Na+, K+/H+ antiporters regulate the pH of endoplasmic reticulum
and auxin-mediated development

J 2018

Na+, K+/H+ antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development

FAN, L.G.; L. ZHAO; W. HU; W.N. LI; O. NOVAK et al.

Základní údaje

Originální název

Na+, K+/H+ antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development

Autoři

FAN, L.G.; L. ZHAO; W. HU; W.N. LI; O. NOVAK; M. STRNAD; Sibu SIMON; J. FRIML; J.B. SHEN; L.W. JIANG a Q.S. QIU

Vydání

Plant, cell and environment, Oxford, Blackwell Science, 2018, 0140-7791

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.624

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/18:00101773

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Arabidopsis; endosomal Na+K+/H+ antiporters; AtNHX5; AtNHX6; PIN5; pH

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 3. 2019 11:28, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

AtNHX5 and AtNHX6 are endosomal Na+,K+/H+ antiporters that are critical for growth and development in Arabidopsis, but the mechanism behind their action remains unknown. Here, we report that AtNHX5 and AtNHX6, functioning as H+ leak, control auxin homeostasis and auxinmediated development. We found that nhx5 nhx6 exhibited growth variations of auxin-related defects. We further showed that nhx5 nhx6 was affected in auxin homeostasis. Genetic analysis showed that AtNHX5 and AtNHX6 were required for the function of the endoplasmic reticulum (ER)-localized auxin transporter PIN5. Although AtNHX5 and AtNHX6 were colocalized with PIN5 at ER, they did not interact directly. Instead, the conserved acidic residues in AtNHX5 and AtNHX6, which are essential for exchange activity, were required for PIN5 function. AtNHX5 and AtNHX6 regulated the pH in ER. Overall, AtNHX5 and AtNHX6 may regulate auxin transport across the ER via the pH gradient created by their transport activity. H+-leak pathway provides a fine-tuning mechanism that controls cellular auxin fluxes.

Návaznosti

GA13-40637S, projekt VaV

Název: Genetické studie k identifikaci molekulárních mechanizmů buněčné polarity a auxinového transportu v rostlinách

Investor: Grantová agentura ČR, Genetické studie k identifikaci molekulárních mechanizmu bunecné polarity a auxinového transportu v rostlinách

Citovat

FAN, L.G.; L. ZHAO; W. HU; W.N. LI; O. NOVAK; M. STRNAD; Sibu SIMON; J. FRIML; J.B. SHEN; L.W. JIANG a Q.S. QIU. Na+, K+/H+ antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development. Plant, cell and environment. Oxford: Blackwell Science, 2018, roč. 41, č. 4, s. 850-864. ISSN 0140-7791. Dostupné z: https://doi.org/10.1111/pce.13153.

@article{1505317,
   author = {Fan, L.G. and Zhao, L. and Hu, W. and Li, W.N. and Novak, O. and Strnad, M. and Simon, Sibu and Friml, J. and Shen, J.B. and Jiang, L.W. and Qiu, Q.S.},
   article_location = {Oxford},
   article_number = {4},
   doi = {https://doi.org/10.1111/pce.13153},
   keywords = {Arabidopsis; endosomal Na+K+/H+ antiporters; AtNHX5; AtNHX6; PIN5; pH},
   language = {eng},
   issn = {0140-7791},
   journal = {Plant, cell and environment},
   title = {Na+, K+/H+ antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development},
   volume = {41},
   year = {2018}
}

TY  - JOUR
ID  - 1505317
AU  - Fan, L.G. - Zhao, L. - Hu, W. - Li, W.N. - Novak, O. - Strnad, M. - Simon, Sibu - Friml, J. - Shen, J.B. - Jiang, L.W. - Qiu, Q.S.
PY  - 2018
TI  - Na+, K+/H+ antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development
JF  - Plant, cell and environment
VL  - 41
IS  - 4
SP  - 850-864
EP  - 850-864
PB  - Blackwell Science
SN  - 01407791
KW  - Arabidopsis
KW  - endosomal Na+K+/H+ antiporters
KW  - AtNHX5
KW  - AtNHX6
KW  - PIN5
KW  - pH
N2  - AtNHX5 and AtNHX6 are endosomal Na+,K+/H+ antiporters that are critical for growth and development in Arabidopsis, but the mechanism behind their action remains unknown. Here, we report that AtNHX5 and AtNHX6, functioning as H+ leak, control auxin homeostasis and auxinmediated development. We found that nhx5 nhx6 exhibited growth variations of auxin-related defects. We further showed that nhx5 nhx6 was affected in auxin homeostasis. Genetic analysis showed that AtNHX5 and AtNHX6 were required for the function of the endoplasmic reticulum (ER)-localized auxin transporter PIN5. Although AtNHX5 and AtNHX6 were colocalized with PIN5 at ER, they did not interact directly. Instead, the conserved acidic residues in AtNHX5 and AtNHX6, which are essential for exchange activity, were required for PIN5 function. AtNHX5 and AtNHX6 regulated the pH in ER. Overall, AtNHX5 and AtNHX6 may regulate auxin transport across the ER via the pH gradient created by their transport activity. H+-leak pathway provides a fine-tuning mechanism that controls cellular auxin fluxes.
ER  -

FAN, L.G.; L. ZHAO; W. HU; W.N. LI; O. NOVAK; M. STRNAD; Sibu SIMON; J. FRIML; J.B. SHEN; L.W. JIANG a Q.S. QIU. Na+, K+/H+ antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development. \textit{Plant, cell and environment}. Oxford: Blackwell Science, 2018, roč.~41, č.~4, s.~850-864. ISSN~0140-7791. Dostupné z: https://doi.org/10.1111/pce.13153.

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