Fluorescent Capillary Electrophoresis Is Superior to Culture in
Detecting Candida Species from Samples of Urinary Catheters and
Ureteral Stents with Mono- or Polyfungal Biofilm Growth

J 2019

Fluorescent Capillary Electrophoresis Is Superior to Culture in Detecting Candida Species from Samples of Urinary Catheters and Ureteral Stents with Mono- or Polyfungal Biofilm Growth

OBRUCOVA, H., Iva KOTÁSKOVÁ, R. TIHELKOVA, Veronika HOLÁ, Filip RŮŽIČKA et. al.

Základní údaje

Originální název

Fluorescent Capillary Electrophoresis Is Superior to Culture in Detecting Candida Species from Samples of Urinary Catheters and Ureteral Stents with Mono- or Polyfungal Biofilm Growth

Autoři

OBRUCOVA, H. (203 Česká republika), Iva KOTÁSKOVÁ (203 Česká republika, domácí), R. TIHELKOVA (203 Česká republika), Veronika HOLÁ (203 Česká republika, domácí), Filip RŮŽIČKA (203 Česká republika, domácí) a Tomáš FREIBERGER (203 Česká republika, garant, domácí)

Vydání

Journal of Clinical Microbiology, Washington, American Society for Microbiology, 2019, 0095-1137

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.897

Kód RIV

RIV/00216224:14740/19:00108471

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1128/JCM.01861-18

UT WoS

000462714100028

Klíčová slova anglicky

Candida; biofilms; capillary electrophoresis; fungi; panfungal PCR; polyfungal sample; ureteral stents; urinary catheters

Štítky

14110113, 14110114, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 5. 2020 13:28, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Molecular techniques in fungal detection and identification represent an efficient complementary diagnostic tool which is increasingly used to overcome limitations of routinely used culture techniques. The aim of this study was to characterize Candida sp. representation in samples from urine, urinary catheter, and ureteral stent biofilm using ITS2 ribosomal DNA (rDNA) amplification followed by fluorescent capillary electrophoresis (f-ITS2-PCR-CE) and to compare the results with those obtained by culture. A total of 419 samples were analyzed, and 106 (25.2%) were found positive, out of which 17 (16%) were polyfungal. The positivity rate did not differ between samples from catheters and stents (23.6% versus 20.9%) or between catheter and stent corresponding urine samples (40.2% versus 30.2%). Ten different Candida species were detected, with Candida parapsilosis (31.4%), Candida albicans (26.5%), and Candida tropicalis (12.4%) predominating. f-ITS2-PCR-CE was evaluated as substantially less time-consuming and 8.3 times more sensitive than the routinely applied culture technique with 1 mu l of urine/sonicated fluid inoculated, detecting 67 (19.9%) versus 8 (2.4%) positive samples out of 337 initially analyzed samples. The culture sensitivity considerably improved to 1.7 times lower than that of f-ITS2-PCR-CE after the inoculation volume was increased to 100 mu l in the additional 82 samples. Moreover, the molecular technique, unlike routine cultivation, enabled precise pathogen composition determination in polymicrobial samples. In conclusion, the f-ITS2-PCR-CE method was shown to be a quick and efficient tool for culture-independent detection and identification of fungi in urinary tract-related samples, demonstrating a higher sensitivity than culture.

Návaznosti

MUNI/A/0925/2017, interní kód MU

Název: Poruchy tvorby protilátek a komplementového systému

Investor: Masarykova univerzita, Poruchy tvorby protilátek a komplementového systému, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

MUNI/A/1189/2018, interní kód MU

Název: Původci nozokomiálních nákaz a možnosti jejich diagnostiky a terapie (Akronym: PNNMDT)

Investor: Masarykova univerzita, Původci nozokomiálních nákaz a možnosti jejich diagnostiky a terapie, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

NV16-31593A, projekt VaV

Název: Polymikrobiální biofilm jako komplexní mikrobiom v rámci léčby nozokomiálních infekcí

Citovat

OBRUCOVA, H., Iva KOTÁSKOVÁ, R. TIHELKOVA, Veronika HOLÁ, Filip RŮŽIČKA a Tomáš FREIBERGER. Fluorescent Capillary Electrophoresis Is Superior to Culture in Detecting Candida Species from Samples of Urinary Catheters and Ureteral Stents with Mono- or Polyfungal Biofilm Growth. Journal of Clinical Microbiology. Washington: American Society for Microbiology, 2019, roč. 57, č. 4, s. nestrankovano, 10 s. ISSN 0095-1137. Dostupné z: https://dx.doi.org/10.1128/JCM.01861-18.

