Gold nanoparticles as labels for immunochemical analysis using
laser ablation inductively coupled plasma mass spectrometry

J 2019

Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry

TVRDOŇOVÁ, Michaela; Marcela VLČNOVSKÁ; Lucie VANICKOVA; Viktor KANICKÝ; Vojtěch ADAM et. al.

Základní údaje

Originální název

Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry

Autoři

TVRDOŇOVÁ, Michaela; Marcela VLČNOVSKÁ; Lucie VANICKOVA; Viktor KANICKÝ; Vojtěch ADAM; Lena ASCHER; Norbert JAKUBOWSKI; Markéta VACULOVIČOVÁ a Tomáš VACULOVIČ

Vydání

Analytical and Bioanalytical chemistry, HEIDELBERG, SPRINGER HEIDELBERG, 2019, 1618-2642

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.637

Kód RIV

RIV/00216224:14310/19:00107331

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1007/s00216-018-1300-7

UT WoS

000456132700003

EID Scopus

2-s2.0-85051727664

Klíčová slova anglicky

Dot blot; Antibody labelling; Gold nanoparticles; LA-ICP-MS; Immunochemistry

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 4. 2020 10:33, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.

Návaznosti

GA17-12774S, projekt VaV

Název: Využití vícenásobného značení pomocí kovových nanočástic pro bio-zobrazování

Investor: Grantová agentura ČR, Využití vícenásobného značení pomocí kovových nanočástic pro bio-zobrazování

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

TVRDOŇOVÁ, Michaela; Marcela VLČNOVSKÁ; Lucie VANICKOVA; Viktor KANICKÝ; Vojtěch ADAM; Lena ASCHER; Norbert JAKUBOWSKI; Markéta VACULOVIČOVÁ a Tomáš VACULOVIČ. Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry. Analytical and Bioanalytical chemistry. HEIDELBERG: SPRINGER HEIDELBERG, 2019, roč. 411, č. 3, s. 559-564. ISSN 1618-2642. Dostupné z: https://doi.org/10.1007/s00216-018-1300-7.

@article{1512417,
   author = {Tvrdoňová, Michaela and Vlčnovská, Marcela and Vanickova, Lucie and Kanický, Viktor and Adam, Vojtěch and Ascher, Lena and Jakubowski, Norbert and Vaculovičová, Markéta and Vaculovič, Tomáš},
   article_location = {HEIDELBERG},
   article_number = {3},
   doi = {https://doi.org/10.1007/s00216-018-1300-7},
   keywords = {Dot blot; Antibody labelling; Gold nanoparticles; LA-ICP-MS; Immunochemistry},
   language = {eng},
   issn = {1618-2642},
   journal = {Analytical and Bioanalytical chemistry},
   title = {Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry},
   url = {http://dx.doi.org/10.1007/s00216-018-1300-7},
   volume = {411},
   year = {2019}
}

TY  - JOUR
ID  - 1512417
AU  - Tvrdoňová, Michaela - Vlčnovská, Marcela - Vanickova, Lucie - Kanický, Viktor - Adam, Vojtěch - Ascher, Lena - Jakubowski, Norbert - Vaculovičová, Markéta - Vaculovič, Tomáš
PY  - 2019
TI  - Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry
JF  - Analytical and Bioanalytical chemistry
VL  - 411
IS  - 3
SP  - 559-564
EP  - 559-564
PB  - SPRINGER HEIDELBERG
SN  - 16182642
KW  - Dot blot
KW  - Antibody labelling
KW  - Gold nanoparticles
KW  - LA-ICP-MS
KW  - Immunochemistry
UR  - http://dx.doi.org/10.1007/s00216-018-1300-7
L2  - http://dx.doi.org/10.1007/s00216-018-1300-7
N2  - In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.
ER  -

TVRDOŇOVÁ, Michaela; Marcela VLČNOVSKÁ; Lucie VANICKOVA; Viktor KANICKÝ; Vojtěch ADAM; Lena ASCHER; Norbert JAKUBOWSKI; Markéta VACULOVIČOVÁ a Tomáš VACULOVIČ. Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry. \textit{Analytical and Bioanalytical chemistry}. HEIDELBERG: SPRINGER HEIDELBERG, 2019, roč.~411, č.~3, s.~559-564. ISSN~1618-2642. Dostupné z: https://doi.org/10.1007/s00216-018-1300-7.

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