retrovirus assembly; Virus core stability; M-PMV infectivity},
   language = {eng},
   issn = {0042-6822},
   journal = {Virology},
   title = {Conserved cysteines in Mason-Pfizer monkey virus capsid protein are essential for infectious mature particle formation},
   volume = {521},
   year = {2018}
}

TY  - JOUR
ID  - 1505088
AU  - Pichalova, R. - Füzik, Tibor - Vokata, B. - Rumlova, M. - Llano, M. - Dostalkova, A. - Krizova, I. - Ruml, T. - Ulbrich, P.
PY  - 2018
TI  - Conserved cysteines in Mason-Pfizer monkey virus capsid protein are essential for infectious mature particle formation
JF  - Virology
VL  - 521
IS  - AUG
SP  - 108-117
EP  - 108-117
PB  - ACADEMIC PRESS INC ELSEVIER SCIENCE
SN  - 00426822
KW  - M-PMV capsid
KW  - Cysteine mutagenesis
KW  - Retrovirus assembly
KW  - Virus core stability
KW  - M-PMV infectivity
N2  - Retrovirus assembly is driven mostly by Gag polyprotein oligomerization, which is mediated by inter and intra protein protein interactions among its capsid (CA) domains. Mason-Pfizer monkey virus (M-PMV) CA contains three cysteines (C82, C193 and C213), where the latter two are highly conserved among most retroviruses. To determine the importance of these cysteines, we introduced mutations of these residues in both bacterial and proviral vectors and studied their impact on the M-PMV life cycle. These studies revealed that the presence of both conserved cysteines of M-PMV CA is necessary for both proper assembly and virus infectivity. Our findings suggest a crucial role of these cysteines in the formation of infectious mature particles.
ER  -

PICHALOVA, R.; Tibor FÜZIK; B. VOKATA; M. RUMLOVA; M. LLANO; A. DOSTALKOVA; I. KRIZOVA; T. RUML a P. ULBRICH. Conserved cysteines in Mason-Pfizer monkey virus capsid protein are essential for infectious mature particle formation. \textit{Virology}. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2018, roč.~521, AUG, s.~108-117. ISSN~0042-6822. Dostupné z: https://doi.org/10.1016/j.virol.2018.06.001.

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