2019
Diclofenac as an environmental threat: Impact on the photosynthetic processes of Lemna minor chloroplasts
HÁJKOVÁ, Markéta, Marie KUMMEROVÁ, Štěpán ZEZULKA, Petr BABULA, Peter VÁCZI et. al.Základní údaje
Originální název
Diclofenac as an environmental threat: Impact on the photosynthetic processes of Lemna minor chloroplasts
Název česky
Diklofenak jako environmentální hrozba: Dopad na fotosyntetické procesy chloroplastů Lemna minor
Název anglicky
Diclofenac as an environmental threat: Impact on the photosynthetic processes of Lemna minor chloroplasts
Autoři
HÁJKOVÁ, Markéta (203 Česká republika, garant, domácí), Marie KUMMEROVÁ (203 Česká republika, domácí), Štěpán ZEZULKA (203 Česká republika, domácí), Petr BABULA (203 Česká republika, domácí) a Peter VÁCZI (703 Slovensko, domácí)
Vydání
Chemosphere, OXFORD, PERGAMON-ELSEVIER SCIENCE LTD, 2019, 0045-6535
Další údaje
Jazyk
čeština
Typ výsledku
Článek v odborném periodiku
Obor
10611 Plant sciences, botany
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 5.778
Kód RIV
RIV/00216224:14310/19:00107335
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000466249600100
Klíčová slova česky
Diklofenak; chloroplasty okřehku; fotosystémy II a I; RuBisCO; oxidativní stres;
Klíčová slova anglicky
Diclofenac; Duckweed chloroplasts; Photosystems II and I; RuBisCO; Oxidative stress;
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 17. 4. 2020 16:27, Mgr. Marie Šípková, DiS.
V originále
Mechanisms of pharmaceuticals action on biochemical and physiological processes in plants that determine plant growth and development are still mostly unknown. This study deals with the effects of non-steroidal anti-inflammatory drug diclofenac (DCF) on photosynthesis as an essential anabolic process. Changes in primary and secondary photosynthetic processes were assessed in chloroplasts isolated from Lemna minor exposed to 1, 10, 100, and 1000 uM DCF. Decreases in the potential and effective quantum yields of photosystem II (FV/FM by 21%, PhiII by 44% compared to control), changes in non-photochemical fluorescence quenching (NPQ), and a substantial drop in Hill reaction activity (by 73%), especially under 1000 uM DCF, were found. Limitation of electron transport through photosystem II was confirmed by increased fluorescence signals in steps J and I (by 50% and 23%, respectively, under 1000 uM DCF) in OJIP fluorescence transient. Photosystem I exhibited changes only in the redox state of P700 reaction centres (decrease in Pm by 10%, increase in reduced P700 by 5% under 1000 uM DCF). Similarly, RuBisCO activity was only lowered by 30% under 1000 uM DCF. In contrast, a significant increase in reactive oxygen and nitrogen species (by 116% and 157%, respectively) was observed under 10 uM DCF, and lipid peroxidation increased even at 1 uM DCF (by nearly seven times compared to the control). Results demonstrate the ability of environmentally relevant DCF concentrations to induce oxidative stress in isolated duckweed chloroplasts; however, photosynthetic processes were affected considerably only by the highest DCF treatments.
Anglicky
Mechanisms of pharmaceuticals action on biochemical and physiological processes in plants that determine plant growth and development are still mostly unknown. This study deals with the effects of non-steroidal anti-inflammatory drug diclofenac (DCF) on photosynthesis as an essential anabolic process. Changes in primary and secondary photosynthetic processes were assessed in chloroplasts isolated from Lemna minor exposed to 1, 10, 100, and 1000 uM DCF. Decreases in the potential and effective quantum yields of photosystem II (FV/FM by 21%, PhiII by 44% compared to control), changes in non-photochemical fluorescence quenching (NPQ), and a substantial drop in Hill reaction activity (by 73%), especially under 1000 uM DCF, were found. Limitation of electron transport through photosystem II was confirmed by increased fluorescence signals in steps J and I (by 50% and 23%, respectively, under 1000 uM DCF) in OJIP fluorescence transient. Photosystem I exhibited changes only in the redox state of P700 reaction centres (decrease in Pm by 10%, increase in reduced P700 by 5% under 1000 uM DCF). Similarly, RuBisCO activity was only lowered by 30% under 1000 uM DCF. In contrast, a significant increase in reactive oxygen and nitrogen species (by 116% and 157%, respectively) was observed under 10 uM DCF, and lipid peroxidation increased even at 1 uM DCF (by nearly seven times compared to the control). Results demonstrate the ability of environmentally relevant DCF concentrations to induce oxidative stress in isolated duckweed chloroplasts; however, photosynthetic processes were affected considerably only by the highest DCF treatments.
Návaznosti
| GF17-33746L, projekt VaV |
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