Butyrate interacts with benzo [a] pyrene to alter expression
and activities of xenobiotic metabolizing enzymes involved in
metabolism of carcinogens within colon epithelial cell models

J 2019

Butyrate interacts with benzo [a] pyrene to alter expression and activities of xenobiotic metabolizing enzymes involved in metabolism of carcinogens within colon epithelial cell models

ZAPLETAL, Ondřej, Jiřina PROCHÁZKOVÁ, Vít DUBEC, Jiřina HOFMANOVÁ, Alois KOZUBÍK et. al.

Základní údaje

Originální název

Butyrate interacts with benzo [a] pyrene to alter expression and activities of xenobiotic metabolizing enzymes involved in metabolism of carcinogens within colon epithelial cell models

Autoři

ZAPLETAL, Ondřej (203 Česká republika, domácí), Jiřina PROCHÁZKOVÁ (203 Česká republika), Vít DUBEC (203 Česká republika, domácí), Jiřina HOFMANOVÁ (203 Česká republika), Alois KOZUBÍK (203 Česká republika) a Jan VONDRÁČEK (203 Česká republika)

Vydání

Toxicology, Clare, ELSEVIER IRELAND LTD, 2019, 0300-483X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30108 Toxicology

Stát vydavatele

Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 4.099

Kód RIV

RIV/00216224:14310/19:00109768

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.tox.2018.11.001

UT WoS

000457811600001

EID Scopus

2-s2.0-85056908262

Klíčová slova anglicky

Butyrate; Polycyclic aromatic hydrocarbons; NAD(P)H:quinone oxidoreductase 1; N-acetyltransferases; UDP-glucuronosyltransferases; Colon epithelium

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 1. 2020 12:44, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Butyrate helps to maintain colon homeostasis and exhibits chemopreventive effects in colon epithelium. We examined the interactive effects of butyrate and benzo[a]pyrene (BaP), dietary carcinogen, in regulation of expression of a panel of phase I and II xenobiotic metabolizing enzymes (XMEs) in human colon cells. In human colon carcinoma HCT-116 and HT-29 cell lines, butyrate alone increased mRNA levels of some enzymes, such as N-acetyltransferases (in particular NAT2). In combination with BaP, butyrate potentiated induction of cytochrome P450 family 1 enzymes (CYP1A1), aldo-keto reductases (AKR1C1) or UDP-glucuronosyltransferases (UGT1A1). There were some notable differences between cell lines, as butyrate potentiated induction of NAD(P) H:quinone oxidoreductase 1 (NQO1) and UGT1A4 only in HCT-116 cells, and it even repressed AKR1C3 induction in HT-29 cells. Butyrate also promoted induction of CYP1, NQO1, NAT2, UGT1A1 or UGT1A4 in human colon Caco-2 cells, in a differentiation-dependent manner. Differentiated Caco-2 Cells exhibited a higher inducibility of selected XME genes than undifferentiated cells. Butyrate increased induction of enzymatic activities of NATs, NQO1 and UGTs by BaP in HCT-116 and HT29 cells, whereas in differentiated Caco-2 cells it helped to increase only enzymatic activity of NQO1 and UGTs. Together, the present data suggest that butyrate may modulate expression/activities of several enzymes involved in metabolism of carcinogens in colon. In some cases (NAT2, UGT1 A1), this was linked to inhibition of histone deacetylases (HDAC), as confirmed by using HDAC inhibitor trichostatin A. These results may have implications for our understanding of the role of butyrate in regulation of XMEs and carcinogen metabolism in colon.

Citovat

ZAPLETAL, Ondřej, Jiřina PROCHÁZKOVÁ, Vít DUBEC, Jiřina HOFMANOVÁ, Alois KOZUBÍK a Jan VONDRÁČEK. Butyrate interacts with benzo [a] pyrene to alter expression and activities of xenobiotic metabolizing enzymes involved in metabolism of carcinogens within colon epithelial cell models. Toxicology. Clare: ELSEVIER IRELAND LTD, 2019, roč. 412, JAN, s. 1-11. ISSN 0300-483X. Dostupné z: https://dx.doi.org/10.1016/j.tox.2018.11.001.