@article{1531658,
   author = {Obrucova, H. and Kotásková, Iva and Tihelkova, R. and Holá, Veronika and Růžička, Filip and Freiberger, Tomáš},
   article_location = {Washington},
   article_number = {4},
   doi = {http://dx.doi.org/10.1128/JCM.01861-18},
   keywords = {Candida; biofilms; capillary electrophoresis; fungi; panfungal PCR; polyfungal sample; ureteral stents; urinary catheters},
   language = {eng},
   issn = {0095-1137},
   journal = {Journal of Clinical Microbiology},
   title = {Fluorescent Capillary Electrophoresis Is Superior to Culture in Detecting Candida Species from Samples of Urinary Catheters and Ureteral Stents with Mono- or Polyfungal Biofilm Growth},
   url = {https://jcm.asm.org/content/57/4/e01861-18},
   volume = {57},
   year = {2019}
}

TY  - JOUR
ID  - 1531658
AU  - Obrucova, H. - Kotásková, Iva - Tihelkova, R. - Holá, Veronika - Růžička, Filip - Freiberger, Tomáš
PY  - 2019
TI  - Fluorescent Capillary Electrophoresis Is Superior to Culture in Detecting Candida Species from Samples of Urinary Catheters and Ureteral Stents with Mono- or Polyfungal Biofilm Growth
JF  - Journal of Clinical Microbiology
VL  - 57
IS  - 4
SP  - nestrankovano
EP  - nestrankovano
PB  - American Society for Microbiology
SN  - 00951137
KW  - Candida
KW  - biofilms
KW  - capillary electrophoresis
KW  - fungi
KW  - panfungal PCR
KW  - polyfungal sample
KW  - ureteral stents
KW  - urinary catheters
UR  - https://jcm.asm.org/content/57/4/e01861-18
L2  - https://jcm.asm.org/content/57/4/e01861-18
N2  - Molecular techniques in fungal detection and identification represent an efficient complementary diagnostic tool which is increasingly used to overcome limitations of routinely used culture techniques. The aim of this study was to characterize Candida sp. representation in samples from urine, urinary catheter, and ureteral stent biofilm using ITS2 ribosomal DNA (rDNA) amplification followed by fluorescent capillary electrophoresis (f-ITS2-PCR-CE) and to compare the results with those obtained by culture. A total of 419 samples were analyzed, and 106 (25.2%) were found positive, out of which 17 (16%) were polyfungal. The positivity rate did not differ between samples from catheters and stents (23.6% versus 20.9%) or between catheter and stent corresponding urine samples (40.2% versus 30.2%). Ten different Candida species were detected, with Candida parapsilosis (31.4%), Candida albicans (26.5%), and Candida tropicalis (12.4%) predominating. f-ITS2-PCR-CE was evaluated as substantially less time-consuming and 8.3 times more sensitive than the routinely applied culture technique with 1 mu l of urine/sonicated fluid inoculated, detecting 67 (19.9%) versus 8 (2.4%) positive samples out of 337 initially analyzed samples. The culture sensitivity considerably improved to 1.7 times lower than that of f-ITS2-PCR-CE after the inoculation volume was increased to 100 mu l in the additional 82 samples. Moreover, the molecular technique, unlike routine cultivation, enabled precise pathogen composition determination in polymicrobial samples. In conclusion, the f-ITS2-PCR-CE method was shown to be a quick and efficient tool for culture-independent detection and identification of fungi in urinary tract-related samples, demonstrating a higher sensitivity than culture.
ER  -

OBRUCOVA, H., Iva KOTÁSKOVÁ, R. TIHELKOVA, Veronika HOLÁ, Filip RŮŽIČKA a Tomáš FREIBERGER. Fluorescent Capillary Electrophoresis Is Superior to Culture in Detecting Candida Species from Samples of Urinary Catheters and Ureteral Stents with Mono- or Polyfungal Biofilm Growth. \textit{Journal of Clinical Microbiology}. Washington: American Society for Microbiology, 2019, roč.~57, č.~4, s.~nestrankovano, 10 s. ISSN~0095-1137. Dostupné z: https://dx.doi.org/10.1128/JCM.01861-18.

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