Binding and inhibitory effect of the food colorants Sunset
Yellow and Ponceau 4R on amyloid fibrillation of lysozyme

J 2019

Binding and inhibitory effect of the food colorants Sunset Yellow and Ponceau 4R on amyloid fibrillation of lysozyme

MILLAN, S.; L. SATISH; Krishnendu BERA a H. SAHOO

Základní údaje

Originální název

Binding and inhibitory effect of the food colorants Sunset Yellow and Ponceau 4R on amyloid fibrillation of lysozyme

Autoři

MILLAN, S.; L. SATISH; Krishnendu BERA a H. SAHOO

Vydání

New Journal of Chemistry, Cambridge, ROYAL SOC. CHEMISTRY, 2019, 1144-0546

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.288

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/19:00109308

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

HUMAN SERUM-ALBUMIN; MOLECULAR-DYNAMICS; ALLURA RED; PROTEIN; FLUORESCENCE; DOCKING; DYES; FIBRILLOGENESIS; CONFORMATION; CARMOISINE

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 3. 2020 21:23, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Amyloid fibrillogenesis of proteins is known to be the root cause of a large number of diseases like Parkinson's, Alzheimer's, and Huntington's disease, spongiform encephalopathy, amyloid polyneuropathy, type-II diabetes, etc. In the present work, the impact of Sunset Yellow (SY) and Ponceau 4R (P4R) food colorants on amyloid fibrillation of lysozyme (Lyz) under acidic pH and high temperature was studied using Thioflavin T (ThT) fluorescence, Congo Red (CR) assay, circular dichroism (CD) spectroscopy, transmission electron microscopy (TEM) and field emission scanning electron microscopy (FESEM). The results illustrated that SY and P4R inhibited the formation of amyloid fibrils. In addition, the binding between food colorants (SY and P4R) and Lyz was investigated using various spectroscopic methods such as fluorescence, absorption and CD spectroscopy along with theoretical approaches (such as docking and simulation). The binding results suggested that P4R has a remarkably higher binding affinity for Lyz in comparison to SY. In summary, we hope the in vitro fibrillation and interaction studies in the presence of food colorants (SY and P4R) are helpful to gain a better understanding about the mechanism of amyloid fibril formation and interaction at the molecular level.

Citovat

MILLAN, S.; L. SATISH; Krishnendu BERA a H. SAHOO. Binding and inhibitory effect of the food colorants Sunset Yellow and Ponceau 4R on amyloid fibrillation of lysozyme. New Journal of Chemistry. Cambridge: ROYAL SOC. CHEMISTRY, 2019, roč. 43, č. 9, s. 3956-3968. ISSN 1144-0546. Dostupné z: https://doi.org/10.1039/c8nj05827j.

@article{1506736,
   author = {Millan, S. and Satish, L. and Bera, Krishnendu and Sahoo, H.},
   article_location = {Cambridge},
   article_number = {9},
   doi = {https://doi.org/10.1039/c8nj05827j},
   keywords = {HUMAN SERUM-ALBUMIN; MOLECULAR-DYNAMICS; ALLURA RED; PROTEIN; FLUORESCENCE; DOCKING; DYES; FIBRILLOGENESIS; CONFORMATION; CARMOISINE},
   language = {eng},
   issn = {1144-0546},
   journal = {New Journal of Chemistry},
   title = {Binding and inhibitory effect of the food colorants Sunset Yellow and Ponceau 4R on amyloid fibrillation of lysozyme},
   url = {https://pubs.rsc.org/en/content/articlelanding/2019/NJ/C8NJ05827J#!divAbstract},
   volume = {43},
   year = {2019}
}

TY  - JOUR
ID  - 1506736
AU  - Millan, S. - Satish, L. - Bera, Krishnendu - Sahoo, H.
PY  - 2019
TI  - Binding and inhibitory effect of the food colorants Sunset Yellow and Ponceau 4R on amyloid fibrillation of lysozyme
JF  - New Journal of Chemistry
VL  - 43
IS  - 9
SP  - 3956-3968
EP  - 3956-3968
PB  - ROYAL SOC. CHEMISTRY
SN  - 11440546
KW  - HUMAN SERUM-ALBUMIN
KW  - MOLECULAR-DYNAMICS
KW  - ALLURA RED
KW  - PROTEIN
KW  - FLUORESCENCE
KW  - DOCKING
KW  - DYES
KW  - FIBRILLOGENESIS
KW  - CONFORMATION
KW  - CARMOISINE
UR  - https://pubs.rsc.org/en/content/articlelanding/2019/NJ/C8NJ05827J#!divAbstract
L2  - https://pubs.rsc.org/en/content/articlelanding/2019/NJ/C8NJ05827J#!divAbstract
N2  - Amyloid fibrillogenesis of proteins is known to be the root cause of a large number of diseases like Parkinson's, Alzheimer's, and Huntington's disease, spongiform encephalopathy, amyloid polyneuropathy, type-II diabetes, etc. In the present work, the impact of Sunset Yellow (SY) and Ponceau 4R (P4R) food colorants on amyloid fibrillation of lysozyme (Lyz) under acidic pH and high temperature was studied using Thioflavin T (ThT) fluorescence, Congo Red (CR) assay, circular dichroism (CD) spectroscopy, transmission electron microscopy (TEM) and field emission scanning electron microscopy (FESEM). The results illustrated that SY and P4R inhibited the formation of amyloid fibrils. In addition, the binding between food colorants (SY and P4R) and Lyz was investigated using various spectroscopic methods such as fluorescence, absorption and CD spectroscopy along with theoretical approaches (such as docking and simulation). The binding results suggested that P4R has a remarkably higher binding affinity for Lyz in comparison to SY. In summary, we hope the in vitro fibrillation and interaction studies in the presence of food colorants (SY and P4R) are helpful to gain a better understanding about the mechanism of amyloid fibril formation and interaction at the molecular level.
ER  -

MILLAN, S.; L. SATISH; Krishnendu BERA a H. SAHOO. Binding and inhibitory effect of the food colorants Sunset Yellow and Ponceau 4R on amyloid fibrillation of lysozyme. \textit{New Journal of Chemistry}. Cambridge: ROYAL SOC. CHEMISTRY, 2019, roč.~43, č.~9, s.~3956-3968. ISSN~1144-0546. Dostupné z: https://doi.org/10.1039/c8nj05827j.

Přehled o publikaci