@article{1534737,
   author = {Zapletal, Ondřej and Procházková, Jiřina and Dubec, Vít and Hofmanová, Jiřina and Kozubík, Alois and Vondráček, Jan},
   article_location = {Clare},
   article_number = {JAN},
   doi = {http://dx.doi.org/10.1016/j.tox.2018.11.001},
   keywords = {Butyrate; Polycyclic aromatic hydrocarbons; NAD(P)H:quinone oxidoreductase 1; N-acetyltransferases; UDP-glucuronosyltransferases; Colon epithelium},
   language = {eng},
   issn = {0300-483X},
   journal = {Toxicology},
   title = {Butyrate interacts with benzo [a] pyrene to alter expression and activities of xenobiotic metabolizing enzymes involved in metabolism of carcinogens within colon epithelial cell models},
   url = {https://www.sciencedirect.com/science/article/pii/S0300483X18305729},
   volume = {412},
   year = {2019}
}

TY  - JOUR
ID  - 1534737
AU  - Zapletal, Ondřej - Procházková, Jiřina - Dubec, Vít - Hofmanová, Jiřina - Kozubík, Alois - Vondráček, Jan
PY  - 2019
TI  - Butyrate interacts with benzo [a] pyrene to alter expression and activities of xenobiotic metabolizing enzymes involved in metabolism of carcinogens within colon epithelial cell models
JF  - Toxicology
VL  - 412
IS  - JAN
SP  - 1-11
EP  - 1-11
PB  - ELSEVIER IRELAND LTD
SN  - 0300483X
KW  - Butyrate
KW  - Polycyclic aromatic hydrocarbons
KW  - NAD(P)H:quinone oxidoreductase 1
KW  - N-acetyltransferases
KW  - UDP-glucuronosyltransferases
KW  - Colon epithelium
UR  - https://www.sciencedirect.com/science/article/pii/S0300483X18305729
L2  - https://www.sciencedirect.com/science/article/pii/S0300483X18305729
N2  - Butyrate helps to maintain colon homeostasis and exhibits chemopreventive effects in colon epithelium. We examined the interactive effects of butyrate and benzo[a]pyrene (BaP), dietary carcinogen, in regulation of expression of a panel of phase I and II xenobiotic metabolizing enzymes (XMEs) in human colon cells. In human colon carcinoma HCT-116 and HT-29 cell lines, butyrate alone increased mRNA levels of some enzymes, such as N-acetyltransferases (in particular NAT2). In combination with BaP, butyrate potentiated induction of cytochrome P450 family 1 enzymes (CYP1A1), aldo-keto reductases (AKR1C1) or UDP-glucuronosyltransferases (UGT1A1). There were some notable differences between cell lines, as butyrate potentiated induction of NAD(P) H:quinone oxidoreductase 1 (NQO1) and UGT1A4 only in HCT-116 cells, and it even repressed AKR1C3 induction in HT-29 cells. Butyrate also promoted induction of CYP1, NQO1, NAT2, UGT1A1 or UGT1A4 in human colon Caco-2 cells, in a differentiation-dependent manner. Differentiated Caco-2 Cells exhibited a higher inducibility of selected XME genes than undifferentiated cells. Butyrate increased induction of enzymatic activities of NATs, NQO1 and UGTs by BaP in HCT-116 and HT29 cells, whereas in differentiated Caco-2 cells it helped to increase only enzymatic activity of NQO1 and UGTs. Together, the present data suggest that butyrate may modulate expression/activities of several enzymes involved in metabolism of carcinogens in colon. In some cases (NAT2, UGT1 A1), this was linked to inhibition of histone deacetylases (HDAC), as confirmed by using HDAC inhibitor trichostatin A. These results may have implications for our understanding of the role of butyrate in regulation of XMEs and carcinogen metabolism in colon.
ER  -

ZAPLETAL, Ondřej, Jiřina PROCHÁZKOVÁ, Vít DUBEC, Jiřina HOFMANOVÁ, Alois KOZUBÍK a Jan VONDRÁČEK. Butyrate interacts with benzo [a] pyrene to alter expression and activities of xenobiotic metabolizing enzymes involved in metabolism of carcinogens within colon epithelial cell models. \textit{Toxicology}. Clare: ELSEVIER IRELAND LTD, 2019, roč.~412, JAN, s.~1-11. ISSN~0300-483X. Dostupné z: https://dx.doi.org/10.1016/j.tox.2018.11.001.

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